1100 hplc
The 1100 HPLC is a high-performance liquid chromatography (HPLC) system manufactured by Agilent Technologies. It is designed for the separation, identification, and quantification of complex mixtures of chemical compounds. The 1100 HPLC system includes a pump, an autosampler, a column compartment, and a detector, all integrated into a single unit. The system is capable of performing a wide range of analytical applications, including pharmaceutical, environmental, and chemical analysis.
Lab products found in correlation
111 protocols using 1100 hplc
Saponification and HPLC Analysis of Fatty Acids
Quantifying Cysteine Oxidation Kinetics
Purification and Characterization of Proteins
Metabolite Analysis of Fermentation Samples
Peptide Fractionation via ERLIC-LC-MS/MS
prior to LC–MS/MS via electrostatic repulsion–hydrophilic
interaction chromatography (ERLIC).22 (link) Desalted
samples were redissolved in 50 μL of 85% acetonitrile/0.1% formic
acid and loaded on a polyWAX LP column (150 × 1.0 mm; 5 μm
300 Å; PolyLC) attached to an Agilent 1100 HPLC at a 0.05 mL/min
flow rate. The samples were separated over an 80 min gradient as follows
(solvent A: 80% acetonitrile, 0.1% formic acid; solvent B: 30% acetonitrile,
0.1% formic acid). Isocratic flow was maintained at 100% A at a flow
rate of 0.3 mL/min for 5 min, followed by a 17 min linear gradient
to 8% B and a 25 min linear gradient to 45% B. Finally, a 10 min gradient
to 100% B was followed by a 5 min hold at 100% B before a 10 min linear
gradient back to 100% A, followed by an 8 min hold at 100% A. Fractions
were collected every several minutes, resulting in 15–17 samples
for further LC–MS/MS analysis. Each fraction was vacuum-dried
using a Savant SPD1010 SpeedVac concentrator (Thermo Scientific).
Polysaccharide Molecular Weight Analysis
Separating Bioactive Peptides by RP-HPLC
Quantifying Drug Loading in PP-CD Nanoparticles
HPLC Analysis of Carbonyl Compounds in Wine
The analyses were carried out on an Agilent 1100 HPLC equipped with a LiChrosper 250 × 4 mm (5 μm particle size) column. The flow rate was 0.4 mL/min, the injection volume was 25 μL and the signal was acquired at 365 nm using a DAD detector. Phase A was 1% acetonitrile in phosphate buffer 25 mM, pH 2.20, phase B was acetonitrile. The following gradient was established: at 0 min 75% A and 25% B, after 10 min 50% A and 50% B, after 30 min 75% A and 25% B, after 45 min 25% A and 75% B. A sample chromatogram is provided in Figure
HPLC Analysis of Oligonucleotide Digestion
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