Ultrashield plus 600mhz nmr

All the solvents used during the experimental procedure were of analytical grade or distilled. Aluminum and glass plates, covered with silicon dioxide (Silica gel 60 F₂₅₄) were used for TLC and preparative TLC respectively and the obtained chromatograms were observed in a CAMAG TLC Visualizer at 254 and 366 nm. All the one- and two-dimensional NMR spectra of the purified compounds, as well as the calculations of the reaction yields, were acquired in a Bruker UltrashieldTM Plus 600MHz NMR.

1D and 2D NMR spectra were acquired in a Bruker UltrashieldTM Plus 600MHz NMR. MS analysis of the obtained compounds was performed with an LTQ ThermoScientific Orbitrap and an Agilent QQQ 6460. All solvents used during the experimental procedure were of analytical grade or redistilled. Additionally, the solvents used for Liquid Chromatography were HPLC grade and purified by filtration through ion- and carbon-exchange resins. The stationary phase for low pressure CC was silicon dioxide (Silica gel 40–63μm/Silica flash). Aluminum and glass plates, coated with silicon dioxide (Silica gel 60 F₂₅₄) were used for TLC and preparative TLC, respectively, and the obtained chromatograms were observed in a CAMAG TLC Visualizer at 254 and 366 nm. For the HPLC analysis, a THERMO Finnigan Spectra System was used, coupled with a PDA UV Detector. The column used was a Lichrosorb RP-18 with dimensions of 5 μm and 250×4 mm.