Cells were exposed to DMEM containing 80 μCi/mL of L-[S35]-Methionine (Hartmann Analytics, #KSM-01) and 100 µg/mL emetine (Sigma-Aldrich #E2375) for 1 h at 37 °C. Myotubes were washed, lysed, and resolved in SDS-PAGE gels. Gels were fixated, stained with Coomassie Blue, dried, and exposed to a phosphorscreen for 3 d. Images were obtained using a Typhoon™ FLA 7000 phosphorimager and quantified by measuring average grey values in ImageJ v1.52a.
E2375
Manufactured by Merck Group
The E2375 is a laboratory equipment product from Merck Group. It is a general-purpose device used for various applications in scientific research and testing. The core function of the E2375 is to perform precise measurements and analyses. Further details about its intended use or specific applications are not available.
Automatically generated - may contain errors
3 protocols using e2375
1
Pulse-chase analysis of protein synthesis
2
Sourcing and Handling Chemical Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The following compounds were obtained from Sigma: CHX (100 mg/mL stock in DMSO; C1988), PURO (10 mg/mL stock in water; P9620), ANS (10 mg/mL stock in DMSO; A5862), EME (100 mg/mL stock in water; E2375), and HHT (10 mg/mL stock in DMSO; SML1091). LTM (5 mM stock in DMSO; 506291) was obtained from Millipore. BVD (10 mM stock in DMSO) and DIDB (10 mM in DMSO) were kind gifts from Tin Tin Su (University of Colorado at Boulder) and Malia Potts (St. Jude Children's Research Hospital), respectively. NSC119893 (50 mM stock in DMSO) was obtained from the National Cancer Institute Development Therapeutics Program.
3
Emetine Treatment of Sea Urchin Embryos
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For emetine treatments, a stock solution of 100 mM emetine dihydrochloride hydrate (Sigma-Aldrich E2375) was prepared in deionized water and stored at −20 °C. Immediately before use, the stock solution was diluted 1:1,000 in ASW (final [emetine] = 100 μM). After embryos were loaded into a microsurgical chamber, the fluid in the chamber was replaced with 100 μM emetine, and microsurgery was carried out in the presence of the inhibitor. PMC(−) embryos were allowed to develop for an additional 2 hours in emetine-containing seawater before they were processed for WMISH.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!