Agilent g9800a fluorescence spectrophotometer

The chemistry reagents (chemical grade) used in experiments was obtained from Sino Pharm Chemical Reagent Co. Ltd. (Shanghai, China). The real samples river water and tap water were obtained from the Shanghai University of Traditional Chinese Medicine, and sugar was purchased from a local supermarket (Shanghai, China). The chrysanthemum was purchased from Shanghai Kangqiao Chinese Medicine, grown in HangZhou, China. The analytical thin-layer chromatography (TLC) with pre-coated silica gel GF254 plates (Sino Pharm Chemical Reagent Co. Ltd., Shanghai, China) was used to detect any spots at 254 nm under UV light. ¹H-NMR and ¹³C-NMR spectra were measured at 25 °C and referenced to tetramethyl silane (TMS) by a BRUKER AVANCE III spectrometer (Bruker, German). Electron ionization mass spectra (ESI-MS) was recorded on an Agilent 6460 triple quad LC-MS mass spectrometer (Agilent, Santa Clara, CA, USA). UV-Vis spectra and fluorescence spectra were recorded by Agilent 8454 UV-Vis spectrometer (Agilent) and Agilent G9800A fluorescence spectrophotometer (Agilent) respectively. Leica TCS-SP8 multiphoton illustrated the fluorescence images with the confocal microscope having a 63 × oil-immersion objective lens (Leica, Wetzlar, German).

All chemicals (reagent grade) used were purchased from Sino Pharm Chemical Reagent Co., Ltd. (Shanghai, China). Reaction progress was monitored using analytical thin layer chromatography (TLC) on pre-coated silica gel GF254 (Qingdao Haiyang Chemical Plant, Qingdao, China) plates, and spots were detected under UV light (254 nm). Melting point was measured on an XT-4 micromelting point instrument and uncorrected. IR (KBr-disc) spectra were recorded by a Bruker Tensor 27 spectrometer (Bruker, German). ¹H-NMR and ¹³C-NMR spectra were measured on a BRUKER AVANCE III spectrometer at 25 °C and referenced to tetramethyl silane (TMS) (Bruker, German). Mass spectra were obtained on an MS Agilent 1100 Series LC/MSD Trap mass spectrometer (Agilent, Santa Clara, CA, USA). UV-vis spectra were recorded on an Agilent 8454 UV-vis spectrometer (Agilent). Fluorescence spectra measurements were recorded on an Agilent G9800A fluorescence spectrophotometer (Agilent). Fluorescence images were obtained on a Leica TCS-SP8 multiphoton; a confocal microscope and a 63× oil-immersion objective lens was used (Leica, Switzerland). High-resolution EI mass spectra were recorded on an Agilent 6460 triple quad LC-MS mass spectrometer (Agilent).