ACGs were prepared in Huang’s laboratory (Institute of Medicinal Plant Development [IMPLAD], Beijing, China) and the composition is shown in Table S1 (batch number 091). Mono-(6-ethanediamine-6-deoxy)-beta-CD (NH2-β-CD) was purchased from Zhiyuan Biotechnology Co Ltd, Shandong, China. FA was obtained from Sinopharm Chemical Reagent Beijing Co, Ltd (Beijing, China). Dicyclohexylcarbodiimide (DCC) and 1-hydroxy-2,5-pyrrolidinedione (NHS) were purchased from Sinopharm Chemical Reagent Beijing Co, Ltd. SPC was obtained from Guangzhou Hanfang Pharmaceutical Co Ltd (Guangdong, China). The Float-A-Lyzer® G2 dialysis cassette was obtained from Spectrum Labs (Rancho Dominguez, CA, USA). The microplate test kit was purchased from Nanjing JianCheng Bioengineering Institute (Nanjing, China). Cy5.5 (a fluorescent probe) and 1,1′-dioctadecyltetramethyl indotricarbocyanine iodide (DiR) were obtained from AAT Bioquest Inc. (Sunnyvale, CA, USA). PTX injections were supplied by Beijing Union Pharm Ltd (Beijing, China). All other reagents were of analytical grade or higher.
Dicyclohexylcarbodiimide dcc
Manufactured by Sinopharm
Sourced in China
Dicyclohexylcarbodiimide (DCC) is a chemical reagent used in organic synthesis for the formation of amide bonds. It is a white, crystalline solid that acts as a dehydrating agent, facilitating the coupling of carboxylic acids and amines to produce peptides and other amide-containing compounds.
Automatically generated - may contain errors
Lab products found in correlation
Dspe peg2000 mal, by NOF corporation (3 mentions)
4 dimethylaminopyridine dmap, by Sinopharm (2 mentions)
Microplate test kit, by Nanjing Jiancheng (1 mentions)
Trypsin, by Macgene (1 mentions)
Tunel kit, by Keygen Biotech (1 mentions)
Solutol hs 15, by BASF (1 mentions)
Cell counting kit 8 cck 8, by Solarbio (1 mentions)
Penicillin streptomycin, by Macgene (1 mentions)
Hoechst 33258, by Macgene (1 mentions)
N n dimethylacrylamide, by J&K Scientific (1 mentions)
3 protocols using dicyclohexylcarbodiimide dcc
1
Functionalized Nanoparticle Drug Delivery
2
Peptide-Functionalized Paclitaxel-Loaded Liposomes
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Distearoyl-glycerophosphoethanolamine-polyethyleneglycol2000-maleimide (DSPE-PEG2000-Mal) was purchased from NOF Corporation (Tokyo, Japan). Peptide (CYKLEGTTRLTRKRGLKLA) was custom synthesized by GL Biochem (Shanghai, China). Paclitaxel (PTX) was purchased from Norzer Pharmaceutical Co., Ltd. Alpha linolenic acid (ALA) was purchased from ANPEL Laboratory Technologies (Shanghai, China). Soybean phosphatidylcholine (SPC), unesterified cholesterol (UC), triolein (TO) and cholesteryl oleate (CO) were from Lipoid (Ludwigshafen, Germany), Wako Ltd. (Tokyo, Japan), Sinopharm Chemical Reagent Co., Ltd (Beijing, China) and InnoChem Science & Technology Co., Ltd. (Beijing, China), respectively. Solutol® HS 15 was produced by BASF (Germany). Dicyclohexylcarbodiimide (DCC) and 4-dimethylaminopyridine (DMAP) were obtained from Sinopharm Group Co., Ltd. Cell Counting Kit-8 (CCK- 8) was obtained from SolarbioScience & Technology Co., Ltd (Beijing, China). Coumarin-6 (COU) was purchased from Sigma-Aldrich Company (USA). TUNEL Kit was obtained from KeyGEN Biotech Co., Ltd. (Nanjing, China). Enzyme-linked Immunosorbent Assay (ELISA) Kit for LDLR was obtained from USCN Life Science Inc. Modified eagle medium (MEM), DMEM, penicillin-streptomycin, trypsin and Hoechst 33258 were obtained from Macgene Technology (Beijing, China).
3
Synthesis of Pyrene-Labeled Cyclodextrin Polymers
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N,N-dimethylacrylamide (DMAA, 99%), α-cyclodextrins (α-CD, 98%), β-cyclodextrins (β-CD, 98%), γ-cyclodextrins (γ-CD, 98%) and 1-pyrenebutyric acid (PyBA, 97%) were purchased from J&K Scientific Co., Ltd. (Shanghai, China). Azobisisobutyronitrile (AIBN) was purchased from Tianjin Bodi Chemical Co., Ltd. (Tianjin, China) and purified by recrystallization from ethanol. 2-Hydroxyethyl methacrylate (HEMA, 99%) was purchased from Shanghai Aladdin Chemical Reagent Co., Ltd. (Shanghai, China). Dicyclohexylcarbodiimide (DCC) and 4-dimethylaminopyridine (DMAP) were purchased from Sinopharm Chemical Reagent Co., Ltd. (Beijing, China). Tetrahydrofuran (THF), dichloromethane, diethyl ether and ethanol were used as received from commercial sources. All other reagents were used without further purification.
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