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Anti egfr rabbit polyclonal antibody clone 1005

Manufactured by Santa Cruz Biotechnology
Sourced in United States

The Anti-EGFR rabbit polyclonal antibody (clone 1005) is a laboratory research tool used to detect the epidermal growth factor receptor (EGFR) protein in various experimental applications. This antibody is produced in rabbits and recognizes the EGFR protein.

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2 protocols using anti egfr rabbit polyclonal antibody clone 1005

1

Immunohistochemical Staining of Tissue

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IHC staining of paraffin-embedded tissue sections was carried out using the Envision System (Dako, Glostrup, Denmark) as described previously13 (link). The primary antibodies used were as follows: anti-FSCN1 mouse monoclonal antibody (clone 55k-2, diluted at 1:100, Santa Cruz Biotechnology, Santa Cruz, USA); anti-EGFR rabbit polyclonal antibody (clone 1005, diluted at 1:100, Santa Cruz Biotechnology); HercepTest (Dako); ready-to-use anti-ER rabbit monoclonal antibody (clone SP1, Dako); ready-to-use anti-PR mouse monoclonal antibody (clone PR636, Dako).
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2

Immunohistochemical Analysis of Breast Cancer Biomarkers

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We followed the methods of Wang et al. [18 (link)]. The primary antibodies consisted of anti-resistin mouse monoclonal antibody (clone C-10, diluted at 1 : 25; Santa Cruz Biotechnology, Santa Cruz, USA), anti-EGFR rabbit polyclonal antibody (clone 1005, diluted at 1 : 100; Santa Cruz Biotechnology), anti-ERK1/2 rabbit monoclonal antibody (clone EPR18444, diluted at 1 : 1000; Abcam, Cambridge, England), ready-to-use anti-ER rabbit monoclonal antibody (clone SP1, Dako), ready-to-use anti-PR mouse monoclonal antibody (clone PgR636, Dako), HercepTest (Dako), and ready-to-use anti-Ki-67 mouse monoclonal antibody (clone MIB-1, Dako).
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