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Slc30a4

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SLC30A4 is a zinc transporter protein that plays a role in the regulation of zinc homeostasis within cells. It is responsible for the transport of zinc ions across cell membranes. The core function of SLC30A4 is to facilitate the movement of zinc ions in and out of cells, contributing to the maintenance of appropriate zinc levels for various cellular processes.

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Lab products found in correlation

Lsm 710 confocal microscope, by Zeiss (6 mentions) Lamp1, by Abcam (2 mentions) Millicell four well glass ez slides, by Merck Group (2 mentions) Zinpyr 1, by Santa Cruz Biotechnology (2 mentions)

2 protocols using slc30a4

1

Confocal Imaging of Labile Zinc

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For confocal imaging, macrophages were plated on Millicell four-well glass EZ slides (Millipore) and treated as described above. Labile Zn was imaged 24 hr post-infection using Zinpyr-1 (Santa Cruz). Where indicated, cells were probed with SLC30A4 (Sigma) and LAMP1 (Abcam) antibodies. Images were acquired on a Zeiss LSM710 confocal microscope and visualized using ZEN 2011 software. MFI was analyzed using ImageJ.
Vignesh K.S., Landero Figueroa J.A., Porollo A., Divanovic S., Caruso J.A, & Deepe GS J.r. (2016). IL-4 Induces Metallothionein 3- and SLC30A4-Dependent Increase in Intracellular Zn2+ that Promotes Pathogen Persistence in Macrophages. Cell reports, 16(12), 3232-3246.
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2

Confocal Imaging of Labile Zinc

Check if the same lab product or an alternative is used in the 5 most similar protocols
For confocal imaging, macrophages were plated on Millicell four-well glass EZ slides (Millipore) and treated as described above. Labile Zn was imaged 24 hr post-infection using Zinpyr-1 (Santa Cruz). Where indicated, cells were probed with SLC30A4 (Sigma) and LAMP1 (Abcam) antibodies. Images were acquired on a Zeiss LSM710 confocal microscope and visualized using ZEN 2011 software. MFI was analyzed using ImageJ.
Vignesh K.S., Landero Figueroa J.A., Porollo A., Divanovic S., Caruso J.A, & Deepe GS J.r. (2016). IL-4 Induces Metallothionein 3- and SLC30A4-Dependent Increase in Intracellular Zn2+ that Promotes Pathogen Persistence in Macrophages. Cell reports, 16(12), 3232-3246.
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