Esr900

Manufactured by Oxford Instruments

Sourced in United Kingdom

The ESR900 is a high-performance helium cryostat designed for various low-temperature research applications. It provides a stable and controlled environment for sample cooling down to 3.8 K. The cryostat features a top-loading sample chamber and can accommodate a wide range of sample holders and experimental setups.

Automatically generated - may contain errors

Lab products found in correlation

Elexsys e500, by Bruker (5 mentions) Elexsys e500 spectrometer, by Bruker (5 mentions) Elexsys e580 spectrometer, by Bruker (4 mentions) Itc503, by Oxford Instruments (4 mentions) Esp 300 spectrometer, by Bruker (4 mentions) Cf935, by Oxford Instruments (3 mentions) E500 x band epr spectrometer, by Bruker (3 mentions) Emxplus spectrometer, by Bruker (2 mentions) Er 4116dm, by Bruker (2 mentions) Elexsys e500 epr spectrometer, by Oxford Instruments (2 mentions) Cf935 cryostat, by Oxford Instruments (2 mentions) En 4118x md4, by Bruker (2 mentions) Grams ai spectroscopy software, by Thermo Fisher Scientific (2 mentions) 707 sq 250m epr tubes, by Wilmad (2 mentions) Esp 300, by Oxford Instruments (1 mentions) Pl450b, by Thorlabs (1 mentions) Sodium dithionite, by Merck Group (1 mentions) Uv 1800, by Shimadzu (1 mentions) Elexsys e500 spectrometer, by Oxford Instruments (1 mentions) Emx plus 6 1 spectrometer, by Oxford Instruments (1 mentions)

Spelling variants of this product

ESR900 cryostat (28 citations) ESR900 helium flow cryostat (26 citations) ESR900 liquid helium cryostat (9 citations) ESR900 helium cryostat (4 citations) ESR900 helium (3 citations)

41 protocols using esr900

Cryogenic X-band EPR Spectroscopy

Check if the same lab product or an alternative is used in the 5 most similar protocols

X-band EPR spectra were obtained on a Bruker ESP 300 spectrometer
equipped with an Oxford Instruments ESR 900 continuous-liquid-helium-flow
system. Spectra were recorded at the following conditions (if not
specified): temperature, 3.8 K; microwave frequency, ∼9.36
GHz; microwave power, 2 mW; modulation amplitude, 9 G; time constant,
160 ms; field sweep speed, 28 G/s. Intra-EPR-cavity irradiation of
a sample placed inside the cryostat was performed with a Thorlabs
Inc. (Newton, NJ) PL450B, 450 nm, 80 mW Osram laser diode mounted
on the cavity access port. Cryoannealing protocol involved multiple
steps in which the sample frozen in liquid nitrogen was rapidly warmed
by immersion in a pentane bath held at the annealing temperature for
a fixed time and then cooled back to 77 K by immersion in liquid nitrogen.
Overlapping signals 1a and 1b in the obtained spectra were decomposed
as previously described.^{12 (link)} Data points
of the E₂/1b state were obtained as the intensity of the
1b signal g₂ feature measured as the peak-to-peak
height and normalized to its maximum before irradiation. Data points
for changes in the 1c signal (denoted in the text as 1c*) were obtained
as the intensity of the resolved g₁ feature,
normalized for kinetic plots as corresponding to the observed photoinduced
1b → 1c* conversion and shown after preirradiation background
subtraction.

Lukoyanov D.A., Khadka N., Yang Z.Y., Dean D.R., Seefeldt L.C, & Hoffman B.M. (2018). Hydride Conformers of the Nitrogenase FeMo-cofactor Two-Electron Reduced State E2(2H), Assigned Using Cryogenic Intra Electron Paramagnetic Resonance Cavity Photolysis. Inorganic Chemistry, 57(12), 6847-6852.

+ Open protocol

+ Expand

CW-EPR Spectroscopy of CS2 Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols

Continuous-wave (CW) EPR experiments were performed on a Bruker ElexSys E580 spectrometer at the X band (ω = 9.36 GHz) with the samples dissolved in CS₂. The low-temperature environment was achieved by using an Oxford Instruments ESR900 and CF935 liquid helium cryostat.

Meng Q., Abella L., Yao Y.R., Sergentu D.C., Yang W., Liu X., Zhuang J., Echegoyen L., Autschbach J, & Chen N. (2022). A charged diatomic triple-bonded U≡N species trapped in C82 fullerene cages. Nature Communications, 13, 7192.

+ Open protocol

+ Expand

Spectroscopic Characterization of AtDGAT3

Check if the same lab product or an alternative is used in the 5 most similar protocols

UV-Visible absorption spectra were recorded in quartz cuvettes using a UV-1800 Shimadzu spectrophotometer.
For electron paramagnetic resonance (EPR) analysis, two solutions were analyzed (i) without the addition of sodium dithionite, (ii) with 1 mM sodium dithionite incubated 10 min with Δ46AtDGAT3 sample, using a freshly prepared stock of 100 mM sodium dithionite (Sigma-Aldrich) kept in a MBraun glovebox (O₂ < 0.5 ppm). Each solution was introduced into EPR quartz tubes. A standard Cu-EDTA solution was used (200 µM) in 25 mM Tris-HCl pH 8, 200 mM NaCl, in order to quantify AtDGAT3 signal. Continuous wave EPR experiments were performed on a X-Band ELEXSYS E500 spectrometer (Bruker BioSpin S.A.S., Wissembourg, France) operating at 9.39 GHZ and equipped with ST cavity cooled by an helium flow cryostat ESR 900 (Oxford Instruments, Austin, USA). The continuous wave EPR spectra of frozen solution were recorded at 50 K under non-saturating conditions and using the following parameters: a microwave power of 10 mW, a modulation amplitude of 5 G, a receiver gain of 40 dB and an accumulation of 10 scans.

Aymé L., Arragain S., Canonge M., Baud S., Touati N., Bimai O., Jagic F., Louis-Mondésir C., Briozzo P., Fontecave M, & Chardot T. (2018). Arabidopsis thaliana DGAT3 is a [2Fe-2S] protein involved in TAG biosynthesis. Scientific Reports, 8, 17254.

+ Open protocol

+ Expand

Continuous-wave EPR Characterization Methodology

Check if the same lab product or an alternative is used in the 5 most similar protocols

Continuous-wave (CW) EPR experiments were performed on a Bruker ElexSys E580 spectrometer at the X-band (ω = 9.36 GHz) with the samples dissolved in CS₂. The low-temperature environment was achieved by using an Oxford Instruments ESR900 and CF935 liquid helium cryostat. The EPR spectra were all simulated using the “EasySpin” toolbox based on MATLAB^{48 (link)}. DC magnetic properties were determined using a Quantum Design MPMS3 VSM magnetometer. The sample was prepared by drop-casting from CS₂ solution onto a slice of Al foil (3.224 mg), which is paramagnetic to minimize the background of the sample holder. Fast evaporation of the carbon disulfide afforded a black powder. After that, the Al foil was folded into a small cube and stuck on the inner wall of a plastic straw with very small amount of N grease (less than 1 mg).

Yan Y., Abella L., Sun R., Fang Y.H., Roselló Y., Shen Y., Jin M., Rodríguez-Fortea A., de Graaf C., Meng Q., Yao Y.R., Echegoyen L., Wang B.W., Gao S., Poblet J.M, & Chen N. (2023). Actinide-lanthanide single electron metal-metal bond formed in mixed-valence di-metallofullerenes. Nature Communications, 14, 6637.

+ Open protocol

+ Expand

X-band EPR Spectroscopy of T2 Sample

Check if the same lab product or an alternative is used in the 5 most similar protocols

X-band continuous-wave EPR spectra were collected from a T2 sample (390 μM in 50 mM MOPS, pH 7, and 50 mM NaCl) with a Bruker (Billerica, MA) EleXsys E500 spectrometer equipped with a cylindrical TE110-mode resonator (ER4122SHQE), an ESR-900 liquid helium cryostat, and an ITC-5 temperature controller (Oxford Instruments ITC503). The spectra were recorded at 40 K at 9.4 GHz using 5.0 G modulation amplitude. Spectral simulations were performed using EasySpin 5.2.35 within MATLAB 2015a software suite (94 (link)).

Martinez-D’Alto A., Yan X., Detomasi T.C., Sayler R.I., Thomas W.C., Talbot N.J, & Marletta M.A. (2023). Characterization of a unique polysaccharide monooxygenase from the plant pathogen Magnaporthe oryzae. Proceedings of the National Academy of Sciences of the United States of America, 120(8), e2215426120.

+ Open protocol

+ Expand

CW-EPR Characterization of Catalyst Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols

X-band (9.64 GHz) CW-EPR spectra were acquired on a Bruker EMX/Plus 6/1 spectrometer equipped with a liquid helium quartz cryostat (Oxford Instruments ESR900) using a temperature and gas flow controller (Oxford Instruments ITC503). The preparation procedures of CW-EPR samples are as follows: 3 mg of catalyst powder was added into 200 μL of a buffer solution (Buffer solution was prepared using either 0.2 M citric acid (Wako, Japan) for pH 4, 5, and 5.5, or 0.2 M phosphate [Wako] for pH 6 and 7, respectively) and the mixture was sonicated for 5 min to generate a homogeneous suspension. After purging the suspension with N₂ to remove the dissolved oxygen, 8.3 μL of a 0.5 M dithionite (Sigma-Aldrich) solution was added under N₂ bubbling to reduce the catalyst. After 5 min, 20 μL of glycerol (Sigma-Aldrich) was added and 200 μL of the suspension was transferred to a CW-EPR tube and frozen in liquid nitrogen immediately. The CW-EPR spectra were collected under the following experimental conditions: microwave frequency, 9.64 GHz; microwave power, 1 mW; modulation frequency, 100 kHz; modulation amplitude, 1.0 mT; time constant, 40.96 ms; conversion time, 48.00 ms; sweep time, 96 s; four scans; temperature 30 K.

He D., Ooka H., Kim Y., Li Y., Jin F., Kim S.H, & Nakamura R. (2020). Atomic-scale evidence for highly selective electrocatalytic N−N coupling on metallic MoS2. Proceedings of the National Academy of Sciences of the United States of America, 117(50), 31631-31638.

+ Open protocol

+ Expand

Anaerobic EPR characterization of bovine complex I

Check if the same lab product or an alternative is used in the 5 most similar protocols

EPR samples of ~11 mg/ml bovine complex I were prepared anaerobically. The samples were incubated for 15 min at 4°C with 100 mM NaCl, 100 mM metformin, 2.5 mM phenformin, or 0.35 mM proguanil, then 5 mM NADH was added and the samples frozen immediately. Spectra were recorded with 1 mW microwave power, microwave frequency 9.36–9.38 GHz, modulation frequency 100 kHz, modulation amplitude 1 mT, time constant 81.92 ms and conversion time 20.48 ms at 12 K, using a Bruker EMX X-band spectrometer with an ER 4119HS high-sensitivity cavity, maintained at low temperature by an Oxford Instruments ESR900 continuous-flow liquid helium cryostat.

Bridges H.R., Jones A.J., Pollak M.N, & Hirst J. (2014). Effects of metformin and other biguanides on oxidative phosphorylation in mitochondria. Biochemical Journal, 462(Pt 3), 475-487.

+ Open protocol

+ Expand

Spectroscopic Characterization of Flavodiiron Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols

UV-Visible spectra were obtained in a PerkinElmer Lambda 35 spectrophotometer. Electron Paramagnetic Resonance (EPR) spectroscopy characterization of both FDPs was performed using a Bruker EMX spectrometer equipped with an Oxford Instruments ESR-900 continuous flow helium cryostat, and a high sensitivity perpendicular mode rectangular cavity. Protein samples were prepared aerobically to final concentrations of 300 µM (FDP_F) and 200 µM (FDP_F_Cter). A partially reduced sample of FDP_F was also prepared anaerobically by incubation with 150 µM menadiol. EPR spectra were simulated using the program SpinCount⁵².

Folgosa F., Martins M.C, & Teixeira M. (2018). The multidomain flavodiiron protein from Clostridium difficile 630 is an NADH:oxygen oxidoreductase. Scientific Reports, 8, 10164.

+ Open protocol

+ Expand

Pulsed X-Band EPR Spectroscopy

Check if the same lab product or an alternative is used in the 5 most similar protocols

CW X-Band EPR spectra were recorded on a Bruker Elexsys E500 spectrometer equipped with a SHQ cavity (ν = 9.39 GHz). Low temperature measurements were obtained using an Oxford Instruments ESR900 continuous flow helium cryostat. Pulsed EPR measurements were carried out with a Bruker Elexsys E580 at X-band (ν ≅ 9.70 GHz) equipped with a flexline dielectric ring ENDOR resonator (Bruker EN 4118X-MD4). Temperatures between 4.5 and 100 K were obtained with an Oxford Instruments CF935 continuous flow helium cryostat. Echo detected field swept EPR spectra were recorded by using the Hahn Echo pulse sequence (π/2 – τ – π – τ – echo) with a fixed interpulse delay time τ = 200 ns, t_π/2 = 16 ns and t_π = 32 ns. Both phase memory times were measured by using the Hahn Echo sequence upon increasing the interpulse delay τ starting from τ = 98 ns. Spin-lattice relaxation times were measured using the standard inversion recovery sequence (π – t_d – π/2 – τ – π – τ – echo), with π/2 = 16 ns. The uncertainty in T₁ estimated from replicate measurements was 5–10% depending upon the signal-to-noise ratio at a given temperature-field combination.

Atzori M., Chiesa A., Morra E., Chiesa M., Sorace L., Carretta S, & Sessoli R. (2018). A two-qubit molecular architecture for electron-mediated nuclear quantum simulation †Electronic supplementary information (ESI) available: Additional figures, tables, equations and computational details as mentioned in the text. See DOI: 10.1039/c8sc01695j. Chemical Science, 9(29), 6183-6192.

+ Open protocol

+ Expand

Raman and EPR Spectroscopy of Molecular Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols

RR spectra were obtained using a McPherson 2061/207 spectrograph (0.67 m with variable gratings) equipped with a Princeton Instruments liquid N₂-cooled CCD detector (LN-1100PB). The 407-nm line of a krypton laser (Innova 302, Coherent) was used as the Raman excitation source. A long-pass filter (RazorEdge, Semrock) was used to attenuate Rayleigh scattering. Spectra at room temperature were collected in a 90° scattering geometry on samples mounted on a reciprocating translation stage. Frequencies were calibrated relative to indene and CCl₄ and are accurate to ±1 cm⁻¹. CCl₄ was also used to check the polarization conditions. Low temperature spectra were obtained in a backscattering geometry on samples maintained at ~ 110 K in a liquid nitrogen cold finger. Frequencies were calibrated relative to aspirin and are accurate to ±1 cm⁻¹. The integrity of the RR samples, before and after laser illumination, was confirmed by direct monitoring of their UV-Vis spectra in the Raman capillaries. EPR spectra were recorded on a Bruker E500 X-band EPR spectrometer equipped with a superX microwave bridge, a dual mode cavity and a helium-flow cryostat (ESR 900, Oxford Instruments, Inc.).

Kumar R., Qi Y., Matsumura H., Lovell S., Yao H., Battaile K.P., Im W., Moënne-Loccoz P, & Rivera M. (2016). Replacing Arginine 33 for Alanine in the Hemophore HasA from Pseudomonas aeruginosa Causes Closure of the H32 Loop in the Apo-Protein. Biochemistry, 55(18), 2622-2631.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!