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Human specific elisa kit

Manufactured by Thermo Fisher Scientific
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The human-specific ELISA kits are laboratory equipment designed to detect and measure specific proteins or analytes in human samples. These kits use the enzyme-linked immunosorbent assay (ELISA) technique to quantify the target analyte. The core function of these kits is to provide a standardized and reliable method for analyzing the presence and concentration of target molecules in human-derived samples.

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Lab products found in correlation

Protease inhibitors cocktail, by Merck Group (1 mentions) Centrifuge tube, by Eppendorf (1 mentions) Protease inhibitor, by Merck Group (1 mentions)

Close spelling variants of this product

Human specific ELISA Specific ELISA kits Specific ELISA ELISAs

4 protocols using human specific elisa kit

1

Enzyme-Linked Immunosorbent Assay Protocol

Cited 1 time
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Human-specific ELISA kits (Invitrogen) were used, as we described27 (link).
Zhao Z., Sagare A.P., Ma Q., Halliday M.R., Kong P., Kisler K., Winkler E.A., Ramanathan A., Kanekiyo T., Bu G., Owens N.C., Rege S.V., Si G., Ahuja A., Zhu D., Miller C.A., Schneider J.A., Maeda M., Maeda T., Sugawara T., Ichida J.K, & Zlokovic B.V. (2015). Central role for PICALM in amyloid–β blood–brain barrier transcytosis and clearance. Nature neuroscience, 18(7), 978-987.
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2

Enzyme-Linked Immunosorbent Assay Protocol

Cited 1 time
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Human-specific ELISA kits (Invitrogen) were used, as we described27 (link).
Zhao Z., Sagare A.P., Ma Q., Halliday M.R., Kong P., Kisler K., Winkler E.A., Ramanathan A., Kanekiyo T., Bu G., Owens N.C., Rege S.V., Si G., Ahuja A., Zhu D., Miller C.A., Schneider J.A., Maeda M., Maeda T., Sugawara T., Ichida J.K, & Zlokovic B.V. (2015). Central role for PICALM in amyloid–β blood–brain barrier transcytosis and clearance. Nature neuroscience, 18(7), 978-987.
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3

Quantifying IL-1β in Microparticle Samples

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A human-specific ELISA Kit (eBioscience, San Diego, CA) was used to evaluate the concentration of IL-1β in MPs samples following the manufacturer’s instructions. MPs were first prepared as described for flow cytometry but then centrifuged at 100,000 g for 60 min, and the MPs pellet lysed with 0.3 ml lysis buffer (20 mM Tris, 150 mM NaCl, 1% Nonidet P-40, 0.5% sodium deoxycholate, 1 mM EDTA and 0.1% SDS (pH 7.5) with protease inhibitors cocktail (Sigma)). The protein content of the sample was measured, diluted to 5 mg/ml, and 20 µg protein used for analysis. Some MPs samples were also subjected to Western blotting. Following the standard preparation of 15,000-g plasma supernatants, MPs were counted and a volume containing exactly 10,000 MPs was centrifuged at 21,000-g to sediment the MPs. The MPs pellets and supernatant from the same samples were then blotted to discern if IL-1β was within or outside MPs.
Brett K.D., Nugent N.Z., Fraser N.K., Bhopale V.M., Yang M, & Thom S.R. (2019). Microparticle and interleukin-1β production with human simulated compressed air diving. Scientific Reports, 9, 13320.
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4

Quantification of IL-1β in LPS-stimulated HDPFs

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The HDPFs were counted and plated on 6-well plates (1 × 106 cells per well) in three wells per group. After 24 h, α-MEM was replaced with various media, as described below. Cells were exposed to ATP (5 mM) for 2 h and then pretreated with specific inhibitors for the indicated time, followed by LPS exposure at 10 μg/ml for 6 or 12 h. After incubation, the media were removed and the cells were washed with PBS at 4 °C. During protein extraction, the plates were kept on ice to avoid the denaturation of the cytokines. Each well was filled with 100 μl lysis buffer, supplemented with 10 μl protease inhibitor (Sigma, US) for 15 min; subsequently, the cells were collected by vigorous scraping by using a cell scraper, transferred into Eppendorf centrifuge tubes and centrifuged at 4 °C at 12,000g for 15 min. The supernatant was collected after centrifugation. The amount of IL-1β protein in the culture medium was quantified by using a commercially available human-specific ELISA kit, following the manufacturer’s instructions (eBioscience, San Diego, Calif., USA).
Jiang W., Lv H., Wang H., Wang D., Sun S., Jia Q., Wang P., Song B, & Ni L. (2015). Activation of the NLRP3/caspase-1 inflammasome in human dental pulp tissue and human dental pulp fibroblasts. Cell and Tissue Research, 361(2), 541-555.
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