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Apc cy7 cd3 clone sp34 2 and v500 cd4 clone l200

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APC-Cy7 CD3 (Clone SP34–2) and V500 CD4 (clone L200) are fluorochrome-conjugated antibodies used for the identification and quantification of T lymphocytes in flow cytometry applications. APC-Cy7 CD3 binds to the CD3 surface antigen expressed on T cells, while V500 CD4 binds to the CD4 surface antigen expressed on helper T cells. These reagents provide a reliable and standardized method for the phenotypic analysis of T cell subsets.

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Lab products found in correlation

Af405 cd8, by BD (2 mentions) Foxp3 staining buffer set, by Thermo Fisher Scientific (2 mentions) Lsrii flow cytometer, by BD (2 mentions)

Close spelling variants of this product

CD3 APC-Cy7 (clone SP34-2; CD3 V500 (clone SP34-2, CD3-APC/Cy7 (SP34-2), CD3-APC (SP34–2, CD3 (clone SP34-2, CD4 APC (L200) CD4 (clone L200, Clone SP34-2 CD3-APC-Cy7 CD4-APC-Cy7

2 protocols using apc cy7 cd3 clone sp34 2 and v500 cd4 clone l200

1

Flow Cytometric Analysis of PBMCs

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Peripheral blood mononuclear cells (PBMCs) were isolated by Ficoll density gradient centrifugation and cryopreserved in liquid nitrogen until analysis. Immunophenotypic analysis was performed using the following directly conjugated anti-human antibodies: APC-Cy7 CD3 (Clone SP34–2) and V500 CD4 (clone L200) obtained from BD Biosciences; AF405-CD8 (Caltag; clone 3B5). Cell proliferation was monitored by staining with AF700- or FITC-Ki-67 Ab in cells permeabilized with the Foxp3 Staining Buffer Set (eBioscience). All the samples were acquired in a LSR II Flow Cytometer (BD) and analyzed using FlowJo software (Tree Star, Inc., Ashland, OR).
Bergamaschi C., Watson D.C., Valentin A., Bear J., Peer C.J., Figg WD S.r., Felber B.K, & Pavlakis G.N. (2018). Optimized administration of hetIL-15 expands lymphocytes and minimizes toxicity in rhesus macaques. Cytokine, 108, 213-224.
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2

Flow Cytometric Analysis of PBMCs

Check if the same lab product or an alternative is used in the 5 most similar protocols
Peripheral blood mononuclear cells (PBMCs) were isolated by Ficoll density gradient centrifugation and cryopreserved in liquid nitrogen until analysis. Immunophenotypic analysis was performed using the following directly conjugated anti-human antibodies: APC-Cy7 CD3 (Clone SP34–2) and V500 CD4 (clone L200) obtained from BD Biosciences; AF405-CD8 (Caltag; clone 3B5). Cell proliferation was monitored by staining with AF700- or FITC-Ki-67 Ab in cells permeabilized with the Foxp3 Staining Buffer Set (eBioscience). All the samples were acquired in a LSR II Flow Cytometer (BD) and analyzed using FlowJo software (Tree Star, Inc., Ashland, OR).
Bergamaschi C., Watson D.C., Valentin A., Bear J., Peer C.J., Figg WD S.r., Felber B.K, & Pavlakis G.N. (2018). Optimized administration of hetIL-15 expands lymphocytes and minimizes toxicity in rhesus macaques. Cytokine, 108, 213-224.
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