0.5 μg of RNeasy-purified total RNA (QIAGEN 74104) was retro-transcribed with the High-Capacity cDNA Reverse Transcription Kit (Thermo 4368813) by following the manufacturer’s instructions. The real-time PCR amplification was performed with SYBR Green Supermix (Bio-Rad 172–5124) in a Bio-Rad CFX96TM Real-Time machine. The PCR program was 3 min at 95°C and 40 cycles of (15 s at 95°C and 30 s at 60°C). Relative RNA quantification relied on the ΔΔCT method.
Rneasy purified total rna
Manufactured by Qiagen
The RNeasy-purified total RNA is a laboratory equipment product that is used for the purification of total RNA from various biological samples. It provides a fast and efficient method for the isolation of high-quality RNA, which can be used for a variety of downstream applications such as gene expression analysis, RT-PCR, and microarray studies.
Automatically generated - may contain errors
2 protocols using rneasy purified total rna
1
Quantitative RNA Expression Analysis
2
Quantitative Real-Time PCR Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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In all, 0.5 μg of RNeasy-purified total RNA (Qiagen 74104) was retro-transcribed with the High-Capacity cDNA Reverse Transcription Kit (Thermo 4368813) by following the manufacturer’s instructions. The real-time PCR amplification was performed with SYBR Green Supermix (Bio-Rad 172-5124) in a Bio-Rad CFX96TM Real-Time machine. The PCR program was 3 min at 95 °C and 40 cycles of (15 s at 95 °C and 30 s at 60 °C). Relative RNA quantification relied on the ∆∆CT method. Primer sequences can be found in Supplementary Data 1 .
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