The experimental steps of Western blot were conducted by our previous literature.21 (link) The primary antibodies listed below were incubated with the membranes at 4 °C and stayed overnight (at a 1:1,000 dilution): α-SMA, transforming growth factor (TGF)-β1, collagen-I, collagen-III, MMP-2, MMP-9, NLRP3, Caspase-1, GSDMD-N, interleukin (IL)-1β, IL-17A, and IL-18 (ABclonal Technology, Wuhan, China); ODC, SSAT, p-Smad-2, t-Smad-2, p-Smad-3, t-Smad-3, and Smad-7 (Cell Signaling Technology, Danvers, Massachusetts); and ubiquitin, β-actin, and β-tubulin (Santa Cruz Biotechnology). The membranes were incubated for 1 h at room temperature with secondary antibodies (diluted at 1:10,000, Proteintech, Wuhan, China). The specific complexes treated with an ECL kit (MultiSciences, Hangzhou, China) were detected using a multiplex fluorescent imaging system.
Gsdmd n
Manufactured by ABclonal
Sourced in China
GSDMD-N is a recombinant protein product produced by ABclonal. It represents the N-terminal domain of the GSDMD (Gasdermin D) protein. GSDMD is a key executioner of pyroptosis, a form of programmed cell death. The GSDMD-N product can be used for research purposes to study the structure and function of the GSDMD protein.
Automatically generated - may contain errors
Lab products found in correlation
Ubiquitin, by Santa Cruz Biotechnology (1 mentions)
Caspase 1, by ABclonal (1 mentions)
T smad3, by Cell Signaling Technology (1 mentions)
Smad7, by Cell Signaling Technology (1 mentions)
Il 17a, by ABclonal (1 mentions)
T smad2, by Cell Signaling Technology (1 mentions)
Ecl kit, by MultiSciences Biotech (1 mentions)
Collagen 1, by ABclonal (1 mentions)
P smad2, by Cell Signaling Technology (1 mentions)
P smad3, by Cell Signaling Technology (1 mentions)
α sma, by ABclonal (1 mentions)
β tubulin, by Santa Cruz Biotechnology (1 mentions)
β actin, by Santa Cruz Biotechnology (1 mentions)
Il 18, by ABclonal (1 mentions)
Nlrp3, by ABclonal (1 mentions)
Nf κb p65, by Wanlei (1 mentions)
Lipofiter 3, by Hanbio Biotechnology (1 mentions)
Il 18, by Wanlei (1 mentions)
Nlrp3, by Bioss Antibodies (1 mentions)
Cleaved caspase 1, by Wanlei (1 mentions)
2 protocols using gsdmd n
1
Western Blot Analysis of Key Signaling Pathways
2
Molecular Mechanisms of TRIM32-Mediated Inflammasome Activation
Check if the same lab product or an alternative is used in the 5 most similar protocols
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BAY 11-7082, bicinchoninic acid (BCA) kit, and penicillin/streptomycin were purchased from Beyotime (Shanghai, China). TRIzol reagent and DAPI were obtained from Sigma-Aldrich (USA). The cDNA First-Strand Synthesis Kit and SYBR Green Master Mix were purchased from Roche (Switzerland). LipoFiter 3.0 was obtained from Hanbio Biotechnology (Shanghai, China), and FBS was obtained from Sijiqing Biological Engineering Materials Co., Ltd (Hangzhou, China). Antibodies against TRIM32 were purchased from Bioss (China), NLRP3, NF-κB p65, phospho-NF-κB p65, cleaved caspase-1, ASC, IL-18, and IL-1β were purchased from Wanleibio (Shenyang, Liaoning, China), GSDMD-N and horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG antibodies were purchased from ABclonal (Wuhan, China).
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