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Lyophilized fmoc ff powder

Manufactured by Bachem
Sourced in Switzerland

Lyophilized Fmoc-FF powder is a laboratory product manufactured by Bachem. It is a solid, white to off-white powder that is produced through the process of lyophilization. The product's core function is to serve as a component in further chemical synthesis and research applications.

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3 protocols using lyophilized fmoc ff powder

1

Fmoc-FF Peptide Characterization Protocol

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Lyophilized Fmoc-FF powder was purchased from Bachem (Bubendorf, Switzerland). TWEEN® 60, SPAN® 60, oil mineral, and all other chemicals were purchased from Sigma-Aldrich, Fluka (Bucks, Switzerland) or LabScan (Stillorgan, Dublin, Ireland) and were used as received unless otherwise stated. All solutions were obtained by weight using doubly distilled water as a solvent. The effective peptide concentrations in solution were spectroscopically determined by UV-Vis measurements on a nanodrop 2000c spectrophotometer (Thermo Fisher Scientific Inc., Wilmington, DE, USA) equipped with a 1.0 cm quartz cuvette (Hellma) using as molar absorptivity (ε) the values of 7800 mol−1 L cm−1 at 301 nm.
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2

Synthesis and Characterization of Pegylated Liposomal Doxorubicin

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Protected Nα-Fmoc-amino acid derivatives, coupling reagents, and Rink amide MBHA (4-methylbenzhydrylamine) resin were purchased from Calbiochem-Novabiochem (Laufelfingen, Switzerland). The monodisperse Fmoc-8-amino-3,6-dioxaoctanoic acid (Fmoc- AdOO-OH, PEG2) was purchased from Neosystem (Strasbourg, France). Lyophilized Fmoc-FF powder was purchased from Bachem (Bubendorf, Switzerland). Doxorubicin chlorohydrate (Dox.HCl) was purchased from Sigma Aldrich (Milan, Italy). Pegylated liposomal Dox (commercial name of Doxil) vials were kindly gifted by the Italian Cancer Institute in Naples (Italy) “Fondazione G. Pascale”. TWEEN®60, SPAN®60, and all other chemicals and solvents were purchased from Sigma-Aldrich, Fluka (Bucks, Switzerland) or LabScan (Stillorgan, Dublin, Ireland) and were used as received unless otherwise stated. All solutions were obtained by weight using doubly distilled water as a solvent. UV-Vis spectra were recorded on a Thermo Fisher Scientific Inc (Wilmington, Delaware USA) Nanodrop 2000c, equipped with a 1.0 cm quartz cuvette (Hellma).
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3

Fabrication of Fmoc-FF Nanoparticles

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Lyophilized Fmoc-FF powder was purchased from Bachem (Bubendorf, Switzerland). Dexamethasone (DEX) was purchased from Sigma Aldrich (Milan, Italy). TWEEN®60, SPAN®60, and all other chemicals and solvents were obtained from commercial sources and used as received unless otherwise stated. All the solutions were prepared by weight using doubly distilled water.
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