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Colorview iiiu

Manufactured by Olympus
Sourced in Japan

The Colorview IIIu is a high-performance color camera system designed for a wide range of microscopy applications. It features a state-of-the-art CMOS sensor that delivers exceptional image quality and color accuracy. The camera supports various resolutions and frame rates, allowing users to capture detailed images and videos. The Colorview IIIu is compatible with a variety of microscopes and can be controlled through intuitive software for seamless integration into research and analysis workflows.

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7 protocols using colorview iiiu

1

Microscopy Imaging Techniques

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ISH images were taken by a light microscope (BX61, Olympus) with a CCD camera (ColorView IIIu, Soft Imaging System, Olympus) and processed by Adobe Photoshop CS3. Fluorescence Z-stacks photos were taken by a confocal laser scanning microscope (Leica SP5, Leica Microsystems) and processed by Imaris 7.6.3 (Bitplane, Zurich, Switzerland).
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2

Optical Microspectroscopy Analysis of Extrudates

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Optical microspectroscopy studies were performed in reflectance mode by using an Olympus BX41M upright research microscope, equipped with a 50×0.5 NA objective lens. Illumination of the sample was performed with a 30 W halogen lamp. The microscopy setup was equipped with a 50/50 double‐viewport tube, which accommodated a CCD video camera (ColorView IIIu, Soft Imaging System GmbH) and an optical fiber mount. The microscope was connected to a CCD Visible spectrometer (AvaSpec‐2048TEC, Avantes) by a 200 μm core fiber. UV/Vis spectroscopy measurements were performed by using an open in situ cell (Linkam Scientific Instruments, FTIR 600) equipped with a temperature controller (Linkam Scientific Instruments TMS 94). All extrudate samples used in the experiment were of similar dimensions (5×1.5 mm), with spectra collected from approximately 5×5 μm areas of each extrudate. Each sample was impregnated with the desired probe molecule solution (5 μL) at 30 °C, before applying a temperature ramp (at 30 °C min−1) to 120 °C. Optical absorption spectra were recorded every 10 s for a total of 1000 s.
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3

In situ UV-Vis Micro-Spectroscopy of Zeolite Catalysts

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The UV/Vis micro-spectroscopy measurements were performed with an Olympus BX41 upright microscope using a 50×0.5 NA high working-distance microscope objective lens. A 75 W tungsten lamp was used for illumination. In addition, the microscope has a 50/50 double viewpoint tube, which accommodates a CCD video camera (ColorView IIIu, Soft Imaging System GmbH) and an optical fibre mount. A 200 μm core fibre connects the microscope to a CCD UV/Vis spectrometer (AvaSpec-2048TEC, Avantes BV). The large zeolite H-ZSM-5 crystals were loaded into an in situ cell (Linkam FTIR 600) equipped with a temperature controller (Linkam TMS 93). The crystals were placed in a flow of 10 mL min−1 O2/40 mL min−1 N2 and heated to 500 °C at a rate of 15 min−1, the crystals were kept at this temperature for 1 h before cooling to required reaction temperature (at a rate of 15 min−1). Once the reaction temperature was achieved, the flow was changed to 50 mL min−1 N2 only. Subsequently, the N2 flow was diverted through a bubbler containing methanol or ethanol for the desired reaction time.
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4

Pollen and Spore Microscopy Imaging

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Photographs of pollen and spores were obtained by a digital camera Olympus Colorview IIIu attached on the Olympus BX51 light microscope, after being mounted in 2.5% potassium hydroxide (KOH) solution. Pollen grains were photographed through a UPlanFl 40× objective, and spores through a UPlanSApo 100× oil objective. Pollen grains of Abies cephalonica and spores of Geastrum triplex, Scleroderma areolatum, and S. citrinum are shown with composite extended depth of field images made from stack of images by CombineZP software.
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5

Histological Examination of Joint Tissues

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Formalin-fixed sections of joints were embedded in paraffin blocks, sliced and stained with Delafield’s haematoxylin–water eosin. Preparations were viewed and evaluated using a BX53 Olympus microscope. Digital photos were taken with an Olympus Colorview IIIu camera.
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6

Paraffin Sectioning and H&E Staining

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Tissue samples were embedded in paraffin blocks (Merck, Darmstadt, Germany) which were cut on fully automated Rotary Microtome (Leica RM 2255, Nussloch, Germany) into 4-µm-thick paraffin sections. Subsequently, sections were stained with Mayer's hematoxylin and eosin (H&E) (Bio-Optica Milano, Italy) and were analyzed with the use of a light microscope BX41 (Olympus, Tokyo, Japan), connected to a digital camera Colorview IIIu (Olympus).
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7

Histological Evaluation of Muscle Inflammation

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Tissue samples were fixed in 4% buffered formalin, dehydrated, and embedded in paraffin. For every studied sample, four 6-mm thick paraffin sections stained with HE were prepared. The severity of inflammation was evaluated under BX53 light microscope (Olympus, Tokyo, Japan) according to the scale of Godsel et al: absent (1 point; no inflammatory infiltration), mild (2 points; 10% of inflammatory infiltration in muscle tissue), moderate (3 points; >10% and 25% of inflammatory infiltration in muscle tissue), and severe (4 points; >25% of inflammatory infiltration in muscle tissue). 25 Images were made using BX41 microscope (Olympus) connected with camera Colorview IIIu (Olympus).
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