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Antibodies to gapdh

Manufactured by R&D Systems

Antibodies to GAPDH are laboratory reagents used to detect and measure the presence of the glyceraldehyde 3-phosphate dehydrogenase (GAPDH) protein in biological samples. GAPDH is a widely expressed housekeeping gene that is involved in the glycolysis pathway. These antibodies can be used in various immunoassay techniques, such as Western blotting, immunohistochemistry, and ELISA, to study the expression and localization of GAPDH in cells and tissues.

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2 protocols using antibodies to gapdh

1

Immunoblotting and Immunofluorescence Reagents

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Antibody to LPL (SC-16657; Goat) was purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Antibodies to GAPDH and TNF-α receptor 1 (TNFR1) were purchased from R & D Systems (Minneapolis, MN). Protein estimation reagent, molecular weight standards, and PAGE reagents were bought from Bio-Rad (Hercules, CA). Cy2- and Cy3-conjugated secondary antibodies were purchased from Jackson Immunoresearch (West Grove, PA). HRP-conjugated secondary antibodies for immunoblotting and the phosphoserine (p-Serine) antibody were purchased from Abcam (Cambridge, MA). Mounting solutions for mounting of coverslips were purchased from Thomas Scientific (Swedesboro, NJ) or Vector Labs (Burlingame, CA). Rhodamine-phalloidin and all other chemicals were purchased from Sigma (St. Louis, MO).
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2

Antibody Acquisition and Characterization

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Antibody to L-plastin (SC-16657; Goat) was bought from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA). Antibodies to GAPDH was purchased from R & D Systems (Minneapolis, MN) and Sigma (St. Louis, MO). Protein estimation reagent, molecular weight standards for proteins, and PAGE reagents were bought from Bio-Rad. Cy2- and Cy3-conjugated secondary antibodies were purchased from Jackson Immunoresearch (West Grove, PA). HRP-conjugated secondary antibodies for immunoblotting were obtained from GE Healthcare. Antibody to phosphoserine (p-Serine) was bought from Zymed laboratories (61–8100) or Millipore (AB1603). Alizarin red solution was bought from Life-line Cell Technology (CM-0058; Fredrick, MD) Rhodamine-phalloidin and other chemicals were purchased from Sigma (St. Louis, MO).
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