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Bio plex pro human cytokine 21 plex and 27 plex assay

Manufactured by Bio-Rad

The Bio-Plex Pro™ Human Cytokine 21-plex and 27-plex Assays are multiplex immunoassays designed to detect and quantify multiple cytokines, chemokines, and growth factors simultaneously in a single well. These assays utilize magnetic color-coded beads and offer a convenient solution for researchers to measure the levels of various analytes in biological samples, such as cell culture supernatants, serum, and plasma.

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2 protocols using bio plex pro human cytokine 21 plex and 27 plex assay

1

Cytokine Quantification of SVF and PRP

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SVFs were collected from the upper phase of lipoaspirates following digestion. PRPs were harvested from blood samples. PRP and SVF samples were filtered using 0.45-μm filters (Corning) and then stored at –80 °C until use. After thawing, all the samples were analyzed in a single run in which cytokine quantification was assessed using the Bio-Plex Pro™ Human Cytokine 21-plex and 27-plex Assay (Bio-Rad). Raw data were analyzed by Bio-Plex Software (Bio-Rad).
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2

Comprehensive Cytokine Profiling of Tumor Microenvironment

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Tumor inflammation, cell proliferation, and immune response cytokines, produced by cancer and adipose cells, were quantified by using the Bio-Plex Pro™ Human Cytokine 21-plex and 27-plex Assay (Bio-Rad), or by customized Luminex Assay specific for VEGF, NGF, BNDF, NT-3, NT-4 (R&D). Raw data were analyzed by Bio-Plex Manager Software 6.2 (Bio-Rad).
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