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6 well ultra low cluster plates

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The 6-well ultra-low cluster plates are a laboratory equipment product designed for cell culture applications. The plates feature a hydrophobic surface that promotes the formation of 3D cell aggregates, also known as spheroids or organoids. This specialized surface minimizes cell attachment and encourages the cells to self-assemble into cellular clusters, which is useful for various research and development purposes.

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Lab products found in correlation

Insulin, by Merck Group (9 mentions) Epidermal growth factor (egf), by Thermo Fisher Scientific (8 mentions) Dmem f12 serum free medium, by Thermo Fisher Scientific (7 mentions) Bovine serum albumin (bsa), by Merck Group (7 mentions) Basic fibroblast growth factor (bfgf), by Thermo Fisher Scientific (6 mentions) Epidermal growth factor (egf), by Merck Group (4 mentions) Basic fibroblast growth factor (bfgf), by Merck Group (3 mentions) Heparin, by Merck Group (2 mentions) Lsrii flow cytometer, by BD (1 mentions) Basic fibroblast growth factor bfgf, by Merck Group (1 mentions) Aldefluor reagent, by STEMCELL (1 mentions) 24 well ultra low cluster plates, by Corning (1 mentions) Human egf, by Merck Group (1 mentions) Human bfgf, by Merck Group (1 mentions) B27 supplement, by Thermo Fisher Scientific (1 mentions) Dmem f12 1 1 medium, by Thermo Fisher Scientific (1 mentions) Heparin, by Solarbio (1 mentions) 24 well plastic plates, by Corning (1 mentions) Transwell insert, by Corning (1 mentions) Dmem f12 medium, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

6-well plates (1462 citations) Ultra Low Cluster plates (34 citations) 6-well ultra-low attachment cluster plates (5 citations) 6-well cluster plates (3 citations) Ultra Low plates (3 citations)

17 protocols using 6 well ultra low cluster plates

1

Cell Spheroid Formation Assay

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Cells (500 cells/well) were seeded into 6-well ultra low cluster plates (Corning) and cultured as previously described.45 (link) After 10–12 days, the number of cell spheroids (tight, spherical, non-adherent masses >50 μm in diameter) were counted, and images of the spheroids were scored under an inverse microscope (spheroid formation efficiency = colonies/input cells × 100%).
Ruan X., Liu A., Zhong M., Wei J., Zhang W., Rong Y., Liu W., Li M., Qing X., Chen G., Li R., Liao Y., Liu Q., Zhang X., Ren D, & Wang Y. (2019). Silencing LGR6 Attenuates Stemness and Chemoresistance via Inhibiting Wnt/β-Catenin Signaling in Ovarian Cancer. Molecular Therapy Oncolytics, 14, 94-106.
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2

Cell Spheroid Formation Assay

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Cells (500 cells/well) were seeded into 6-well Ultra Low Cluster plates (Corning) and cultured as previously described [36 (link)]. After 10–12 days, the number of cell spheroids (tight, spherical, non-adherent masses > 50 μm in diameter) were counted, and images of the spheroids were scored under an inverse microscope (spheroids formation efficiency = colonies/input cells× 100%).
Wang X., Sun D., Tai J., Chen S., Yu M., Ren D, & Wang L. (2018). TFAP2C promotes stemness and chemotherapeutic resistance in colorectal cancer via inactivating hippo signaling pathway. Journal of Experimental & Clinical Cancer Research : CR, 37, 27.
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3

Isolation and Characterization of Cancer Stem Cells

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The LSR II flow cytometer (BD Pharmingen, San Diego, CA, USA) was used to analyze and separate CSCs based on cell labeling and fluorescence-activated sorting. The activated ALDEFLUOR™ reagent and diethylaminobenzaldehyde purchased from Stemcell Technologies, Inc. (Vancouver, BC, Canada) were used to isolate aldehyde dehydrogenase (ALDH)+ cells (21 (link)–23 (link)). The ratios of ALDH1+ stem cells in different groups were analyzed. The ratios of aldH 1+ stem cells in different groups were analyzed. For sphere forming assay, CSCs (1000cells per/ well) were seeded in 6-well ultra-low cluster plates (Corning Inc., Corning, NY) for one week to obtain first generation after being given different treatment. Spheres were cultured in DMEM/F12 serum-free medium (Invitrogen) supplemented with 2% B27 (BD Biosciences, CA), 20ng/ml epidermal growth factor (EGF, Sigma), 20ng/ml basic fibroblast growth factor (bFGF, Sigma), 0.4% BSA and 4 μg/ml insulin (Sigma). The number of spheres was counted under a microscope.
Sun X., Xu C., Xiao G., Meng J., Wang J., Tang S.C., Qin S., Du N., Li G., Ren H, & Liu D. (2018). Breast cancer stem-like cells are sensitized to tamoxifen induction of self-renewal inhibition with enforced Let-7c dependent on Wnt blocking. International Journal of Molecular Medicine, 41(4), 1967-1975.
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4

Seeding and Culturing Cell Spheres

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1 × 103 cells were seeded in 6-well ultra low cluster plates (Corning, NY) and about 10 cells were seeded in 24-well ultra low cluster plates (Corning, NY) for 15 days. Spheres were cultured in DMEM/F12 serum-free medium (Invitrogen, Grand Island, NY) supplemented with 2% B27 (Invitrogen, Grand Island, NY), 20 ng/ml of EGF, and 20 ng/ml of bFGF (PeproTech, Offenbach, Germany), 0.4% bovine serum albumin (BSA) (Sigma, St. Louis, MO, USA), and 5 μg/ml insulin.
Fan D., Ren B., Yang X., Liu J, & Zhang Z. (2016). Upregulation of miR-501-5p activates the wnt/β-catenin signaling pathway and enhances stem cell-like phenotype in gastric cancer. Journal of Experimental & Clinical Cancer Research : CR, 35, 177.
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5

Sphere Culture of Tumor Cells

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In total, 500 cells were seeded in 6-well ultra-low cluster plates (Corning Inc., Corning, NY) for 10 days. Spheres were cultured in DMEM/F12 serum-free medium (Invitrogen) supplemented with 2% B27 (BD Pharmingen, Carlsbad, CA), 20 ng ml−1 EGF, 20 ng ml−1 bFGF, 0.4% BSA, and 5 μg ml−1 insulin (Sigma).
Fang L., Cai J., Chen B., Wu S., Li R., Xu X., Yang Y., Guan H., Zhu X., Zhang L., Yuan J., Wu J, & Li M. (2015). Aberrantly expressed miR-582-3p maintains lung cancer stem cell-like traits by activating Wnt/β-catenin signalling. Nature Communications, 6, 8640.
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6

Sphere Formation Assay for Stem Cells

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Cells (500/well) were seeded into 6-well ultra-low cluster plates (Corning, NY) and cultured in DMEM/F12 serum-free medium (Invitrogen) supplemented with 2% B27 (Invitrogen), 20 ng/ml EGF (PeproTech, Rocky Hill, NJ, USA), 20 ng/ml bFGF (PeproTech), 0.4% BSA (Sigma-Aldrich), and 5 μg/ml insulin (Sigma-Aldrich). After 10 days, the number of spheres was counted, and images of the spheres were photographed.
Li Q., Ye L., Guo W., Wang M., Huang S, & Peng X. (2017). PHF21B overexpression promotes cancer stem cell-like traits in prostate cancer cells by activating the Wnt/β-catenin signaling pathway. Journal of Experimental & Clinical Cancer Research : CR, 36, 85.
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7

Spheroid Formation and Counting Protocol

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The cells were cultured for 10 days as previously stated.28 In brief, 500 cells/wells were seeded into 6‐well ultra‐low cluster plates (Corning, NY, USA). Cells were cultured in DMEM, including 2% B27 (Invitrogen), 20 ng/mL human EGF (Sigma‐Aldrich, St. Louis, MO, USA), 20 ng/mL human bFGF (Sigma‐Aldrich), 5 μg/mL insulin (Sigma‐Aldrich) and .4% BSA (Sigma‐Aldrich). After 10 days of culture, an optical microscope was used to counted microspheres with larger than 100 μm in diameter.
Yao J., Wang X., Yang J., Yang Z, & Zhang Z. (2023). SCF‐FBXL8 contributes to liver metastasis and stem‐cell‐like features in colorectal cancer cells by mediating ubiquitination and degradation of TP53. Clinical and Translational Medicine, 13(3), e1208.
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8

Seeding and Culturing Tumor Spheres

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In total, 1×103 cells were seeded in 6-well ultra-low cluster plates (Corning, NY, USA) for 10 days. Spheres were cultured in RPMI 1640 serum-free medium (Invitrogen, CA, USA) supplemented with 2% B27 (Invitrogen, CA, USA), 20 ng/ml EGF, 20 ng/ml bFGF, 0.4% BSA, and 5 μg/ml insulin (Sigma, Beijing, China).
Zhang W., Kong L., Zhu H., Sun D., Han Q., Yan B., Cui Z., Zhang W., Zhang S., Kang X., Dai G., Qian N, & Yan W. (2022). Retinoic Acid-Induced 2 (RAI2) Is a Novel Antagonist of Wnt/β-Catenin Signaling Pathway and Potential Biomarker of Chemosensitivity in Colorectal Cancer. Frontiers in Oncology, 12, 805290.
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9

Sphere Culture Protocol for Cancer Research

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Six hundred stable cell lines were seeded in 6-well ultra-low cluster plates (Corning Incorporated, Corning, NY, USA) for 10 days. Spheres were cultured in DMEM/F12 serum-free medium (Thermo Fisher Scientific) supplemented with 2% B27 (Thermo Fisher Scientific), 20 ng/mL EGF, and 20 ng/mL bFGF (PeproTech, Offenbach, Germany), 5 μg/mL insulin, and 0.4% BSA (Sigma-Aldrich Co.).
Zhou L., Deng Z.Z., Li H.Y., Jiang N., Wei Z.S., Hong M.F., Wang J.H., Zhang M.X., Shi Y.H., Lu Z.Q, & Huang X.M. (2019). Overexpression of PRR11 promotes tumorigenic capability and is associated with progression in esophageal squamous cell carcinoma. OncoTargets and therapy, 12, 2677-2693.
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10

Seeding and Culturing Spheres

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Five hundred cells were seeded in 6-well ultra-low cluster plates (Corning, NY, USA) for 10 days. Spheres were cultured in DMEM/F12 serum-free medium (Invitrogen, Grand Island, NY, USA) supplemented with 2% B27 (Invitrogen, Grand Island, NY, USA), 20 ng/ml epidermal growth factor, 20 ng/ml basic fibroblast growth factor (PeproTech, Offenbach, Germany), 5 μg/ml insulin and 0.4% bovine serum albumin (Sigma).
Zhang T., Huang Y., Liu W., Meng W., Zhao H., Yang Q., Gu S.J., Xiao C.C., Jia C.C., Zhang B., Zou Y., Li H.P, & Fu B.S. (2017). Overexpression of zinc finger protein 687 enhances tumorigenic capability and promotes recurrence of hepatocellular carcinoma. Oncogenesis, 6(7), e363-.
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