Nicolet is10 ftir

Manufactured by Thermo Fisher Scientific

Sourced in United States, China

The Nicolet iS10 FTIR is a Fourier Transform Infrared (FTIR) spectrometer designed for analytical laboratory use. It provides infrared spectroscopy capabilities for the identification and analysis of a wide range of materials and substances. The core function of the Nicolet iS10 FTIR is to measure the absorption and transmission of infrared light by samples, generating spectral data that can be used for qualitative and quantitative analysis.

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Lab products found in correlation

Ascend 600 mhz spectrometer, by Bruker (2 mentions) Refractive index detector, by Wyatt Technology (2 mentions) Microsorb 100 5 c18 column, by Agilent Technologies (2 mentions) Prostar 325 uv vis, by Agilent Technologies (2 mentions) Omnic software version 8, by Thermo Fisher Scientific (2 mentions) Smart itr accessory, by Thermo Fisher Scientific (2 mentions) S 2700 sem, by Hitachi (2 mentions) Avance 400 mhz nmr spectrometer, by Bruker (2 mentions) Uv 2550 spectrometer, by Shimadzu (2 mentions) 400 mhz spectrometer, by Bruker (2 mentions) K alpha spectrometer, by Thermo Fisher Scientific (2 mentions) Arx 400 mhz spectrometer, by Bruker (1 mentions) D2 phaser, by Bruker (1 mentions) Discovery hybrid rheometer 1, by TA Instruments (1 mentions) Thermo u3000 hplc system, by Thermo Fisher Scientific (1 mentions) Z gradient probe, by Bruker (1 mentions) Ultima 4, by Rigaku (1 mentions) Jsm 7600f, by JEOL (1 mentions) Rf 5301pc, by Shimadzu (1 mentions) Avance drx 800, by Bruker (1 mentions)

Close spelling variants of this product

Nicolet iS10 (648 citations) Nicolet iS10 FTIR spectrophotometer (47 citations) FTIR-Nicolet iS10 spectrometer (5 citations) Nicolet iS10 model FTIR spectrometer (4 citations) Nicolet iS10 FTIR apparatus (3 citations) NICOLET iS10 Thermo Scientific FTIR spectrometer (3 citations) Nicolet iS10 ATR-FTIR spectrophotometer (2 citations) Nicolet iS10 Fourier Transform Infrared (FTIR) spectrophotometer (2 citations) Nicolet iS10 ATR-FTIR system (2 citations)

71 protocols using nicolet is10 ftir

Characterization of Methcathinone Analogs

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A Thomas-Hoover melting point apparatus was used for the determination of melting points and the values are uncorrected. A Bruker ARX 400 MHz spectrometer was used for recording 1H NMR spectra with tetramethylsilane (TMS) as an internal standard and IR spectra were obtained using a Thermo Nicolet iS10 FT-IR. Elemental analysis was performed by Atlantic Microlab Inc. (Norcross, GA) and observed values were within 0.4 % of theory. A CombiFlash Companion/TS (Telodyne Isco Inc., Lincoln, NE) was used for automated flash chromatography. Silica Gel (230–400 mesh) was used as adsorbent, and RediSep Rf Normal-phase Silica Flash Columns (Teledyne Isco Inc., Lincoln, NE) were used as the stationary phase. Thin-layer chromatography on silica gel GHLF plates (250 μ, 2.5 × 10 cm; Analtech Inc., Newark, DE) was used for monitoring reactions. Note: Although all compounds described here are methcathinone analogs, this section identifies them by their correct chemical names.

Walther D., Shalabi A.R., Baumann M.H, & Glennon R.A. (2018). Systematic structure-activity studies on selected 2-, 3-, and 4-monosubstituted synthetic methcathinone analogs as monoamine transporter releasing agents. ACS chemical neuroscience, 10(1), 740-745.

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Comprehensive Analysis of Hybrid Materials

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Several analysis techniques were used to study the atomic (MAS NMR), molecular (FTIR and XRD), nano- (SEM) and macro-scale (SIMS) structure and composition of the hybrids. All ²⁹Si solid-state magic-angle spinning nuclear magnetic resonance (MAS NMR) data were acquired at 7.05 T on a Varian InfinityPlus 300 spectrometer operating at a ²⁹Si Lamor frequency of 59.62 MHz. These measurements were undertaken by using a Varian 7.5 mm probe spinning at 5 kHz. A 5 μs excitation pulse (flip angle ∼π/4) was applied with a 240 s recycle delay to ensure complete relaxation was obtained for each measurement. All ²⁹Si MAS NMR data were referenced to the IUPAC recommended primary reference of TMS (δ=0 ppm) by a secondary reference of kaolinite located at δ=−92 ppm. To quantify the Tⁿ and Qⁿ distribution of the Si speciation, each resonance in the ²⁹Si MAS NMR data was simulated with a Gaussian peak shape by using the Origin 8.5 Pro software package (Origin 8.5 Pro, OriginLab, Northampton). X-ray diffraction was performed on a Bruker D2 Phaser, using a step-scanning method with Cu_Kα radiation at 30 kV and 10 mA with a count rate of 0.25 s per step, from 2θ values of 10° to 70°. Attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR) was used to study the chemical structures of the hybrids. Samples were analysed on a Thermo Scientific Nicolet iS10 FTIR.

Poologasundarampillai G., Yu B., Tsigkou O., Wang D., Romer F., Bhakhri V., Giuliani F., Stevens M.M., McPhail D.S., Smith M.E., Hanna J.V, & Jones J.R. (2014). Poly(γ-glutamic acid)/Silica Hybrids with Calcium Incorporated in the Silica Network by Use of a Calcium Alkoxide Precursor. Chemistry (Weinheim an Der Bergstrasse, Germany), 20(26), 8149-8160.

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FTIR Analysis of Protein Structure

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The secondary structures of samples were measured using a Nicolet Is10 FTIR Fourier transform infrared spectrometer (Thermo Fisher Scientific, Waltham, MA, USA). Nonoxidized control and oxidized samples with different concentrations of FOs were diluted to 0.2 mg/mL protein concentration and scanned from 650 to 4000 nm. All spectra were averaged over three scans and corrected for the solvent signal. The data were processed by Fourier deconvolution to analyze the changes in the secondary structure of MPs.

Yu J., Wang S., Sun C, & Zhao B. (2023). Insights into Feruloylated Oligosaccharide Impact on Gel Properties of Oxidized Myofibrillar Proteins Based on the Changes in Their Spatial Structure. Foods, 12(6), 1222.

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Comprehensive Characterization of Lentinus Polysaccharides

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The contents of total polysaccharides, proteins, and uronic acids in LLP-D and LLP-W were analyzed by previously reported colorimetric methods [19 (link)]. The molecular weights and molecular weight distributions of LLPs were investigated by using high performance size exclusion chromatography equipped with the multi angle laser light scattering and the refractive index detector (Wyatt Technology Co., Santa Barbara, CA, USA) [20 (link)]. Besides, the apparent viscosities of LLPs were determined at the concentration of 10.0 mg/mL by using a Discovery Hybrid Rheometer-1 (TA instruments, New Castle, DE, USA) with a parallel steel plate (40.0 mm diameter and 1.0 mm gap) [20 (link)]. The constituent monosaccharides of LLPs were analyzed by using Thermo U3000 HPLC system (Thermo Fisher Scientific, Waltham, MA, USA) equipped with a Phenomenex gemini 5 μ C18 (150 mm × 4.6 mm) column followed by a formerly reported method [20 (link)]. Moreover, the FT-IR spectra of LLPs were recorded to analyze their chemical structures by using a Nicolet iS 10 FT-IR (ThermoFisher scientific, Waltham, MA, USA) [20 (link)]. Furthermore, the ¹H and ¹³C NMR spectra of LLPs were also recorded to further analyze their chemical structures by using a Bruker Ascend 600 MHz spectrometer with a z-gradient probe (Bruker, Rheinstetten, Germany) [20 (link)].

Wu D.T., Feng K.L., Huang L., Gan R.Y., Hu Y.C, & Zou L. (2021). Deep Eutectic Solvent-Assisted Extraction, Partially Structural Characterization, and Bioactivities of Acidic Polysaccharides from Lotus Leaves. Foods, 10(10), 2330.

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FT-IR Analysis of Larch Lentinan Polysaccharide

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The fourier transform infrared spectra (FT-IR) of LLP at different digested and fermented stages in vitro were recorded in the frequency range of 4000 - 500 cm⁻¹ by a Nicolet iS 10 FT-IR (Thermo Fisher Scientific, Waltham, MA, USA) (Wu et al., 2022 (link)). Additionally, the esterification degree (DE) values of LLP at different digested and fermented stages were also calculated through the FT-IR absorption bands around 1744 cm⁻¹ and 1611 cm⁻¹ by a previously reported method (Wu et al., 2022 (link)).

Wu D.T., Feng K.L., Li F., Hu Y.C., Wang S.P., Gan R.Y, & Zou L. (2022). In vitro digestive characteristics and microbial degradation of polysaccharides from lotus leaves and related effects on the modulation of intestinal microbiota. Current Research in Food Science, 5, 752-762.

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Characterization of Alq3 Morphology and Properties

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We investigated the morphology and particle size of both Alq₃ as rec and Alq₃ NP samples using a field-emission scanning electron microscope (FESEM), model JSM-7600F (JEOL, Japan). We recorded their X-ray powder diffraction (XRPD) patterns to study their crystallinity and crystal phases using an X-ray diffractometer, model Ultima IV (Rigaku, Japan). The XRPD patterns of the Alq₃ samples were scanned at room temperature and an angular range (2θ) of 5–28° in the step of 0.05° and a continuous mode (λ = 1.54 Å, I = 40 mA, and V = 40 kV). The chemical functional groups of Alq₃ were verified and characterized using a Fourier transform infrared (FTIR) spectrometer, model Nicolet iS10 FTIR (Thermo Scientific, USA). We recorded their FTIR spectra using the attenuated total reflection (ATR) sampling technique in which the ATR occurred in a germanium crystal. We recorded both photoluminescence (PL) excitation and emission spectra of the Alq₃ samples using a fluorescence spectrofluorophotometer, model RF-5301 PC (Shimadzu, Japan).

Saeed A., Madkhli A.Y., Pashameah R.A., Bataweel N.M., Razvi M.A, & Salah N. (2022). Antibacterial activity of the micro and nanostructures of the optical material tris(8-hydroxyquinoline)aluminum and its application as an antimicrobial coating. RSC Advances, 12(42), 27131-27144.

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Characterization of Peptide-Alginate Conjugation

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Peptide bonding to alginate was confirmed by FTIR and NMR. The KBr disc method was adopted in IR. Briefly, the lyophilized sample and KBr powder were mixed (approximately 1:150 in weight) and ground manually in an agate mortar with a pestle before the disc was pressed. The IR spectra were collected using a Nicolet^™ iS10 FT-IR (Thermo Fisher Scientific, USA) spectrometer at room temperature immediately after the preparation of the discs. Each spectrum was acquired by the accumulation of 36 scans at a resolution of 4 cm⁻¹.
¹H nuclear magnetic resonance (NMR) was performed on the dried sample to quantify complete GHK conjugation. High-resolution, ¹H NMR spectra were taken on a Avance DRX 800 (Bruker) spectrometer. Deuterated water (D₂O) was used as a solvent, and the sample concentrations were varied between 2.5 and 3 wt%. An accurately measured volume of the sample solution (550 μL) was transferred to a 5 mm NMR tube. ¹H NMR spectra were recorded at 800 MHz using a NMR spectrometer (Bruker Avance 600). Typically, 50 scans were collected into 32,000 data points over a spectral width of 0–16 ppm with a relaxation delay of 1 s and an acquisition time of 1.7 s. The spectra were phased corrected and integrated automatically using TOPSPIN.

Jose S., Hughbanks M.L., Binder B.Y., Ingavle G.C, & Leach J.K. (2014). Enhanced trophic factor secretion by mesenchymal stem/stromal cells with Glycine-Histidine-Lysine (GHK)-modified alginate hydrogels. Acta biomaterialia, 10(5), 1955-1964.

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Characterization of Polymer-Template Interactions

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A Shimadzu UV-3900 spectrophotometer (Shimadzu, Japan,), Nicolet IS 10 FT-IR (Thermo Fisher Scientific, Waltham, MA, USA), and AVANCE NEO 400 M ¹H NMR (Bruker, Switzerland) were used to examine interaction forces between the template molecules and functional monomers. The obtained polymer chemical structures were identified using a Nicolet IS 10 FT-IR spectrometer via the KBr compressed pellet method. The morphologies and structures of the polymer samples were determined using SEM (Zeiss Merlin Compact, Germany). Nitrogen adsorption tests were performed using an ASAP 2020 surface area and porosity analyzer (Micromeritics, Atlanta, GA, USA). Finally, biological sample analysis was performed using a Shimadzu Prominence LC-20A HPLC system (Shimadzu, Japan) equipped with a UV detector.

Song R., Xie J., Yu X., Ge J., Liu M, & Guo L. (2022). Preparation of Molecularly Imprinted Polymer Microspheres for Selective Solid-Phase Extraction of Capecitabine in Urine Samples. Polymers, 14(19), 3968.

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FT-IR Spectroscopy Analysis of Modified Polysaccharides

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The FT-IR spectra of WYP, WYP-S, WYP-G, WYP-I, WYPI-6, WYPI-12, WYPI-24, and WYPI-48 were recorded by a Nicolet iS 10 FT-IR (Thermo Fisher Scientific, Waltham, MA, USA). Besides, the esterification degree (DE) values were also calculated based on the FT-IR spectra as previously reported (Wu, Feng, et al., 2021 (link)).

Wu D.T., Liu W., Yuan Q., Gan R.Y., Hu Y.C., Wang S.P, & Zou L. (2022). Dynamic variations in physicochemical characteristics of oolong tea polysaccharides during simulated digestion and fecal fermentation in vitro. Food Chemistry: X, 14, 100288.

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Versatile Organic Synthesis Protocols

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All reagents used in the synthesis and functional assays were purchased from Sigma-Aldrich (St. Louis, MO) or Thermo Fisher Scientific (Waltham, MA) and utilized without additional purification. ¹H NMR and ¹³C NMR spectra were obtained on a Bruker 400 MHz spectrometer, and tetramethylsilane was used as an internal standard. Peak positions are given in parts per million (δ). Column chromatography was performed on silica gel (grade 60 mesh; Bodman Industries, Aston, PA). Routine thin-layer chromatography was performed on silica gel GHIF plates (250 μm, 2.5 × 10 cm; Analtech Inc., Newark, DE). MS spectra were obtained from a PerkinElmer Flexar UHPLC with an AxION 2 time-of-flight mass spectrometer, and the molecular weight of the compounds was within 0.005% of calculated values. Infrared spectra were obtained on Thermo Nicolet iS10 FT-IR. Purity of the compounds was determined by HPLC using Varian Microsorb 100-5 C18 column (250 × 4.6 mm), using Prostar 325 UV–vis (210 nm) as the detector. The HPLC parameters used were injection volume =15 μL, sample concentration = 3 mM, mobile phase = 60MeCN–40H₂O, flow rate = 1 mL/min.
The compounds were synthesized according to the general procedures for similar compounds.^{33 ,35 (link),39 (link)}

Pagare P.P., Ghatge M.S., Chen Q., Musayev F.N., Venitz J., Abdulmalik O., Zhang Y, & Safo M.K. (2020). Exploration of Structure–Activity Relationship of Aromatic Aldehydes Bearing Pyridinylmethoxy-Methyl Esters as Novel Antisickling Agents. Journal of medicinal chemistry, 63(23), 14724-14739.

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