Using PBS bufffer to collect and wash cell suspension (1 × 106 cells/ml) twice. Cells were stained with Huh7 PE (BD, USA), Huh7 7-ADD (BD, USA), Huh7 PE and 7-ADD together, PE and 7-ADD together. A negative control was processed in parallel. Then, measured cell apoptosis by FACSCanto II flow cytometer (Beckman, USA).
+ Open protocol