Methyl acetate

Manufactured by Thermo Fisher Scientific

Sourced in United States, Germany

Methyl acetate is a colorless, volatile liquid chemical compound. It has the chemical formula CH3COOCH3. Methyl acetate is primarily used as a solvent in various industrial and laboratory applications.

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5 protocols using methyl acetate

Colloidal Synthesis of Inorganic Nanomaterials

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Cadmium oxide (99.5%, powder), 2-ethylhexanoic acid (2-EHA, 99%), 1-octadecene (ODE, technical grade, 90%), oleylamine (OLA, technical grade, 70%), hexadecylamine (technical grade, 90%), trioctylamine (98%), trioctylphisphine (TOP, technical grade, 97%), selenium powder (powder, 100 mesh, 99.5%), zinc oxide (puriss, 99–100%), thiourea (TU, ACS Reagent,>99%), triethylene glycol dimethyl ether (TEGDME, 99%), hexadecylamine (technical grade, 90%), palmitic acid (BioXtra, ≥99%) zinc acetate dihydrate (ACS reagent, ≥99.0%), magnesium acetate tetrahydrate (ACS reagent, ≥98%), aluminium chloride hexahydrate (99%), gallium nitrate hydrate (99.9%), lithium chloride (anhydrous, ACS reagent, ≥99%), tetramethylammonium chloride (TMACl, reagent grade, ≥98%), potassium hydroxide (reagent grade, 90%), dimethyl sulfoxide (DMSO, anhydrous, ≥99.9%), and monoethanolamine (MEA, ACS reagent, ≥99.0%) were purchased from Sigma-Aldrich. n-Hexadecylphosphonic acid (97%) was from PlasmaChem GmbH. Methyl acetate (extra pure, 99%) was from Acros. Hexane, n-octane, toluene, acetone, methanol, ethanol, n-butanol, and isopropyl alcohol of spectroscopy grade were purchased from the local supplier Ekos-1. All reagents were used as received, without purification.

Alexandrov A., Zvaigzne M., Lypenko D., Nabiev I, & Samokhvalov P. (2020). Al-, Ga-, Mg-, or Li-doped zinc oxide nanoparticles as electron transport layers for quantum dot light-emitting diodes. Scientific Reports, 10, 7496.

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Comprehensive Lipid and Carbohydrate Analysis

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Hexane, methanol and dimethylformamide were HPLC grade (VWR Chemicals, West Chester, PA, USA). Lactic acid (≥90%) was also purchased from VWR. Sulfuric acid was obtained from Honeywell Fluka (Charlotte, NC, USA), while sodium methoxide and methyl acetate were obtained from Acros Organics (Geel, Belgium). GLC-Nestlé36 FAME mix was obtained from Nu-Chek Prep, Inc. (Elysian, MN, USA) and butterfat CRM-164 (EU Commission; Brussels, Belgium) from Fedelco Inc. (Madrid, Spain). Undecanoic acid (98.0%) was obtained from Alfa Aesar (Haverhill, MA, USA) while glyceryl tritridecanoate (>99.0%) was obtained from Larodan (Solna, Sweden). Galactose (≥98%) and acetic acid (100%) were obtained from Merck (Darmstadt, Germany). Sodium hydroxide was procured from LabChem (LaborSpirit Lda, Loures, Portugal) and phenolphthalein from José M. Vaz Pereira S.A. (Santarém, Portugal). Supelco 37 FAME mix, methyl tricosanoate (≥99.0%), α-lactose monohydrate (≥99%, total lactose basis), glucose (≥99.5%), Candida rugosa (CRL) type VII lipase and formic (≥95%), propionic (99%) and citric (99%) acids were purchased from Sigma-Aldrich (St. Louis, MO, USA). Pasteurized semi-skimmed cows’ milk and flaxseed oil (FSO) were bought in local markets (Porto, Portugal).

Fontes A.L., Pimentel L.L., Monteiro M.J., Domingues M.R., Rodríguez-Alcalá L.M, & Gomes A.M. (2023). Microbial Conjugated Linolenic Acid-Enriched Fermented Milk Using Lipase-Hydrolyzed Flaxseed Oil: Biochemical, Organoleptic and Storage Traits. Foods, 13(1), 21.

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Spectroscopic Analysis of Peptide Interactions

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Methyl acetate (acetic acid methyl ester) (MA) and methyl propionate (propanoic acid methyl ester) (MP) were purchased from Acros Organics (Fair Lawn, NJ) and used as received. Fmoc-L-Asp(Me)-OH was purchased from Chem-Impex International Inc. (Wood Dale, IL) and F-moc-L-Glu(Me)-OH was purchased from Santa Cruz Biotechnology Inc. Peptides were synthesized using standard Fmoc solid-phase methods on an PS3 peptide synthesizer from Protein Technologies (Tucson, AZ). All linear IR spectra, except the one for the Aβ-D_M dry film, which was obtained using a Horizon ATR unit from Harrick (Pleasantville, NY), were collected on a Nicolet Magna-IR 860 FTIR spectrometer at 1 cm^-1 resolution using a home-made CaF₂ sample holder. More details about the linear and 2D IR instrumentations can be found elsewhere^{[22 (link)]} or in the Supporting Information. AFM images were acquired on a Bruker Dimension Icon AFM (Santa Barbara, CA). The details of MD simulations and electric field calculations are given in the Supporting Information.

Pazos I.M., Ghosh A., Tucker M.J, & Gai F. (2014). Ester Carbonyl Vibration as a Sensitive Probe of Protein Local Electric Field. Angewandte Chemie (International ed. in English), 53(24), 6080-6084.

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Hybrid Perovskite Materials Synthesis

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Lead iodide (PbI₂) (purity 99%, Sigma Aldrich, St Louis, MO, USA), Cesium carbonate (Cs₂CO₃) (purity 99%, Thermo Fisher Scientific, Germany), Formamidinium bromide (FABr) (purity 99%, Greatcell Solar Materials, Queanbeyan, NSW, Australia), Phenylethylammonium iodide (PEAI) (purity 99%, Greatcell Solar Materials, Queanbeyan, NSW, Australia), Cyclohexylmethylammonium iodide (CHAI) (purity 99%, Greatcell Solar Materials, Queanbeyan, NSW, Australia), 4-Trifluoromethyl-benzylammonium iodide (TFBI) (purity 99%, Greatcell Solar Materials, Queanbeyan, NSW, Australia), Oleylamine (OLA) (Technical grade, purity 70%, Sigma Aldrich, Zwijndrecht, The Netherlands), Oleic acid (OA) (Technical grade, purity 90%, Sigma Aldrich, Zwijndrecht, The Netherlands), Octadecene (Technical grade, purity 90%, Sigma Aldrich, St. Louis, MO, USA), Hydriodic acid (57% in water, Alfa Aesar, Kandel, Germany), Isopropanol (IPA) (anhydrous, purity 99.5%, Sigma Aldrich, Steinheim am Albuch, Germany), Methyl acetate (Purity 99%, Thermo Fisher Scientific, Germany), Octane (Purity 99.8%, J T Baker Chemicals B V., Deventer, The Netherlands).

Narra S., Liao P.S., Bhosale S.S, & Diau E.W. (2023). Effect of Acidic Strength of Surface Ligands on the Carrier Relaxation Dynamics of Hybrid Perovskite Nanocrystals. Nanomaterials, 13(11), 1718.

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Solvent Extraction Purification Protocol

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M Ethyl acetate (MEAC, ≥99%), ethyl formate (ETFO, ≥98%), propyl acetate (PRAC, ≥99%), ethyl propionate (ETPR, ≥99%), ethyl butyrate (ETBU, ≥99%), and hexyl acetate (HEAC ≥ 99%) were purchased from Thermo Fisher Scientific. Ethyl acetate (ETAC, ≥99.8%) was from Honeywell, butyl acetate (BUAC, ≥99.5%) from Emsure and pentyl acetate (PEAC, ≥99%) from Supelco. Polyoxyethylene (4) lauryl ether (Brij 30, ≥99%, max. 1% H₂O) was acquired from Acros Organics. Isopropanol (C3-ol, anh., ≥99.5%), chloroform (≥99.9%, with amylenes as stabilizer), sodium chloride (≥99%), sodium hydroxide (≥97%), phosphoric acid (85% in water), amitriptyline hydrochloride (AMI, ≥98%), doxepin hydrochloride (DOX, ≥98%, ~85% E isomer and 15% Z isomer), imipramine hydrochloride (IMI, ≥99%), and clomipramine hydrochloride (CLO, ≥98%) were from Sigma Aldrich. Mirtazapine (MIR, ≥98%) was purchased from Thermo Fisher Scientific. Helium 6.0 (≥99.9999%) was purchased from Linde GmbH. Doubly distilled water from an in-house distillation setup was used in all experiments. Brij 30 was dried at 105 °C overnight before use. All other reagents were used as received.

Racovita R.C., Ciuca M.D., Catana D., Comanescu C, & Ciocirlan O. (2023). Microemulsions of Nonionic Surfactant with Water and Various Homologous Esters: Preparation, Phase Transitions, Physical Property Measurements, and Application for Extraction of Tricyclic Antidepressant Drugs from Aqueous Media. Nanomaterials, 13(16), 2311.

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