Anti-p-ERK, anti-ERK, anti-p-JNK, anti-p-p38, anti-p-NF-κB p65, and anti-NF-κB p65 antibodies (Cell Signaling Technology, Danvers, MA, USA) and anti-JNK and anti-p38 antibodies (Santa Cruz Biotechnology, Santa Cruz, CA, USA) were used to detect specific proteins. Protein expression was determined using a Chemiluminescent HRP Substrate (Merck Millipore, Burlington, MA, USA) and a LAS-4000 luminescent-image reader (Fuji Photo Film, Tokyo, Japan). Image MultiGauge (Fuji Photo Film) was used to analyze immunoreactive signals.
Image multigauge
Manufactured by Fujifilm
Sourced in Japan
The Image MultiGauge is a versatile laboratory equipment product offered by Fujifilm. It provides accurate and reliable measurement capabilities for a range of applications. The core function of the Image MultiGauge is to facilitate precise quantitative analysis of images through its advanced imaging and software features.
Automatically generated - may contain errors
Lab products found in correlation
Las 4000 luminescent image reader, by Fujifilm (3 mentions)
Chemiluminescent hrp substrate, by Merck Group (2 mentions)
Anti nf κb p65 antibody, by Cell Signaling Technology (2 mentions)
Anti p nf κb p65, by Cell Signaling Technology (2 mentions)
Anti p p38, by Cell Signaling Technology (2 mentions)
Anti jnk and anti p38 antibodies, by Santa Cruz Biotechnology (2 mentions)
Anti erk, by Cell Signaling Technology (1 mentions)
Anti p jnk, by Cell Signaling Technology (1 mentions)
Anti p c jun n terminal kinase jnk, by Cell Signaling Technology (1 mentions)
Enhanced chemiluminescence western blotting kit, by Thermo Fisher Scientific (1 mentions)
Las 4000 luminescent image analyzer, by Fujifilm (1 mentions)
C ebpα, by Santa Cruz Biotechnology (1 mentions)
Gapdh, by Bioss Antibodies (1 mentions)
Immobilon western chemiluminescent hrp substrate, by Merck Group (1 mentions)
Fabp4, by Cell Signaling Technology (1 mentions)
4 protocols using image multigauge
1
Protein Expression Analysis with Antibodies
2
Quantifying MAPK and NF-κB Signaling
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For detection of MAPKs and NF-κB signaling pathways, anti-p- extracellular signal-regulated kinase (ERK), anti-ERK, anti-p-c-Jun N-terminal kinase (JNK), anti-p-p38, anti-p-NF-κB p65, and anti-NF-κB p65 antibodies (Cell Signaling Technology, Danvers, MA, USA) and anti-JNK and anti-p38 antibodies (Santa Cruz Biotechnology, Dallas, TX, USA) were used. Protein expression was determined using a Chemiluminescent HRP Substrate (Merck Millipore, Burlington, MA, USA) and a LAS-4000 luminescent-image reader (Fuji Photo Film, Tokyo, Japan). Image MultiGauge (Fuji Photo Film, Tokyo, Japan) was used to analyze immunoreactive signals.
3
Western Blot Analysis of Liver Proteins
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The proteins from liver and cell extracts were subjected to Western blotting analysis with antibodies against FAS and SREBP-1. Protein expression levels were determined by an enhanced-chemiluminescence Western blotting kit (Thermo Fisher Scientific Inc., Waltham, MA, USA) and LAS-4000 luminescent-image reader (Fuji Photo Film, Tokyo, Japan). The band intensities were analyzed using Fujifilm Image MultiGauge (Fuji Photo Film).
4
Protein Expression Analysis in Mouse Tissues
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Mouse-tissue protein extracts were subjected to immunoblot analysis with specific antibodies against phospho-AMPK, AMPK, PPARα, C/EBPα (Santa Cruz Biotechnology, Santa Cruz, CA, USA), FABP4 (Cell Signaling Technology, Danvers, MA, USA), and GAPDH (Bioss Antibodies, Boston, MA, USA) for the analysis of phosphorylated or total protein expression. GAPDH served as a loading control. Tissue proteins were fractionated by electrophoresis on 10% or 12% SDS-PAGE gels and transferred onto polyvinylidene fluoride membranes. The membranes were blotted with antibodies specific for each protein. Protein expression was determined using an Immobilon Western Chemiluminescent HRP Substrate (Merck Millipore, Burlington, MA, USA) and LAS-4000 Luminescent-Image Analyzer (Fuji Photo Film, Tokyo, Japan). The intensities of the detected bands were quantified using Fujifilm Image MultiGauge (Fuji Photo Film, Tokyo, Japan).
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