Cellulose nitrate

Manufactured by Cytiva

Sourced in United Kingdom

Cellulose nitrate is a type of lab equipment used in various applications. It serves as a filtration medium, allowing the separation and isolation of substances from complex mixtures. The core function of cellulose nitrate is to facilitate the filtration process in a laboratory setting.

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Lab products found in correlation

Cellulose acetate, by Cytiva (1 mentions) Na2s 9h2o, by Merck Group (1 mentions) Nh4 2moo4, by Merck Group (1 mentions) Nh4 2mos4, by Merck Group (1 mentions)

5 protocols using cellulose nitrate

Microplastic Sampling Quality Control

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Exposure times during all stages of sample collection and processing
were limited. Samples were covered at all times with clean jar lids, aluminum
foil or glass eyewashes. All glassware and equipment used in this study were
cleaned with Decon-90, rinsed three times with tap water followed by a final
rinse using filtered water (0.45 μm cellulose nitrate, Whatman). In the
field and lab, only 100% cotton clothing and white lab coats were worn. All
samples were filtered inside a clean air cabinet and covered during filtration.
Filters were immediately placed in clean Petri dishes until after initial visual
inspection. Once all fibers/questionable particles were recorded, lids were
removed to access suspect particles for verification.
Two wet peroxide oxidation controls using filtered water (0.45 μm
cellulose nitrate, Whatman) were processed identical to our environmental
samples to estimate procedural contamination. In addition, air contamination
controls were conducted during lab processing and filtering of samples
(n = 4). Filters moistened with filtered water (0.45
μm cellulose nitrate, Whatman) were exposed to the air next to where
vacuum filtration occurred for 30 minutes.

Kapp K.J, & Yeatman E. (2018). Microplastic Hotspots in the Snake and Lower Columbia Rivers: A Journey from the Greater Yellowstone Ecosystem to the Pacific Ocean. Environmental pollution (Barking, Essex : 1987), 241, 1082-1090.

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Filtration System Comparison for Microparticle Analysis

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A complete filtration system of 25 mm (Product no. 073-0Q7724, Scharlab) was used for filtration. The filters assessed were polycarbonate cyclopore track etched (Catalogue no. 7060-2504, Whatman), nylon (Catalogue no. 7404-002, Whatman), cellulose acetate (Catalogue no. 10404006, Whatman), cellulose nitrate (Catalogue no. 10401106, Whatman), and mixed cellulose ester (Ref. HAWP02500, Merck Millipore Ltd), in all instances 0.45-μm pore size and 25-mm diameter. The magnetic actuation was achieved with the 16-tube magnet (Product no. 12321D, Thermo Fisher Scientific). The electrochemical readout was achieved on carbon screen-printed electrodes (ref. DRP-C110) using a portable bipotentiostat DRP-STAT200 operated by DropView 200 for instrument control and data acquisition (Dropsens, Spain). The scanning electron microscope (SEM) images were taken with the EVO MA-10 (with EDS Detector, Oxford LINCA).

Mesas Gómez M., Molina-Moya B., de Araujo Souza B., Boldrin Zanoni M.V., Julián E., Domínguez J, & Pividori M.I. (2024). Improved biosensing of Legionella by integrating filtration and immunomagnetic separation of the bacteria retained in filters. Mikrochimica Acta, 191(2), 82.

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Production of Bacterial and Fungal Biocontrol Agents

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Preparation of cultural filtrates of each bacterial biocontrol agent was carried out using sterilized nutrient glucose (2%) broth medium (3 g beef extract, 5 g peptone, 20 g glucose in one liter distilled water, pH 7.2) in 250 ml flasks. Each flask was separately inoculated with 1 ml of 48-h-old-culture of each bacterial agent. Three flasks were used as replicates for each bacterial antagonist. The inoculated flasks were incubated at 28 ± 2 °C for 48 h under static conditions. Each bacterial culture was centrifugated at 2038 × g for 15 min; then, the supernatant was filtered through filter paper (Whatman No.1) and finally sterilized by filtration through sterile 0.45 μm membrane filter (cellulose nitrate, Whatman). The bacterial filtrates were then kept at -20 °C until used (Abd-El-Khair and Haggag 2007) .
Preparation of cultural filtrates of each Trichoderma spp. was made using sterilized potato glucose 2% broth medium (200 ml potato extract and 20 g glucose in one liter distilled water) in 250 ml flasks. Each flask was separately inoculated with 1 cm-diameter disc of one-weekold culture of each Trichoderma spp. The inoculated

Abd-El-Khair H., Abdel-Gaied T.G., Mikhail M.S., Abdel-Alim A.I., & El-Nasr H.I.S. (2021). Biological control of Pectobacterium carotovorum subsp. carotovorum, the causal agent of bacterial soft rot in vegetables, in vitro and in vivo tests. Bulletin of the National Research Centre/Bulletin of the National Research Center, 45(1).

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Stable Molybdenum Sulfur Stock Solutions

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All stock solutions were prepared at the Environmental Geochemistry Group at the University of Bayreuth. A glovebox (COY, N2/H2 95/5% (v/v)) was used for preparation of solutions. Stock solutions of ammonium molybdate ((NH4)2MoO4, Sigma-Aldrich), ammonium tetrathiomolybdate ((NH4)2MoS4, Sigma-Aldrich), and sodium sulfide nonahydrate (Na2S•9H2O, Sigma-Aldrich) were prepared with N2-purged ultrapure water (Merck, Millipore, 18.2 mΩ•cm, 7 ppb total organic carbon at 25°C). The MoS4 2-stock solution was filtered (0.2 µm, celluloseacetate filter, Membrex or cellulose-nitrate, Whatmann) to remove insoluble particles, flash-frozen, and stored in liquid nitrogen immediately after preparation to ensure stability. 38 Aliquots were thawed directly before starting the experiments to minimize alteration. All pipette tips (Sarstedt) and screw cap vials (1.5 mL glass (VWR), 2 mL PP (Hartenstein), and 10 mL PP (Sarstedt)) were acidcleaned with 3 M HCl (Grüssing) for 24 h and rinsed with ultrapure water.

Kerl C.F., Lohmayer R., Bura-Nakić E., Vance D, & Planer-Friedrich B. (2017). Experimental Confirmation of Isotope Fractionation in Thiomolybdates Using Ion Chromatographic Separation and Detection by Multicollector ICPMS. Analytical chemistry, 89(5).

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Fungal Growth with Pb and P Sources

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A. niger and P. javanicus were chosen as test organisms for detailed experiments.
These were grown in MCD amended with 5 mM Pb(NO3)2 and 30 mM G2P or 5 mM PyA as sole phosphorus sources in 250-ml conical flasks containing 100 ml nutrient medium on an orbital shaking incubator at 125 rpm at 25°C in the dark. Pb(NO3)2, G2P and PyA were dissolved separately in Milli-Q water and sterilized by membrane filtration (cellulose nitrate, 0.2 µm pore diameter, Whatman, Maidstone, Kent, UK) and added to autoclaved MCD medium (121 o C, 15 min) at room temperature, to give 5 mM Pb(NO3)2, 30 mM G2P and 5 mM PyA final concentrations. All experiments were conducted at least in triplicate.

Liang X., Kierans M., Ceci A., Hillier S, & Gadd G.M. (2016). Phosphatase-mediated bioprecipitation of lead by soil fungi. Environmental microbiology, 18(1).

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