Cyclosporin h

Manufactured by Merck Group

Sourced in United Kingdom

Cyclosporin H is a chemical compound used in laboratory research. It serves as a standard reference material for analytical procedures. The core function of Cyclosporin H is to provide a reliable and consistent substance for calibration, method development, and quality control purposes in various analytical applications.

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Lab products found in correlation

Lipofectamine rnaimax, by Thermo Fisher Scientific (2 mentions) Anti β actin, by Abcam (1 mentions) Torin1, by Bio-Techne (1 mentions) P70 s6 kinase, by Cell Signaling Technology (1 mentions) Cyclosporin a, by Bio-Techne (1 mentions) Phospho p70 s6 kinase thr389, by Cell Signaling Technology (1 mentions) Sqstm1 p62, by Cell Signaling Technology (1 mentions) Bafilomycin a1, by Bio-Techne (1 mentions) Bapta am, by Bio-Techne (1 mentions) Phospho mtor ser2448, by Cell Signaling Technology (1 mentions) Cycloheximide (chx), by Merck Group (1 mentions) N acetyl l cysteine, by Merck Group (1 mentions) L glutathione reduced, by Merck Group (1 mentions) Boc mlf, by Bio-Techne (1 mentions) Fpra14, by Bio-Techne (1 mentions) Ferrichrome, by Merck Group (1 mentions) Pyoverdine, by Merck Group (1 mentions) Deferiprone, by Merck Group (1 mentions) Chrome azurol s, by Thermo Fisher Scientific (1 mentions)

4 protocols using cyclosporin h

Squaramide Compounds Modulate Autophagy

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The squaramide compound SQ1, its analogue SQ2, and the control compound SQ3 (with no chloride transport activity) were previously described^{18 (link),19 (link)}. The following chemicals and antibodies were used in this study: Torin 1, bafilomycin A1, BAPTA AM, Cyclosporin A (Tocris Bioscience, Bristol, UK), Cycloheximide, Cyclosporin H, N-Acetyl-L-cysteine, L-Glutathione reduced (Sigma-Aldrich) oleate from Larodan (Solna, Sweden).
Anti-beta actin (Abcam 8226, Cambridge, UK), LC3 (#3868), mTOR (#2983), Phospho-mTOR (Ser2448) (#5536), LAMP1 (#9091), p70 S6 Kinase (#9202), Phospho-p70 S6 Kinase (Thr389) (#9205), SQSTM1/p62 (#7695) antibody were all purchased from Cell Signaling Technology, IF antibody Alexa Fluor® 647 anti-human CD107a (LAMP-1) Antibody from BioLegend.

Zhang S., Wang Y., Xie W., Howe E.N., Busschaert N., Sauvat A., Leduc M., Gomes-da-Silva L.C., Chen G., Martins I., Deng X., Maiuri L., Kepp O., Soussi T., Gale P.A., Zamzami N, & Kroemer G. (2019). Squaramide-based synthetic chloride transporters activate TFEB but block autophagic flux. Cell Death & Disease, 10(3), 242.

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Neuroblastoma Cell Lines Characterization

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Mouse and Human Neuroblastoma cells (N2a (ECACC 89121404), IMR-32 (ECACC 86041809), SH-SY5Y (ECACC 94030304)) were obtained from European Collection of Authenticated Cell Cultures (UK). Eagle’s Minimum Essential Medium high glucose (EMEM), Dulbecco’s Modified Eagle’s Medium high glucose (DMEM), all additional media components and cyclosporin H were purchased from Sigma Aldrich (UK). FPRa14, Boc-MLF and WRW4 were obtained from Tocris Bioscience (UK). Silencer^TM select siRNA duplexes for mouse Fpr1 (siRNA ID s66215), mouse Fpr2 (siRNA ID 66212) plus negative control no.1 siRNA and Lipofectamine RNAiMAX were purchased from ThermoFisher Scientific (UK). All other chemicals used were of reagent grade.

Cussell P.J., Howe M.S., Illingworth T.A., Gomez Escalada M., Milton N.G, & Paterson A.W. (2019). The formyl peptide receptor agonist FPRa14 induces differentiation of Neuro2a mouse neuroblastoma cells into multiple distinct morphologies which can be specifically inhibited with FPR antagonists and FPR knockdown using siRNA. PLoS ONE, 14(6), e0217815.

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Enterobactin and Siderophore Assays

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Enterobactin (Ent; from Escherichia coli) procured from Sigma-Aldrich (St. Louis, MO) is supplied free of iron and endotoxin (≥98% purity; HPLC). Iron-free Pyoverdine (from Pseudomonas fluorescens), ferrichrome (from Ustilago sphaerogena), Deferiprone, deferoxamine mesylate salt (DFO; from Streptomyces pilosus), 2,3 dihydroxybenzoic acid (2,3-DHBA), lipopolysaccharide (LPS) from E. coli 0128:B12, dimethyl sulfoxide (DMSO), ferric chloride (FeCl3, Fe³⁺), 2,2ʹ-dipyridyl, and cyclosporin H were purchased from Sigma-Aldrich (St. Louis, MO). Flagellin (FliC) from Salmonella enterica subsp. enterica serovar Typhimurium (SL3201, ﬂjB-), a kind gift from Dr. Andrew Gewirtz, Georgia State University, was puriﬁed through sequential cation and anion-exchange chromatography as previously described.^{52 (link)} FITC Annexin-V apoptosis detection kit was purchased from Molecular Probes (Life Technologies, Columbus, OH). Chrome azurol S (CAS) was purchased from Acros Organics (Geel, Belgium). Recombinant mouse Lcn2 (alias neutrophil gelatinase-associated lipocalin) (rec-Lcn2; Cat# CM17) produced by a mammalian (human) expression system was procured from Novoprotein Scientific Inc. (Fremont, CA). Carrier-free rec-Lcn2 was supplied at a purity ≥95% as determined by reducing SDS-PAGE and free from endotoxin, siderophore, and iron.

Saha P., Yeoh B.S., Xiao X., Golonka R.M., Abokor A.A., Wenceslau C.F., Shah Y.M., Joe B, & Vijay-Kumar M. (2020). Enterobactin induces the chemokine, interleukin-8, from intestinal epithelia by chelating intracellular iron. Gut Microbes, 12(1), 1841548.

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Ex vivo erythropoiesis from CD34+ HSPCs

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Purified CD34⁺ HSPCs were seeded in HEM supplemented with 2 µM cyclosporin H (Sigma-Aldrich). After 24 h, CD34⁺ cells were transduced with Ery-Lin-shRNA LVs at a multiplicity of infection (MOI) of ~ 10 by spinfection and incubated for 24 h. The medium then was changed, and the cells were used for ex vivo erythropoiesis or mouse transplantation experiments.

Bagchi A., Devaraju N., Chambayil K., Rajendiran V., Venkatesan V., Sayed N., Pai A.A., Nath A., David E., Nakamura Y., Balasubramanian P., Srivastava A., Thangavel S., Mohankumar K.M, & Velayudhan S.R. (2022). Erythroid lineage-specific lentiviral RNAi vectors suitable for molecular functional studies and therapeutic applications. Scientific Reports, 12, 14033.

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