Total RNA was isolated with TRIzol (Invitrogen, USA) according to the manufacturer’s instructions. cDNA was synthesized with the PrimeScriptRT reagent Kit (Promega, USA). qRT-PCR was carried out using the SYBR Green SuperMix kit (Roche, Switzerland) and ABI 7900HT Fast Real-Time PCR system (Applied Biosystems). U6 was used as the internal control. The primers of miR-182-5p and U6 were purchased from Ribobio.
Primers of mir 182 5p and u6
Manufactured by RiboBio
Primers of miR-182-5p and U6 are laboratory reagents used in molecular biology techniques. The miR-182-5p primer is designed to target the miR-182-5p RNA molecule, while the U6 primer is designed to target the U6 small nuclear RNA. These primers are typically used in gene expression analysis and quantification methods, such as real-time PCR and reverse transcription-PCR.
Automatically generated - may contain errors
2 protocols using primers of mir 182 5p and u6
1
miRNA-182-5p Expression Analysis
2
Quantification of miR-182-5p expression
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Total RNA was isolated with TRIzol (Invitrogen, USA) according to the manufacturer's instructions. cDNA was synthesized with the PrimeScript RT reagent Kit (Promega, USA). qRT-PCR was carried out using the SYBR Green SuperMix kit (Roche, Switzerland) and ABI 7900HT Fast Real-Time PCR system (Applied Biosystems). U6 was used as the internal control. The primers of miR-182-5p and U6 were purchased from Ribobio.
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