Tergitol np 10

Manufactured by Merck Group

Sourced in India

Tergitol NP-10 is a non-ionic surfactant used in various laboratory applications. It functions as a wetting agent, emulsifier, and detergent. The product is soluble in water and can be used to facilitate the dispersion of hydrophobic substances in aqueous media.

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Tergitol (12 citations)

3 protocols using tergitol np 10

Purification of Membrane Proteins

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Yeast extract, tryptone, and glycerol were purchased from Fisher Scientific. Sodium cholate of >99% purity was obtained from Anatrace. Isopropyl-β-D-thiogalactopyranoside (IPTG), carbenicillin (disodium salt) and δ-aminolevulinic acid were from Research Products International Corp. DE52 media (preswollen diethylaminoethyl cellulose) was from Whatman. Bio-Beads SM-2 and Hydroxyapatite resins were from Bio-Rad. Dithiothreitol, Tergitol NP-10, nonaethylene glycol monododecyl ether (Brij), Cymal-5, Reactive Red 120 type 300-CL, Octyl Sepharose CL-4B, 2,6-ditertbutyl-4-methylphenol butylated hydroxytoluene (BHT), Trizma base, and lysozyme were from Sigma. DNase and the protease inhibitor cocktail were purchased from Roche Diagnostics Corp. (Indianapolis). 50% poly-ethylene glycol (PEG) 3350 solution, 10% w/v of Anapoe-C₁₀E₉ solution_, 1 M magnesium acetate solution, 1 M sodium cacodylate solution, and 1 M HCl solution were purchased from Hampton Research (California).

Yang Y., Bu W., Im S., Meagher J., Stuckey J, & Waskell L. (2018). Structure of cytochrome P450 2B4 with an acetate ligand and an active site hydrogen bond network similar to oxyferrous P450cam. Journal of inorganic biochemistry, 185, 17-25.

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Formulation of Drug-Loaded Nano-Carriers

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Materials were obtained from Sigma unless otherwise noted. The following were used: Pluronic F127 (Sigma # P2443), Pluronic F108 (Sigma # 542342), Pluronic F68 (Sigma # 412325), Polysorbate 20 (Amresco # M147-1L), Polysorbate 40 (Sigma # 1547936), Polysorbate 80 (VWR # EM-9490), Cremophor EL (Sigma # C5135), Cremophor RH40 (Sigma # 07076), Tergitol NP 9 (Sigma # NP9), Tergitol NP 10 (Sigma # NP10), Tergitol NP 40 (Sigma # NP40S), Brij 97 (Spectrum # B1679), Brij 35 (Spectrum # BR105), Brij L23 (Sigma # 16005), Brij O20 (Sigma # 436240), Vitamin K1 (VK1, VWR # AAAL10575-03), cyclosporine A (CsA, VWR # 89156-334), Testosterone undecanote (T-undec, Matrix # 099258), cholecalciferol (VWR, TCC0314), Retinol Palmitate (VWR # IC15652125), coenzyme Q10 (Sigma # 45-C9538), Docetaxel (LC Labs # D-1000), Paclitaxel (LC Labs # P-9600), Cabazitaxel (CTX, Proactive Molecular Research), Squalene (VWR # AAB20944-22), 5,9,14,18,23,27,32,36-Octabutoxy-2,3-naphthalocyanine (ONc, Sigma # 412074) 2,9,16,23-Tetra-tert-butyl-29H,31H-phthalocyanine (BPc, Sigma # 423157), Zinc 2,11,20,29-tetra-tert-butyl-2,3-naphthalocyanine (ZnBNc, Sigma # 432210), Castor oil (Sigma # 259853), methylene chloride (Fisher), Ethanol (Decon), ethylene glycol (VWR # 1071401), and sodium chloride (Macron # 7532-06).

Zhang Y., Song W., Geng J., Chitgupi U., Unsal H., Federizon J., Rzayev J., Sukumaran D.K., Alexandridis P, & Lovell J.F. (2016). Therapeutic surfactant-stripped frozen micelles. Nature Communications, 7, 11649.

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Biochemical Analysis of Paraoxonase-1

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5-bromo-4-chloro-3`-indolyphosphate (BCIP), alkaline phosphatase-labelled anti-mouse secondary antibody, lysozyme, nitro-blue-tetrazolium (NBT) reagent, paraoxon-ethyl, protease inhibitor cocktail, urea, m-cresol purple, δ-valerolactone and tergitol NP-10 were purchased from Sigma–Aldrich, Bangalore, India. Mouse anti-PON1 primary antibody and 5-thiobutyl butyrolactone (TBBL) was a kind gift from Dr. Richard W James, University of Geneva, Switzerland and Dr. Tawfik D.S, Department of Biological Chemistry, Weizmann Institute of Science, respectively. Stratagene's Quick change site directed mutagenesis kit was purchased from Agilent Technologies, Gurgaon, India. Protein molecular weight markers and Bradford reagent were purchased from Bio-Rad, Gurgaon, India. DNA primers (oligonucleotides) used were synthesized and purchased from Sigma Aldrich, Bangalore, India. Chlorpyrifos oxon (CPO) and diisopropylfluorophosphate (DFP) were purchased from Chem. Service, Inc., West Chester, PA, USA. All other reagents used were of analytical grade. Buffers used were prepared in double distilled water.

Aggarwal G., Prajapati R., Tripathy R.K., Bajaj P., Iyengar A.R., Sangamwar A.T, & Pande A.H. (2016). Toward Understanding the Catalytic Mechanism of Human Paraoxonase 1: Site-Specific Mutagenesis at Position 192. PLoS ONE, 11(2), e0147999.

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