Cells were washed twice with PBS, directly solubilized in denaturing sample buffer and then subjected to SDS-PAGE. Proteins were electrotransferred to 0.2 µm Protran BA83 nitrocellulose sheets (Invitrogen) for immunodetection with primary antibodies NOX4 and NOX5 (cat. no. ab133303 and ab110400, respectively; Abcam®, Cambridge, MA). Immune complexes were detected with horseradish peroxidase coupled anti-rabbit IgG antibody (Amersham™, GE Healthcare Bio-Sciences, Pittsburgh, PA).
Protran ba83 nitrocellulose sheets
Manufactured by Thermo Fisher Scientific
Sourced in United States
Protran BA83 nitrocellulose sheets are a type of membrane used in scientific research and analysis. They are designed for the transfer and immobilization of biomolecules, such as proteins and nucleic acids, from electrophoresis gels onto a solid support for further processing and analysis.
Automatically generated - may contain errors
2 protocols using protran ba83 nitrocellulose sheets
1
Western Blot Analysis of NOX4 and NOX5
2
Western Blot Analysis of DNA Damage Response
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Cells were washed twice with PBS, solubilized in denaturing sample buffer and then subjected to SDS-PAGE. Proteins were electrotransferred to 0.2 µm Protran BA 83 nitrocellulose sheets (Invitrogen, Carlsbad, CA, USA) for immunodetection with the following primary antibodies: H2AX (1:2000; ab20669, Abcam, Cambridge, MA, USA); NRF2 (1:2000; MAB3925, R&D system, Minneapolis, MN, USA); NQO1 (1:3000; ab34173, Abcam, Cambridge, MA, USA); GCLC (1:2000; ab53179, Abcam, Cambridge, MA, USA). Immune complexes were detected with horseradish peroxidase coupled anti-rabbit or anti-mouse IgG antibodies (AmershamTM, GE Healthcare, Pittsburgh, PA, USA).
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