HEK293 cells were seeded in clear-bottomed white 96-well plates and transfected in triplicate. For the SV40 assay, cells were transfected with 12 ng of pGL3-promoter firefly luciferase reporter construct (Promega), 5 ng of pRL-TK Renilla luciferase normalization control (Promega), and 16 ng of YFP-FOXP2 (wild-type or K674R or R553H mutant) or YFP control construct. For the SRPX2 assay, cells were transfected with 4.3 ng of SRPX2 luciferase reporter construct, 5 ng of pGL4.74 Renilla luciferase normalization control (Promega), and 45 ng of YFP-FOXP2 (wild-type or K674R or R553H mutant) or YFP control construct. After 48 h, luciferase activity was measured in a TECAN F200PRO microplate reader using the Dual-Luciferase Reporter Assay system (Promega).
Pgl3 promoter firefly luciferase reporter construct
Manufactured by Promega
The PGL3-promoter firefly luciferase reporter construct is a lab equipment product that consists of a plasmid vector containing the firefly luciferase gene under the control of a promoter sequence. This construct is designed to be used for the study of gene expression, with the luciferase gene serving as a reporter to quantify the activity of the promoter.
Automatically generated - may contain errors
2 protocols using pgl3 promoter firefly luciferase reporter construct
1
FOXP2 Regulates SV40 and SRPX2 Expression
2
Evaluating FOXP2 Regulation of SV40 and SRPX2
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HEK293 cells were seeded in clear-bottomed white 96-well plates and transfected in triplicate. For the SV40 assay, cells were transfected with 12 ng of pGL3-promoter firefly luciferase reporter construct containing the SV40 promoter (Promega), 5 ng of pRL-TK Renilla luciferase normalization control (Promega), and 16 ng of YFP-FOXP2 (wild-type or variant) or YFP control construct. For the SRPX2 assay, cells were transfected with 4.3 ng of SRPX2 luciferase reporter construct, 5 ng of pGL4.74 Renilla luciferase normalization control (Promega), and 45 ng of YFP-FOXP2 (wild-type or variant) or YFP control construct. After 48 h, luciferase activity was measured in a TECAN F200PRO microplate reader using the Dual-Luciferase Reporter Assay system (Promega).
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