Athymic NCR nu/nu nude mice were obtained from Taconic Farms Inc., USA. According to the supplier, the autosomal recessive nude gene in homozygous (nu/nu) mice caused the lack of fur and an abnormal thymus. The deficiency in T cell function allowed athymic mice to accept and develop xenografts tumor model. The mice of the same gender were housed in specific pathogen free (SPF) cages supplied with high efficiency particulate air (HEPA) filters. Free access to autoclaved food and water was provided and the autoclaved bedding was changed twice weekly. The procedures were approved by the USM Animal Ethics Committee with the reference number USM/2014/ (94) (672).
Ncr nu nu athymic nude mice
Manufactured by Taconic Biosciences
Sourced in United States
NCr nu/nu athymic nude mice are a laboratory mouse model characterized by a genetic mutation that results in a lack of functional thymus gland and impaired immune system. These mice are widely used in biomedical research due to their reduced immune response, which allows for the study of human tumor xenografts and other applications that require an immunocompromised host.
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5 protocols using ncr nu nu athymic nude mice
1
Athymic Nude Mice for Xenograft Tumor Models
2
Athymic Nude Mice Housing Protocol
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Athymic NCR nu/nu nude mice aged 6-8 weeks with a weight of 25 ± 2g were obtained from Taconic Farms Inc., USA. The mice were kept in a specific pathogen free (SPF) cages provided with high efficiency particulate air (HEPA) filter using Animal
Transport Unit (Allenton.USA). Mice were provided with sterile food, water and bedding using autoclave (Hirayama-Hiclave, Japan) and housed under a standardized 12 h light and 12 h dark cycle at room temperature of 24 ± 2°C and a humidity of 60%.
Experimental work was done according to the guidelines of Universiti Sains Malaysia (USM) Animal Ethics Committee (reference number: USM/ Animal Ethics Approval (2 013 / (89) (492).
Transport Unit (Allenton.USA). Mice were provided with sterile food, water and bedding using autoclave (Hirayama-Hiclave, Japan) and housed under a standardized 12 h light and 12 h dark cycle at room temperature of 24 ± 2°C and a humidity of 60%.
Experimental work was done according to the guidelines of Universiti Sains Malaysia (USM) Animal Ethics Committee (reference number: USM/ Animal Ethics Approval (2 013 / (89) (492).
3
Xenograft Tumor Growth Inhibition
4
Orthotopic Xenograft Model of Breast Cancer
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Five-week-old ovariectomized NCr nu/nu athymic nude mice (Taconic Biosciences, Rensselaer, NY) were injected orthotopically with 1.0 × 106 LCC1/LCC9 cells in 50% Matrigel into mammary fat pads. There were two tumors per mouse and five mice per treatment for each cell line that resulted in ten tumors per treatment group. Treatments were: vehicle alone (for CB-839, 25% hydroxypropyl-β-cyclodextrin in 10 mM citrate, pH 2 (27 (link)), or for everolimus, 30% propylene glycol and 5% Tween 80), CB-839 (200 mg/kg by oral gavage twice daily), everolimus (5 mg/kg by intraperitoneal, IP, one injection daily) or the combination of CB-839 and everolimus. Body weight and tumor size were monitored weekly. For all groups, treatment began on day-14 post-inoculation and continued for 3 weeks. All mice were sacrificed at day-35 post-inoculation and tumors were collected for further analysis. Mice were housed and maintained under specific pathogen-free conditions and used in accordance with institutional guidelines approved by Georgetown University Animal Care and Use Committee (GUACUC; protocol #2016-1250).
5
Xenograft Model of Ovarian Cancer
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All animal experiments were performed in accordance with the National Institutes of Health Guidelines for the Care and Use of Laboratory Animals and the established Institutional Animal Use and Care protocol at the University of Illinois at Chicago (UIC). In addition, the Animal Care Committee approved the protocol 11–066. Female NCr nu/nu athymic (nude) mice were obtained from Taconic (Hudson, NY, USA). Mice were housed in a temperature and light controlled environment (12 h light, 12 h dark) and provided food and water ad libitum.
The procedure of injections was performed as previously described [41 (link)]. Briefly, i.p. (1 × 107 cells in PBS/animal) injections with MOSE-Neo or MOSE-PAX8 cells were performed on all mice (n = 6 mice/group). After 6 months, mice were euthanized by CO2 asphyxiation. Gross internal anatomy was inspected for tumors.
The procedure of injections was performed as previously described [41 (link)]. Briefly, i.p. (1 × 107 cells in PBS/animal) injections with MOSE-Neo or MOSE-PAX8 cells were performed on all mice (n = 6 mice/group). After 6 months, mice were euthanized by CO2 asphyxiation. Gross internal anatomy was inspected for tumors.
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