2 5 dihydroxybenzioc acid dhb

Manufactured by Merck Group

2,5-Dihydroxybenzioc Acid (DHB) is a chemical compound that serves as a laboratory reagent. It is commonly used as a matrix in matrix-assisted laser desorption/ionization (MALDI) mass spectrometry, a technique for the analysis of biomolecules such as proteins, peptides, and oligonucleotides.

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Lab products found in correlation

Sinapinic acid, by Merck Group (1 mentions) Ethanol, by Thermo Fisher Scientific (1 mentions) Hplc grade methanol, by Thermo Fisher Scientific (1 mentions) Trifluoroacetic acid, by Merck Group (1 mentions) Hplc grade methanol meoh, by Thermo Fisher Scientific (1 mentions) Indium tin oxide slides, by Bruker (1 mentions) Trifluoroacetic acid tfa, by Merck Group (1 mentions)

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2,5-DHB (6 citations)

2 protocols using 2 5 dihydroxybenzioc acid dhb

MALDI-IMS Sample Preparation Protocol

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Sinapinic acid, 2,5-Dihydroxybenzioc Acid (DHB) and trifluoroacetic acid were obtained from Sigma-Aldrich (St. Louis, MO). HPLC-grade m ethanol, ethanol and water were obtained from Fisher Scientific. Immobilized titanium oxide (ITO) slides were purchased from Bruker Daltonics (Billerica, MA) for MALDI-IMS experiments. Mass spectrometry-grade trypsin gold was from Promega (Madison, WI, USA).

Jones E.E., Powers T.W., Neely B.A., Cazares L.H., Troyer D.A., Parker A.S, & Drake R.R. (2014). MALDI Imaging Mass Spectrometry Profiling of Proteins and Lipids in Clear Cell Renal Cell Carcinoma. Proteomics, 14(0), 924-935.

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Lipid Standards and MALDI-IMS Analysis

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Ceramide
lipid standards were purchased from
Avanti-Polar Lipids Inc. (Alabaster, AL). 2,5-Dihydroxybenzioc acid
(DHB) and trifluoroacetic acid (TFA) were obtained from Sigma-Aldrich
(St. Louis, MO). HPLC-grade methanol (MeOH), ethanol, and water were
obtained from Fisher Scientific. Indium tin oxide (ITO) slides were
purchased from Bruker for MALDI-IMS experiments. Recombinant bCDase
and bSMase were expressed and purified as previously described.^{20 (link)}

Jones E.E., Dworski S., Canals D., Casas J., Fabrias G., Schoenling D., Levade T., Denlinger C., Hannun Y.A., Medin J.A, & Drake R.R. (2014). On-Tissue Localization of Ceramides and Other Sphingolipids by MALDI Mass Spectrometry Imaging. Analytical Chemistry, 86(16), 8303-8311.

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