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Ab236712

Manufactured by Abcam
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Sourced in United States, United Kingdom

Ab236712 is a laboratory product manufactured by Abcam. It serves as a tool for researchers in various scientific applications.

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Lab products found in correlation

Ab255730, by Abcam (12 mentions) Ab234570, by Abcam (9 mentions) Ab214566, by Abcam (7 mentions) Softmax pro software, by Molecular Devices (3 mentions) Versamax, by Molecular Devices (2 mentions) Sekr 0071, by Solarbio (2 mentions) Ab65354, by Abcam (1 mentions) Ab263882, by Abcam (1 mentions) Ab113852, by Abcam (1 mentions) Silymarin, by Merck Group (1 mentions) Ab119558, by Abcam (1 mentions) Ab186027, by Abcam (1 mentions) Ab213899, by Abcam (1 mentions) Ab207223, by Abcam (1 mentions) Ab65340, by Abcam (1 mentions) Ab83362, by Abcam (1 mentions) Ck mb kit, by Nanjing Jiancheng (1 mentions) Elisa kit, by Abcam (1 mentions) Ab234579, by Abcam (1 mentions) Ab263883, by Abcam (1 mentions)

19 protocols using ab236712

1

Hippocampal Cytokine Profiling

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The hippocampus undergoing different treatments were homogenized using PBS and centrifuged at 8000 g at 4°C for 15 min to harvest the supernatant. Afterwards, the levels of M1 pro-inflammatory cytokines interleukin (IL)-1β (ab255730) and tumor necrosis factor-α (TNF-α) (ab236712), and M2 anti-inflammatory cytokines IL-10 (ab214566) and IL-4 (ab100771, all from Abcam) were analyzed using the specific ELISA kit and calculated according to the standard curve [21 (link)].
Huang X., Yang Q., Xie L, & Lei S. (2022). Histone methyltransferase enhancer of zeste 2 polycomb repressive complex 2 subunit exacerbates inflammation in depression rats by modulating microglia polarization. Bioengineered, 13(3), 5509-5524.
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2

Microglia Cytokine Quantification

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Different treatments were used during the 24-hour cultivation of primary microglia cells. The culture supernatant was then taken out and spun at 3000 rpm for 20 min. Finally, using the appropriate ELISA kit per the manufacturer’s instructions, the cellular abundances of TNF-α (ab236712, Abcam), IL-1β (ab255730, Abcam), and IL-6 (ab234570, Abcam) were found in the medium.
Tang L., Xu Y., Wang L, & Pan J. (2023). Adipose-derived stem cell exosomes ameliorate traumatic brain injury through the NLRP3 signaling pathway. Neuroreport, 34(13), 677-684.
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3

Evaluating Hepatoprotective Potential

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Carbon tetrachloride (CCl4) (#PHR1063), silymarin (#S0292), and other chemicals and reagents were purchased from Sigma Aldrich Chemicals Co., St. Louis, Missouri, United States. Kits for the pro-inflammatory (TGF-β #ab119558, TNF-α #ab236712, IL-6 #ab234570, and adiponectin #ab239421) and anti-inflammatory cytokine markers (SOD #ab65354, CAT #ab118184, GSR #ab65322, and TBARS/MDA #ab238537), liver function test (LFT) (AST #ab263882, ALT #234579, ALP #ab83369, and Bil #ab235627) enzymes to detect free radical scavenging activity, mitochondrial membrane potential (MMP) (MMP #ab113852), and ROS (ab #ab186027) detection were purchased from Abcam, Cambridge, United Kingdom.
Alnuqaydan A.M., Almutary A.G., A. Alsahli M., Alnasser S, & Rah B. (2022). Tamarix articulata Induced Prevention of Hepatotoxicity Effects of In Vivo Carbon Tetrachloride by Modulating Pro-Inflammatory Serum and Antioxidant Enzymes to Reverse the Liver Fibrosis. Antioxidants, 11(9), 1824.
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4

Wistar Rat Neurochemical Profiling

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The experimental protocol was approved by the Animal Ethical Committee of No.4
People’s Hospital of Hengshui, Ethic No. HB2020-11(05). All experiments were
conducted as per the ethical guidelines of the Animal Ethical Committee.
Male Wistar albino rats were employed for the current study and were kept in the
animal house of People’s Hospital of Hengshui. The animals were provided with
standard feed and water. The animals were exposed to 12 h of light and 12 h of
the dark at 25 ± 2 °C and 55–60% relative humidity. The ELISA kits for the
quantification of BDNF (ab213899), TNF-α (ab236712) and Nrf2 (ab207223) were
procured from Abcam, USA. The ELISA kit for CSE (abx155408) was procured from
Abbexa LLC, Houston, USA; while the fluorometric assay kit for CBS (K-998) was
obtained from BioVision, Inc, California USA. Octreotide and ANA-12 were
procured from Sigma-Aldrich, USA.
Jiang R, & Wei H. (2021). Beneficial effects of octreotide in alcohol-induced neuropathic pain. Role of H2S, BDNF, TNF-α and Nrf2. Acta Cirúrgica Brasileira, 36(4), e360408.
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5

Cytokine Levels in Plasma Analysis

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The levels of the cytokines IL-6 (R6000B, R&D Systems, MN, USA), IL-10 (ab214566, Abcam, MA, USA), and TNF-α (ab236712, Abcam, Cambridge, MA, USA) in plasma were measured using ELISA kits according to the manufacturer’s instructions. The optical density at 450 nm was measured using a microplate reader (VersaMax with SoftMax Pro software, Molecular Devices, San Jose, CA, USA).
Park Y.J., Lee M.J., Bae J., Lee J.H., Lee H.A., Mun S., Kim Y.S., Yune C.J., Chung T.N, & Kim K. (2022). Effects of Glucocorticoid Therapy on Sepsis Depend Both on the Dose of Steroids and on the Severity and Phase of the Animal Sepsis Model. Life, 12(3), 421.
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6

Cardiac and Inflammatory Biomarkers in Rats

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On week 8, the body and hearts of the rats were weighed. Then, the rats were sedated through intraperitoneal injection of pentobarbital (1%). The blood specimens were harvested from the cardiac apex of rats, put into 2.0 mL heparinized Eppendorf (EP) tubes, mixed completely, and centrifuged at 8000 r/min and 4 °C for 6 min. The supernatant was stored at −80 °C. The creatine phosphokinase-MB (CK-MB) kit (H197-1-1, NanJing JianCheng Bioengineering Institute, Nanjing, China) was utilized to detect CK-MB levels. Sarcosine oxidase and urease methods were employed to measure blood creatinine (ab65340, Abcam, Cambridge, UK) and blood urea nitrogen (BUN; ab83362, Abcam), respectively. Inflammatory cytokines, interleukin-6 [(IL-6), ab234570], tumor necrosis factor-alpha [(TNF-α), ab236712], and interleukin-1 beta [(IL-1β), ab255730] were tested by corresponding enzyme-linked immunosorbent assay (ELISA) kits (Abcam). The above relevant biochemical indexes were measured strictly as per the specific operation steps and precautions of the corresponding kits, and the absorbance was calculated on a microplate reader. The sample concentration was calculated according to the formula or the standard curve regression equation corresponding to the biochemical indexes.
Cai X., Hong L., Liu Y., Huang X., Lai H, & Shao L. (2022). Salmonella pathogenicity island 1 knockdown confers protection against myocardial fibrosis and inflammation in uremic cardiomyopathy via down-regulation of S100 Calcium Binding Protein A8/A9 transcription. Renal Failure, 44(1), 1819-1832.
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7

Cytokine Profiling in Plasma

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The levels of the cytokines interleukin (IL)-6 (R6000B, R&D Systems, Inc., Minneapolis, MN, USA), IL-10 (ab214566, Abcam, MA, USA), and TNF-α (ab236712, Abcam, MA, USA) in plasma were measured using ELISA kits, in accordance with the manufacturer’s instructions. The optical density at 450 nm was measured using a VersaMax microplate reader (SoftMax Pro software, Molecular Devices, CA, USA).
Lee M.J., Bae J., Lee J.H., Park Y.J., Lee H.A., Mun S., Kim Y.S., Yune C.J., Chung T.N, & Kim K. (2022). Serial Change of Endotoxin Tolerance in a Polymicrobial Sepsis Model. International Journal of Molecular Sciences, 23(12), 6581.
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8

Measurement of Serum Cytokines

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Blood was collected and stored at 4 °C overnight. The supernatant was collected and stored at -80 °C until use. Serum levels of TNF-α and IL-1β were measured by using a commercially available ELISA kit (TNF-α; ab236712, IL-1β; ab255730, Abcam, Cambridge, United Kingdom), according to the manufacturer’s protocol. Serum cytokines were evaluated as secondary experimental outcomes.
Yamanashi T., Iwata M., Shibushita M., Tsunetomi K., Nagata M., Kajitani N., Miura A., Matsuo R., Nishiguchi T., Kato T.A., Setoyama D., Shirayama Y., Watanabe K., Shinozaki G, & Kaneko K. (2020). Beta-hydroxybutyrate, an endogenous NLRP3 inflammasome inhibitor, attenuates anxiety-related behavior in a rodent post-traumatic stress disorder model. Scientific Reports, 10, 21629.
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9

Cytokine Levels in Spleen and Plasma

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The levels of the cytokines IL-6 (R6000B, R&D Systems, MN, USA), IL-10 (ab214566, Abcam, MA, USA), and TNF-α (ab236712, Abcam, MA, USA) in spleen homogenates and plasma were measured using ELISA kits according to the manufacturer’s instructions. To assess cytokine levels in the spleen, frozen tissue was homogenized in RIPA buffer with protease inhibitor cocktail. The optical density at 450 nm was detected by a microplate reader (VersaMax with SoftMax Pro software, Molecular Devices, CA, USA).
Hwang J.W., Lee M.J., Chung T.N., Lee H.A., Lee J.H., Choi S.Y., Park Y.J., Kim C.H., Jin I., Kim S.H., Kwak H.B., Heo J.W., Na K., Choi S., Choi Y.S, & Kim K. (2021). The immune modulatory effects of mitochondrial transplantation on cecal slurry model in rat. Critical Care, 25, 20.
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10

Serum IL-1β and TNF-α Quantification

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The expression levels of interleukin (IL)-1β (product code ab255730; Abcam) and TNF-α (product code ab236712; Abcam) were detected in rat serum using respective ELISA kits.
Zhang Y., Li Y., Wang Q., Zheng D., Feng X., Zhao W., Cai L., Zhang Q., Xu H, & Fu H. (2021). Attenuation of hepatic ischemia-reperfusion injury by adipose stem cell-derived exosome treatment via ERK1/2 and GSK-3β signaling pathways. International Journal of Molecular Medicine, 49(2), 13.
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