Sesame oil

Manufactured by Merck Group

Sourced in United States, India, Germany, Japan, United Kingdom

Sesame oil is a versatile laboratory product that serves as a carrier or base oil in various applications. It is a natural, vegetable-derived oil that is commonly used in scientific and industrial settings. Sesame oil has a range of physical and chemical properties that make it suitable for use in lab equipment and procedures. However, a detailed description of its specific functions and intended uses would require further research to maintain an unbiased and factual approach.

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Lab products found in correlation

Tamoxifen, by Merck Group (13 mentions) Estradiol benzoate, by Merck Group (6 mentions) Oleic acid, by Merck Group (6 mentions) Olive oil, by Merck Group (5 mentions) Dimethyl sulfoxide (dmso), by Merck Group (5 mentions) S3547, by Merck Group (4 mentions) β estradiol, by Merck Group (4 mentions) Testosterone, by Merck Group (4 mentions) Castor oil, by Merck Group (4 mentions) β estradiol 17 valerate, by Merck Group (4 mentions) Tween 80, by Merck Group (4 mentions) 17β estradiol, by Merck Group (4 mentions) Progesterone, by Merck Group (4 mentions) 1 octanesulfonic acid, by Merck Group (3 mentions) Ethylene diamine tetraacetic acid (edta), by Merck Group (3 mentions) Benzyl benzoate, by Merck Group (3 mentions) Peanut oil, by Merck Group (3 mentions) Estradiol valerate, by Merck Group (3 mentions) C57bl 6 mice, by Charles River Laboratories (3 mentions) Estradiol, by Merck Group (3 mentions)

164 protocols using sesame oil

Uterine Steroid Hormone Regulation

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Institute of Cancer Research (ICR) mice were purchased from Raon Bio. The mice were housed in a semi-specific pathogen-free facility and maintained in accordance with the Institutional Animal Care and Use Committee (IACUC) guidelines until use. The mouse experiments were approved by the IACUC at Konkuk University (KU22030). To obtain uterine tissues from pregnant mice, 7-week-old ICR mice were injected with 2.5 IU of pregnant mare serum gonadotropin (PMSG) (Daesung Microbiological Labs Ltd.) and 2.5 IU of human chorionic gonadotropin (hCG) (Sigma-Aldrich) at 48-hour intervals. After the hCG injection, the female mice were caged with stud male mice for mating. Female mice with vaginal plugs the following morning were designated day 1 of pregnancy. Uteri were collected on days 1, 4, and 8 of pregnancy. The implantation sites from day 8 pregnant mice were separated into embryo-containing decidua and myometrium [16] (link).
To examine the effects of steroid hormones on Atg9a and Atg9b, mice underwent ovariectomy (OVX) and rested for 10 to 12 days. The mice received a single injection of vehicle (sesame oil 0.1 mL; Sigma-Aldrich), progesterone (P) (1 mg/0.1 mL sesame oil; Sigma-Aldrich), or 17β-estradiol (E) (100 ng/0.1 mL sesame oil; Sigma-Aldrich) subcutaneously. The uteri were collected after 24 hours and subjected to RNA extraction or lysate preparation.

Lee M., Son S., Lim H.J, & Song H. (2024). The differential expression patterns of Atg9a and Atg9b in cells of the reproductive organs. Clinical and experimental reproductive medicine.

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Estradiol and Leptin Effects on Molecular Pathways

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For the molecular studies (i.e. cFOS immunohistochemistry, cell fractioning, Co-IP, and High-throughput ChIPmentation), food was removed and after one hour mice were subjected to subcutaneous injections of vehicle (150 μl of sesame oil, Sigma Aldrich) or estradiol (estradiol benzoate; 1 μg dissolved in 150 μL of sesame oil; Sigma Aldrich). Three hours later mice were subjected to intraperitoneal injections of vehicle (equivalent volume of 20 mM Tris-HCl diluted in ice-cold PBS, pH 7.4) or leptin (3 mg/kg BW, stock leptin solution diluted in ice-cold PBS, pH 7.4). One hour (cell fractioning, Co-IP and High-throughput ChIPmentation) or 90 minutes (cFOS immunohistochemistry) later mice were sacrificed and tissues were collected. When cycled female mice were used for the study, the same experimental paradigm was followed but estradiol injections were skipped.

González-García I., García-Clavé E., Cebrian-Serrano A., Le Thuc O., Contreras R.E., Xu Y., Gruber T., Schriever S.C., Legutko B., Lintelmann J., Adamski J., Wurst W., Müller T.D., Woods S.C., Pfluger P.T., Tschöp M.H., Fisette A, & García-Cáceres C. (2023). Estradiol regulates leptin sensitivity to control feeding via hypothalamic Cited1. Cell Metabolism, 35(3), 438-455.e7.

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Prepubertal Mouse PCOS Model

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Forty five female prepubertal (23-24 days old) mice of the BALB/c were used in the this study. The mice were holded in polycarbonate boxes and maintained in 12-hour light/12-hour dark cycle, and 50%-70% humidity, 23±1 °C temperature, with free access to water and food. The animals were randomly divided into 3 groups each consisting of 15 mice: control group (no injections were performed to mice); vehicle group [mice were injected subcutaneously (SC) with 0.1 mL of sesame oil (Sigma-Aldrich, İstanbul, Turkey) daily for 20 consecutive days] and PCOS group [DHEA (dissolved in 0.1 mL sesame oil, 6 mg/100 g body weight; Merck Millipore, İstanbul, Turkey) was injected to each mice for 20 consecutive days SC]. The phases of the estrous cycle for each mice were determined by daily histological examination of vaginal smears. The ethical approval was obtained from Mersin University School of Medicine and its number is 2012-11.

Karagül M.İ., Aktaş S., Coşkun Yılmaz B., Yılmaz M, & Orekici Temel G. (2018). GDF9 and BMP15 Expressions and Fine Structure Changes During Folliculogenesis in Polycystic Ovary Syndrome. Balkan Medical Journal, 35(1), 43-54.

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Diazepam Effects on Mice Behavior

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A separate cohort of mice that had not previously been exposed to the OF apparatus were acclimated to handling for 3 days prior to treatment. Mice were treated with 1.5 mg/kg diazepam (7-chloro-1-methyl-5-phenyl-3H-1,4-benzodiazepin-2(1H)-one) (Sigma), diluted in sesame oil (Sigma) via subcutaneous injection, or sesame oil only. Mice were placed into an individual standard cage and transported to the behavioral room containing the OF. Mice were placed in the OF and allowed to explore for 45 min, and analysis was split into 15-min bins.

Mahoney H.L., Bloom C.A., Justin H.S., Capraro B.M., Morris C., Gonzalez D., Sandefur E., Faulkner J., Reiss S., Valladares A., Ocampo A., Carter B., Lussier A.L., Dinh L.P., Weeber E., Gamsby J, & Gulick D. (2024). DISC1 and reelin interact to alter cognition, inhibition, and neurogenesis in a novel mouse model of schizophrenia. Frontiers in Cellular Neuroscience, 17, 1321632.

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Behavioral Estrus Induction in Mice

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All tests were carried out under red light during the dark phase of the light:dark cycle. Tests were videotaped and coded prior to behavior scoring so that the investigator was blind to the treatment groups. To induce behavioral estrus, females were given a s.c. injection of estradiol benzoate (EB, 0.5 μg in 0.05 ml sesame oil, Sigma-Aldrich) 2 days before, and a s.c. injection of progesterone (P, 830 μg in 0.05 ml sesame oil, Sigma-Aldrich) 3–6 hours before testing (Bonthuis et al., 2011 (link)). At the end of each testing session, mice were returned to their home cage.

McCarthy E.A., Kunkhyen T., Korzan W.J., Naik A., Maqsudlu A., Cherry J.A, & Baum M.J. (2017). A comparison of the effects of male pheromone priming and optogenetic inhibition of accessory olfactory bulb forebrain inputs on the sexual behavior of estrous female mice. Hormones and behavior, 89, 104-112.

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Nicotine and Estrogen Receptor Modulation

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(-)Nicotine tartrate (MP Biomedicals) was dissolved in sterile 0.9% saline, and pH was adjusted to 7.2–7.4 using 1 m NaOH. E2 was purchased from Sigma-Aldrich and diluted to 3 μg/0.1 ml in sesame oil (Sigma-Aldrich). E1 was purchased from Sigma and was diluted to 10 μg/0.1 ml in 3 μg/0.1 ml E2 in sesame oil (Sigma-Aldrich). 6,7-dinitroquinoxaline-2,3(1H,4H)-dione (DNQX) was purchased from Tocris Biotechne and diluted to a stock concentration of 20 mm in dimethyl sulfoxide (DMSO). Before recordings, DNQX was diluted to a working concentration of 20 μm in recording aCSF.

Maher E.E., Kipp Z.A., Leyrer-Jackson J.M., Khatri S., Bondy E., Martinez G.J., Beckmann J.S., Hinds TD J.r., Bimonte-Nelson H.A, & Gipson C.D. (2022). Ovarian Hormones Regulate Nicotine Consumption and Accumbens Glutamatergic Plasticity in Female Rats. eNeuro, 9(3), ENEURO.0286-21.2022.

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Testosterone Modulation in Orchiectomized Rats

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Two to three months old male rats underwent bilateral ORX or sham operation under anesthesia with sodium pentobarbital (40 mg/kg; Koch-Light. Lab. Ltd., Colnbrook, Bucks, England). After 1-week recovery, the rats were divided into three and/or five groups: 1. sham-operated (Sham) rats were subcutaneously injected with sesame oil (Sigma-Aldrich, St Louis, MO, USA) as a control; 2. ORX rats were subcutaneously injected with sesame oil; 3. ORX rats were treated with testosterone propionate (TP, 2 mg/kg; Fluka, Buchs, Switzerland) (ORX + TP) via subcutaneous injection; 4. ORX rats were subcutaneously injected with TP combined with flutamide (Flu, 6 mg/kg; Sigma-Aldrich, St Louis, MO, USA) (ORX + TP + Flu); 5. ORX rats were subcutaneously injected with Flu (ORX + Flu) once daily for 7 days.

Lin P.H., Jian C.Y., Chou J.C., Chen C.W., Chen C.C., Soong C., Hu S., Lieu F.K., Wang P.S, & Wang S.W. (2016). Induction of renal senescence marker protein-30 (SMP30) expression by testosterone and its contribution to urinary calcium absorption in male rats. Scientific Reports, 6, 32085.

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Hormonal Regulation of Epididymal Function

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Mice were administered intraperitoneal injections of pregnant mare’s serum gonadotropin (PMSG) (10 IU/mouse, Sigma-Aldrich, USA) and human chorionic gonadotropin (hCG) (10 IU/mouse, Sigma-Aldrich, USA). Epididymis tissues were collected three days later. The epididymis tissues were divided into caput, corpus, and cauda parts and then analyzed for mRNA and protein expression levels. For castration, mice were anesthetized by intraperitoneal injection of a combination of Zoletil and Rompun. A dorsal incision was made through the mouse’s flank skin, and the testes were carefully excised, while the epididymis was preserved intact. One day post-castration, one group of mice received an intraperitoneal injection (100 μl/mouse) of vehicle (saline) alone. Another group was administered PMSG and hCG. Additionally, a separate group received a subcutaneous injection of vehicle (sesame oil) alone or testosterone (2 mM/100 μl, Sigma-Aldrich, USA) dissolved in sesame oil (Sigma-Aldrich, USA).

Kim S., Sun S., Kim M., Ha J., Seok E, & Yang H. (2023). NUCB2/nesfatin-1 suppresses the acrosome reaction in sperm within the mouse epididymis. Animal Cells and Systems, 27(1), 120-128.

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Ovariectomized Rat Estrogen Priming

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Male gonads were left intact and all females were subjected to OVX at least 7 d before each experiment. Rimadyl (4–5 mg/kg, s.c.) was given immediately after surgery for relief of postoperative pain. Females received either an injection of sesame oil (50 μl, s.c.; Sigma-Aldrich) or a priming dose (0.125 μg/50 μl sesame oil, s.c.) of E2 benzoate (Sigma-Aldrich) on the Friday morning following surgery. In addition, oil or a low (0.25 μg) and then a high (1.5 μg) dose of E2 benzoate was administered in the morning of the 2 d preceding experiments. Circulating levels of E2 were verified by the uterine weights (<25 mg for OVX and >95 mg for E2 treated) at the time of hypothalamic slice preparation (between 8:30 and 10:30 A.M.).

Stincic T.L., Grachev P., Bosch M.A., Rønnekleiv O.K, & Kelly M.J. (2018). Estradiol Drives the Anorexigenic Activity of Proopiomelanocortin Neurons in Female Mice. eNeuro, 5(4), ENEURO.0103-18.2018.

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Nicotine and Cannabidiol Co-Administration in Rats

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Nicotine hydrogen tartrate (Sigma) was dissolved in saline (nicotine dose 3.15 mg/kg/day) and brought to pH 7.4 with NaOH 1M. Osmotic minipumps (Alzet AP 2ML2) were filled with 2 ml of the nicotine solution the night before surgery was performed. Cannabidiol (Noramco Inc) was dissolved in sesame oil (Sigma) for daily subcutaneous injection at the doses 0, 7.5, 15, and 30 mg/kg. The higher dose of CBD used (30 mg/kg) translates to ~200 mg/kg in humans, which is recommended for CBD treatment of epilepsy (2018 (link)). Other groups have reported successful results of CBD at doses 15 and 30 mg/kg in anxiety like behavior in rats (Gonzalez-Cuevas, Martin-Fardon et al. 2018 (link)). Treatment with CBD was performed for 14 consecutive days using sesame oil (Sigma) as a control. CBD was administered daily starting 7 days after minipump implantation. On test days, CBD was administered 3 hours prior to the behavioral assessments.

Smith L.C., Tieu L., Suhandynata R.T., Boomhower B., Hoffman M., Sepulveda Y., Momper J.D., Fitzgerald R., Hanham K., Dowling J., Kallupi M, & George O. (2021). Cannabidiol reduces withdrawal symptoms in nicotine dependent rats. Psychopharmacology, 238(8), 2201-2211.

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