Two human MPM cell lines, EHMES-10 and MSTO-211H, were used in this study. EHMES-10 cells were established from the pleural effusion of a patient with MPM at Ehime University (Matsuyama, Japan) [23, 24] and MSTO-211H cells were purchased from the American Type Culture Collection (Manassas, VA, USA). BALB/c mouse primary pulmonary artery endothelial cells were purchased from Cell Biologics (Chicago, IL, USA). MPM cell lines were cultured in RPMI 1640 medium (Gibco, Grand Island, NY, USA) supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin. Mouse endothelial cells were cultured in Mouse Endothelial Cell Medium /w (Cell Biologics). All cells were cultured at 37°C in a 5% CO 2 humidified atmosphere. MSTO-211H and mouse artery endothelial cells were used within 6 months after thawing, and EHMES-10 cells were authenticated by short tandem repeat analyses (Promega, Tokyo, Japan).
Ehmes 10
Manufactured by American Type Culture Collection
Sourced in United States
The EHMES-10 is a laboratory equipment designed for efficient homogenization and emulsification of samples. It features a high-speed motor and a specialized head for versatile processing of a wide range of materials. The core function of the EHMES-10 is to effectively disrupt and mix samples to produce a uniform, well-dispersed mixture.
Automatically generated - may contain errors
Lab products found in correlation
Msto 211h, by American Type Culture Collection (2 mentions)
Nci h28, by American Type Culture Collection (2 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Msto 211h cells, by American Type Culture Collection (1 mentions)
Topotecan, by Merck Group (1 mentions)
Clodronate, by Merck Group (1 mentions)
Ggti 298, by Merck Group (1 mentions)
C3 transferase, by Cytoskeleton (1 mentions)
Lipofectamine rnaimax, by Thermo Fisher Scientific (1 mentions)
Nci h2452, by American Type Culture Collection (1 mentions)
Nci h226, by American Type Culture Collection (1 mentions)
3 protocols using ehmes 10
1
Establishing and Characterizing MPM Cell Lines
2
Comprehensive Mesothelioma Cell Line Protocol
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Human mesothelioma, MSTO-211H and NCI-H28 cells, were purchased from American Type Culture Collection (Manassas, VA, USA), and EHMES-1, EHMES-10 and JMN-1B cells were provided by Dr Hamada (Ehime University, Ehime, Japan). ZOL were purchased from Novartis (Basel, Switzerland), and FOH, GGOH, GGTI-298, ETO, topotecan and clodronate were from Sigma-Aldrich (St Louis, MO, USA). C3 transferase and NSC23766 were from Cytoskeleton (Denver, CO, USA) and Merck Millipore (Billerica, MA, USA), respectively. NE10790 were provided by Dr Ebetino FH (Warner Chilcott, Dundalk, Ireland). We purchased siRNA duplex targeting Cdc42 and non-specific control siRNA from Invitrogen (Carlsbad, CA, USA). Transfection of cells with the siRNA was conducted with Lipofectamine RNAiMAX (Invitrogen).
3
Mesothelioma Cell Line Characterization
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Human mesothelioma cells, MSTO-211H, NCI-H28, NCI-H226, NCI-H2452 cells, all of which were purchased from American Type Culture Collection (Manassas, VA, USA), and EHMES-10 (provided from Dr. Hironobu Hamada, Hiroshima University, Japan) [18 (link)] and were cultured with RPMI 1640 medium with 10 % fetal calf serum. HEK 293 and A549 cells, derived from American Type Culture Collection and Dr. Katsuyuki Hamada (Ehime University), respectively, were cultured with in Dulbecco’s Modified Eagle’s Medium containing 10 % fetal calf serum. NCI-H28, NCI-H2452 and EHMES-10 cells are defective of the p14ARF and p16INK4A genes, and MSTO-211H and NCI-H226 cells lack the p14 and p16 transcription (Additional file 1 : Figure S1). Sequence analyses showed that all of them possessed the wild-type p53 gene.
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