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Cd14 and cd16 microbeads

Manufactured by Miltenyi Biotec
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CD14 and CD16 microbeads are magnetic beads coated with antibodies specific to the CD14 and CD16 cell surface markers, respectively. They are designed for the isolation and enrichment of CD14+ and CD16+ cells from complex cell samples using magnetic separation techniques.

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Lab products found in correlation

Fitc anti human cd14 antibody, by BioLegend (1 mentions) Automacs, by Miltenyi Biotec (1 mentions)

Spelling variants of this product

CD14 MicroBeads (570 citations) CD16 microbeads (24 citations)

2 protocols using cd14 and cd16 microbeads

1

Isolation of Human Lymphocytes

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Human lymphocytes were isolated from the buffy coat by collecting the flow through from CD14 and CD16 microbeads (Miltenyi Biotec).
Cui C., Chakraborty K., Tang X.A., Zhou G., Schoenfelt K.Q., Becker K.M., Hoffman A., Chang Y.F., Blank A., Reardon C.A., Kenny H.A., Vaisar T., Lengyel E., Greene G, & Becker L. (2021). Neutrophil elastase selectively kills cancer cells and attenuates tumorigenesis. Cell, 184(12), 3163-3177.e21.
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2

Isolation of Pure CD14++ Monocytes

Cited 1 time
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Monocyte separation was conducted as described previously [19 (link)]. Peripheral blood mononuclear cells were isolated from a 30 ml Lithium-heparin-anti-coagulated blood sample by Ficoll-based density gradient centrifugation. Magnetic cell sorting (autoMACS, Miltenyi Biotec, Bergisch Gladbach, Germany) using CD14 and CD16 MicroBeads (Miltenyi Biotec, Bergisch Gladbach, Germany) was performed to deplete CD16++ monocytes and to isolate CD14++ CD16—monocytes, followed by flow cytometry after incubation with FITC anti-human CD14 Antibody (BioLegend, San Diego, USA) to ensure purity of the isolated cells.
Siegler B.H., Uhle F., Lichtenstern C., Arens C., Bartkuhn M., Weigand M.A, & Weiterer S. (2018). Impact of human sepsis on CCCTC-binding factor associated monocyte transcriptional response of Major Histocompatibility Complex II components. PLoS ONE, 13(9), e0204168.
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