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2 protocols using β actin antibody af7018

1

Comprehensive Renal and Oxidative Stress Assays

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The Urine protein test kit (C035-2-1), Urea Assay kit (C013-2-1), Creatinin Assay kit (C011-2-1), Malondialdehyde (MDA) assay kit (A003-1-2), Reduced glutathione (GSH) assay kit (A006-2-1), tissue iron assay kit (A039-2-1) were all obtained from Jiancheng Bioengineering Institute (Nanjing, China). The Dihydroethidium (S0063) was obtained from Beyotime (Jiangsu, China). Streptozotocin (STZ) (S0130-1g) was acquired from Sigma-Aldrich (St. Louis, Missouri, USA). Ferrous Ion Content Assay Kit (BC5415) was acquired from Solarbio (Beijing, China). qPCR SYBR Green Master Mix (11198ES08) was purchased from Yeasen Biotechnology (Shanghai, China). GPX4 antibody(67763-1-Ig), GAPDH antibody (60004-1-Ig) and α-SMA antibody (67735-1-Ig) were purchased from Proteintech (Wuhan, China). HIF-1α antibody (340462), HO-1 antibody (R24541) and Fibronectin antibody (250073) were purchased from ZEN-BIOSCIENCE (Chengdu, China). Vimentin antibody (AF7013) and β-actin antibody (AF7018) were purchased from Affinity Biosciences (Jiangsu, China). HRP AffiniPure Goat Anti-Mouse IgG (H+L) (FDM007) and HRP AffiniPure Goat Anti-Rabbit IgG (H+L) (FDR007) were obtained from Fude Biological Technology (Hangzhou, China).
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2

Mitochondrial Regulation in Inflammation

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The AMPK activator AICAR (S1802), NLRP3 inhibitor MCC950 (S8930), and Drp1 inhibitor Mdivi-1 (S7162) were purchased from Selleck Chemicals (Houston, USA). Anti-GFAP antibody (ab4648) was purchased from Abcam (Cambridge, UK); NLRP3 rabbit pAb (A12694), Caspase-1 rabbit pAb (A0964), IL-1β rabbit pAb (A1112), NLRP1 rabbit pAb (A16212) and NLRC4 rabbit pAb (A7382) were purchased from ABclonal Technology (Wuhan, China); AMPK alpha antibody (AF6423), phospho-AMPK alpha (Thr172) antibody (AF3423), Drp1 antibody (DF7073), phospho-Drp1 (Ser637) antibody (DF2980), phospho-Drp1 (Ser616) antibody (AF8470), AIM2 antibody (DF3514) and β-actin antibody (AF7018) were purchased from Affinity Biosciences (Changzhou, China). A mitochondrial membrane potential assay kit with JC-1 (C2006) was purchased from Beyotime (Shanghai, China). MitoSOX
TM Red mitochondrial superoxide indicator was purchased from Thermo Fisher Scientific (Waltham, USA). RPMI 1640 medium, Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), Pen Strep (penicillin-streptomycin), and trypsin were obtained from Gibco (Carlsbad, USA).
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