Pe anti igm eb121 15f9 apc anti cd93 aa4.1 fitc anti f4 80 bm8

Manufactured by Thermo Fisher Scientific

PE-anti-IgM (eB121-15F9) is a fluorescently-labeled antibody specific for the immunoglobulin M (IgM) surface marker. APC anti-CD93 (AA4.1) is a fluorescently-labeled antibody that recognizes the CD93 cell surface antigen. FITC-anti-F4/80 (BM8) is a fluorescently-labeled antibody that binds to the F4/80 antigen, which is expressed on macrophages.

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Lab products found in correlation

Lsr 2 flow cytometer, by BD (2 mentions) Facsdiva software, by BD (2 mentions) Anti pd 1 pe, by BioLegend (2 mentions) Anti cd95 pecy7, by BioLegend (2 mentions) Anti cd4 alexa fluor 700, by BioLegend (2 mentions) Pecy7 anti mhc 2, by BioLegend (2 mentions) Fitc peanutagglutinin pna, by Vector Laboratories (2 mentions) Cy5 anti cd86, by BioLegend (2 mentions) Apc anti cd24 hsa, by BioLegend (2 mentions) Biotin anti ly5.1 bp 1 6c3 fitc anti cd23 b3b4 pe cy5 streptavidin sa, by BioLegend (2 mentions) Anti cd25 apc cy7, by BioLegend (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions)

Close spelling variants of this product

Anti-F4/80 (122 citations) F4/80-PE (78 citations) F4/80 (BM8, (74 citations) F4/80-APC (72 citations) F4/80-FITC (40 citations) Anti-F4/80-PE (38 citations) Anti-F4/80-APC (35 citations) Anti-F4/80-FITC (30 citations) Anti-F4/80 (BM8; (11 citations) CD93 (AA4.1) (8 citations)

2 protocols using pe anti igm eb121 15f9 apc anti cd93 aa4.1 fitc anti f4 80 bm8

Comprehensive Immunophenotyping of Mouse Immune Cells

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The following antibodies were utilized for flow cytometric analysis of mouse splenocytes or bone marrow cells: PacBlue-anti-B220 (RA3-6B2); Alexa Fluor 700-anti-CD4 (RM4-5); PE-anti-PD-1 (29F.1A12); PerCP-Cy5.5-anti-CD69 (H1.2F3); APC-Cy7- anti-CD25 (PC61); Cy5-anti-CD86 (GL1); PeCy7-anti-CD95 (FAS, Jo2); PeCy7-anti-MHC-II (M5/114.15.2); APC-anti-CD24 (HSA) (M1/69); Biotin-anti-Ly5.1 (BP-1) (6C3); FITC-anti-CD23 (B3B4); PE-Cy5-streptavidin (SA) were from purchased from BioLegend, San Diego, CA. Biotin-anti-CXCR5 (2G8) from BD Pharmingen, San Diego, CA. FITC-peanutagglutinin (PNA) from Vector Labs, Burlingame, CA. PE-anti-IgM (eB121-15F9); APC anti-CD93 (AA4.1); FITC-anti-F4/80 (BM8) from eBiosciences, San Diego, CA. The following antibodies were utilized for phosphoflow analysis of purified mouse B cells: PE-anti-Btk (pY223)/ItK (pY180) (N35-86); Alexa Fluor 647-anti-ERK1/2 (pT202/pY204) (20A); Pacific Blue-anti-p38 MAPK (pT180/pY182); Alexa Fluor 488-anti-Syk (pY348) (I120-722). Stained cells were analyzed using the BD LSR II flow cytometer (BD Biosciences, Franklin lakes, NJ). Data were acquired using FACSDiva software (BD Biosciences, San Jose, CA) and analyzed using FlowJo software (Tree Star, San Carlos, CA).

Wong E.B., Soni C., Chan A.Y., Domeier P.P., S.h.w.e.t.a.n.k., Abraham T., Limaye N., Khan T.N., Elias M.J., Chodisetti S.B., Wakeland E.K, & Rahman Z.S. (2015). B cell-intrinsic CD84 and Ly108 maintain germinal center B cell tolerance. Journal of immunology (Baltimore, Md. : 1950), 194(9), 4130-4143.

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Flow Cytometry of Mouse Immune Cells

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The following antibodies were utilized for flow cytometric analysis of mouse splenocytes or bone marrow cells: PacBlue-anti-B220 (RA3-6B2); Alexa Fluor 700-anti-CD4 (RM4-5); PE-anti-PD-1 (29F.1A12); PerCP-Cy5.5-anti-CD69 (H1.2F3); APC-anti-TCR Vα2 (B20.1); APC-Cy7- anti-CD25 (PC61); Cy5-anti-CD86 (GL1); PeCy7-anti-CD95 (FAS, Jo2); PeCy7-anti-MHC-II (M5/114.15.2); APC-anti-CD24 (HSA) (M1/69); Biotin-anti-Ly5.1 (BP-1) (6C3); FITC-anti-CD23 (B3B4); PE-Cy5-streptavidin (SA) were from purchased from BioLegend, San Diego, CA. Biotin-anti-CXCR5 (2G8); FITC-anti-CD11c (HL3); FITC-anti-CD43 (S7) from BD Pharmingen, San Diego, CA. FITC-peanut-agglutinin (PNA) from Vector Labs, Burlingame, CA. PE-anti-IgM (eB121-15F9); APC-anti CD93 (AA4.1); FITC-anti-F4/80 (BM8) from eBiosciences, San Diego, CA. Stained cells were analyzed using the BD LSR II flow cytometer (BD Biosciences, Franklin lakes, NJ). Data were acquired using FACSDiva software (BD Biosciences, San Jose, CA) and analyzed using FlowJo software (Tree Star, San Carlos, CA). Dead cells were quantified by flow cytometry using 4′,6-diamidino-2-phenylindole (DAPI) (Sigma-Aldrich, St. Louis, MO).

Soni C., Wong E.B., Domeier P.P., Khan T.N., Satoh T., Akira S, & Rahman Z.S. (2014). B cell-intrinsic TLR7 signaling is essential for the development of spontaneous germinal centers. Journal of immunology (Baltimore, Md. : 1950), 193(9), 4400-4414.

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