Sirna oligos

Ad-KLF5 and Ad-GFP were made as described previously [13] . Hela cells were infected with Adenoviral vector (Genechem Co., Ltd). To achieve RNAi-mediated depletion of Eppk1, we transfected cells with siRNA oligos targeting Eppk1 or negative controls (Genechem Co., Ltd). In this process, we also used the Lipofectamine 2000 reagents (Invitrogen; Thermo Fisher Scienti c, Inc.) according to the operation instructions.
Immuno uorescence staining.
All cervical tissue samples were xed by 4% paraformaldehyde solution, the xed tissue was dehydrated by ethanol, transparent by xylene, embedded in para n, and the sections were 4 μm. The above samples were operated by indirect immuno uorescence method according to the instructions. rabbit anti-KLF5
(1:50, GTX103289, GeneTex) and mouse anti-Eppk1 (1:50, sc-87102, Santa) were incubated overnight at 4℃, Fluorescein labeled uorescent secondary antibody, Rhodamine labeled uorescent secondary antibody (KPL) and DAPI (Sigma) nuclear staining. Image Pro-Plus6.0 (Media Cybernetics, Inc, USA) Image analysis software analyzed the uorescence intensity of Eppk1 and KLF5 expressions.

Ad-KLF5 and Ad-GFP were made as described previously [13] . Hela cells were infected with Adenoviral vector (Genechem Co., Ltd). To achieve RNAi-mediated depletion of Eppk1, we transfected cells with siRNA oligos targeting Eppk1 or negative controls (Genechem Co., Ltd). In this process, we also used the Lipofectamine 2000 reagents (Invitrogen; Thermo Fisher Scienti c, Inc.) according to the operation instructions.