Subjects were male R6/1 (B6.Cg-Tg (HDexon1) 61Gpb/J, Stock number: 006471, Jackson Laboratory, Main Harbor, NY, USA) and age-matched wild-type (WT) littermates [16 (link)] which were bred and genotyped as previously described [17 (link),18 (link)]. While the CAG repeat size was not determined for the mice used in this experiment, the number in the breeders was 123.64 ± 0.89. Mice weaned at 21 days of age were group-housed with their same-sex littermates (3–5/cage) until the beginning of experiments. NMRI female mice (Janvier, Le Genest-Saint-Isle, France) of 3–4 months of age were used as stimulus animals in the social tests. Each stimulus animal was used 3–4 times in the same experiment; its use and order of presentation was always balanced across genotypes. All animals were housed in unisexual groups (3–5 per cage) in polycarbonate standard cages (33x15x14 cm in size; Tecniplast, Limonest, France), provided with sawdust bedding (SAFE, Augy, France), and a stainless steel wire lid. Food chow (SAFE, Augy, France) and water were provided ad libitum. The animals were maintained in separate male and female identical colony rooms under temperature (22°C) and humidity-controlled (55%) conditions with a 12:12 hr light—dark cycle (lights on at 7 a.m.).
Polycarbonate standard cages
Manufactured by Tecniplast
Sourced in France
Polycarbonate standard cages are durable, transparent enclosures designed for housing small laboratory animals. They provide a secure and well-ventilated environment for the animals.
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3 protocols using polycarbonate standard cages
1
Huntington's Disease Mouse Model Breeding and Housing
2
Transgenic Huntington's Disease Mouse Model
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Experiments were performed on male wild-type (WT) (n = 41) and R6/1 transgenic littermate (n = 46) mice of 3 different ages: 2, 4 and 6 months representing, respectively, pre- symptomatic, early motor symptomatic and late symptomatic stages (Table 1 ). The mice were bred in the animal facility of Bordeaux University and were obtained from a parent genitor R6/1 mouse (B6.Cg-Tg(HDexon1)61Gpb/J, Stock number: 006471, Jackson Laboratory, Main Harbor, NY, USA) crossed with female C57BL/6 mice (IFFA/Credo, Lyon, France). The R6/1 line expresses exon 1 of the human HD gene with an expanded number of CAG trinucleotide repeats (123.64 ± 0.89). Genotypes were tested by PCR of tail biopsy specimens. All animals were housed in polycarbonate standard cages (33 x 15 x 14 cm in size; Tecniplast, Limonest, France), provided with sawdust bedding (SAFE, Augy, France) and a stainless steel wire lid. Food chow (SAFE, Augy, France) and water were provided ad libitum until experiment began. The animals were maintained in a colony room under temperature (22°C) and humidity-controlled (55%) conditions with a 12:12 hr light–dark cycle (lights on at 7 a.m.).
3
Behavioral Characterization of R6/1 Transgenic Mice
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Subjects were male and female R6/1 (B6.Cg-Tg (HDexon1) 61Gpb/J, Stock number: 006471, Jackson Laboratory, Main Harbor, NY, USA) and age-matched wild-type (WT) littermates. The R6/1 transgenic mouse lines express a transgene encoding the 5' end of human HTT (Mangriani, 996). While the CAG repeat size was not determined for the mice used in this experiment, the number in the breeders was 142.188 (± 1.195) . Mice came from 16 different litters including 2 to 7 littermates of both sexes.
PCR was performed on tail biopsy at weaning, and reconfirmed upon sacrifice for verification. [3] [4] month old NMRI female mice (Janvier, Le Genest-Saint-Isle, France) were used as stimulus animals in the social test. All animals were housed in unisexual groups (3-5 per cage) in polycarbonate standard cages (33x15x14 cm in size; Tecniplast, Limonest, France), provided with sawdust bedding (SAFE, Augy, France), and a stainless steel wire lid. Food chow (SAFE, Augy, France) and water were provided ad libitum unless otherwise mentioned. The animals were maintained in separate male and female colony rooms with temperature (22°C) and humidity (50%) being controlled. Mean ages were 85.80 ± 1.76 days for WT mice (n=24; 15 males and 9 females) and 87.67± 3.00 days for Tg mice (n=38; 21 males and 17 females) at the beginning of the experiment.
PCR was performed on tail biopsy at weaning, and reconfirmed upon sacrifice for verification. [3] [4] month old NMRI female mice (Janvier, Le Genest-Saint-Isle, France) were used as stimulus animals in the social test. All animals were housed in unisexual groups (3-5 per cage) in polycarbonate standard cages (33x15x14 cm in size; Tecniplast, Limonest, France), provided with sawdust bedding (SAFE, Augy, France), and a stainless steel wire lid. Food chow (SAFE, Augy, France) and water were provided ad libitum unless otherwise mentioned. The animals were maintained in separate male and female colony rooms with temperature (22°C) and humidity (50%) being controlled. Mean ages were 85.80 ± 1.76 days for WT mice (n=24; 15 males and 9 females) and 87.67± 3.00 days for Tg mice (n=38; 21 males and 17 females) at the beginning of the experiment.
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