Mouse skin frozen sections were fixed with acetone, washed in 1X PBS, blocked with 5% goat serum/1% BSA for 30 minutes, and incubated with anti-MMP-9 polyclonal antibody (ABBIOTEC, San Diego, CA) as a primary antibody (diluted 1:200). FITC-conjugated goat antibody to rabbit IgG (Molecular Probes, Grand Island, NY) was used as a secondary antibody (diluted 1:1000) and was incubated for 40 minutes. For FcεRI detection, we used anti-mouse FcεRI PE (eBioscience, San Diego, CA). We mounted sections in ProLong Anti-Fade reagent (Molecular Probes) with 4’, 6-diamidino-2-phenylindole (DAPI). After overnight incubation at 4°C in the dark, images were obtained using a Zeiss LSM510 laser scanning confocal microscope coupled with an Axiovert 100 inverted stage microscope.
Anti mouse fcεri pe
Manufactured by Thermo Fisher Scientific
Anti-mouse FcεRI PE is a fluorescently labeled antibody that binds to the high-affinity receptor for the Fc region of IgE (FcεRI) on mouse cells. It can be used to detect and quantify FcεRI-expressing cells, such as mast cells and basophils, in flow cytometry applications.
Automatically generated - may contain errors
Lab products found in correlation
Prolong antifade reagent, by Thermo Fisher Scientific (2 mentions)
Lsm 510 confocal laser scanning microscope, by Zeiss (2 mentions)
Anti mmp 9 polyclonal antibody, by Abbiotec (2 mentions)
Fitc conjugated goat antibody to rabbit igg, by Thermo Fisher Scientific (2 mentions)
Axiovert 100 inverted stage microscope, by Zeiss (2 mentions)
2 protocols using anti mouse fcεri pe
1
Immunofluorescent Staining of Mouse Skin
2
Immunofluorescent Staining of Mouse Skin
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