Dna purification and plasmid isolation kits

Strain P. oxalicum sp. 68 was isolated from soil in Changbai Mountain (Jilin Province, China), and stored in China General Microbiological Culture Collection Center (collection number CGMCC 7.328) [27 (link)]. P. pastoris GS115 and pPICZα A (Novagen, USA) were used as host and expression vectors, respectively. LWAG, debranched arabinan, arabinan (sugar beet), wheat arabinoxylan, galactomannan, and galactan (potato) were from Megazyme International Ireland Ltd. (Ireland). DNA purification and plasmid isolation kits were from Tiangen Biotech (China). All other chemicals and reagents were of analytical grade.

Propagation, subcloning, and storage of plasmids were performed within E. coli DH5α purchased from TransGen (China). Recombinant proteins were expressed in E. coli BL21(DE3) purchased from TransGen (China). E. coli MG1655 purchased from ZOMANBIO (China) were used for information storage and rewriting. DNA sequences including info plasmid, new information sequences, and the template for GFP expression (705 bp) were synthesized by GENEWIZ (China) and are listed in table S1. The strains used in this study are listed in table S2. Primers in table S3 were synthesized by Tsingke (China). After DNA amplification, procedures including DNA cloning, DNA fragment fusion, and polymerase chain reaction (PCR) test were performed to construct the donor DNA fragment, which used the enzymes purchased from Vazyme (China). DNA purification and plasmid isolation kits were purchased from TIANGEN (China).