Pp 229 aa

Manufactured by Biocare Medical

Sourced in United States

The PP 229 AA is a laboratory equipment product designed for analytical applications. It features automated functionalities to assist with sample processing and analysis. The core function of the PP 229 AA is to provide accurate and reliable data to support various scientific investigations, though its specific intended use is not included in this factual description.

Automatically generated - may contain errors

Lab products found in correlation

Benchmark ultra, by Roche (1 mentions) A0080, by Agilent Technologies (1 mentions) Ventana discovery ultra, by Roche (1 mentions) Hematoxylin 2, by Roche (1 mentions) Tris based reaction buffer, by Roche (1 mentions)

2 protocols using pp 229 aa

Immunohistochemical Analysis of Lung Autopsy

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Conventional hematoxylin and eosin (H&E) staining was performed on deparaffinized sections of the FFPE autopsy asseverates of the lungs according to the current accredited staining protocol at the Institute of Medical Genetics and Pathology of the University Hospital Basel, Switzerland, as per May 2020. Immunohistochemistry was performed according to the current accredited staining protocols, applying the polyclonal ready‐to‐use antibody PP 229 AA from Biocare (Pacheco, CA, USA) against cleaved caspase‐3 on an automated immunostainer Benchmark Ultra (Roche/Ventana, Tucson, AZ, USA), and the polyclonal antibody A0080 from Dako (Glostrup, Denmark) against fibrin(‐ogen) at a dilution of 1:100,000 utilizing detection with a secondary anti‐rabbit link antibody.

Yekelchyk M., Madan E., Wilhelm J., Short K.R., Palma A.M., Liao L., Camacho D., Nkadori E., Winters M.T., Rice E.S., Rolim I., Cruz‐Duarte R., Pelham C.J., Nagane M., Gupta K., Chaudhary S., Braun T., Pillappa R., Parker M.S., Menter T., Matter M., Haslbauer J.D., Tolnay M., Galior K.D., Matkwoskyj K.A., McGregor S.M., Muller L.K., Rakha E.A., Lopez‐Beltran A., Drapkin R., Ackermann M., Fisher P.B., Grossman S.R., Godwin A.K., Kulasinghe A., Martinez I., Marsh C.B., Tang B., Wicha M.S., Won K.J., Tzankov A., Moreno E, & Gogna R. (2021). Flower lose, a cell fitness marker, predicts COVID‐19 prognosis. EMBO Molecular Medicine, 13(11), e13714.

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Quantitative IHC Staining of Ki67 and Cleaved Caspase-3

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For Ki67 IHC staining formalin fixed-paraffin embedded tumor tissues, antigen retrieval was performed on the Roche Ventana Discovery ULTRA staining platform using Discovery CCI (Roche, Switzerland) for 36 minutes. Primary immunostaining was performed using a predilute Ki-67(Roche 790–4286) and incubated at 41°C for 32 minutes. Secondary immunostaining used a Horseradish Peroxidase (HRP) multimer cocktail (Roche) and immune complexes were visualized using the ultraView Universal DAB (diaminobenzidine tetrahydrochloride) Detection Kit (Roche). Slides were then washed with a Tris based reaction buffer (Roche) and counter-stained with Hematoxylin II (Roche cat) for 4 minutes. For cleaved-caspase-3 IHC staining, a similar protocol was followed using a Caspase-3-specific antibody (Biocare, PP229AA). For both IHC stains, analyses of positive tumor tissue from 5 randomly selected fields per tumor were done via ImageJ thresholding analysis.

Haber A.O., Jain A., Mani C., Nevler A., Agostini L.C., Golan T., Palle K., Yeo C.J., Gmeiner W.H, & Brody J.R. (2021). AraC-FdUMP[10] (CF10) is a next generation fluoropyrimidine with potent antitumor activity in PDAC and synergy with PARG inhibition. Molecular cancer research : MCR, 19(4), 565-572.

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