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Realplex real time pcr instrument

Manufactured by Eppendorf
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The Realplex Eppendorf Real-Time PCR instrument is a laboratory equipment designed for quantitative real-time polymerase chain reaction (qRT-PCR) analysis. It is capable of detecting and measuring the amplification of DNA or RNA sequences in real-time during the PCR process.

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Lab products found in correlation

Realplex sequence detection system, by Eppendorf (1 mentions) Applied biosystems stepone plus real time pcr systems, by Thermo Fisher Scientific (1 mentions) Rneasy mini kit, by Qiagen (1 mentions)

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Realplex Instrument

2 protocols using realplex real time pcr instrument

1

Quantification of Stat3 Expression

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Total RNA was obtained by phenol/chloroform separation. Gapdh (forward: 5′-GACATGCCGCCTGGAGAAAC-3′; reverse 5′-AGCCCAGGATGCCCTTTAGT-3′) was used as the control house-keeping gene. The primers for Stat3fl/fl detection were: forward 5′-GGGGTGAGAGTTACCGTGAA-3′ and reverse 5′-CACACACACACAAGCCATCA-3′. Real-time PCR was performed with a SYBR green PCR mix (ABI biosystems, Waltham, MA) in a Realplex Eppendorf Real-Time PCR instrument (Eppendorf, Hamburg, Germany).
Nieves E.C., Toubai T., Peltier D.C., Oravecz-Wilson K., Liu C., Tamaki H., Sun Y, & Reddy P. (2017). STAT3 expression in host myeloid cells controls GVHD severity. Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation, 23(10), 1622-1630.
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2

Quantitative Real-Time RT-PCR Analysis

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RNA was isolated with an RNeasy Mini kit (Qiagen) and cDNA was generated by reverse transcription (Applied Biosystems). Real-time RT-PCR was performed with a SYBR green PCR mix (ABI Biosystems) in the Realplex Eppendorf Real-time PCR instrument (Eppendorf AG). Gene expression levels were calculated relative to the 18s gene. Data were collected and quantitatively analyzed on a Realplex sequence detection system (Eppendorf AG), and Applied Biosystems StepOne Plus Real-time PCR systems (Applied Biosystems). The primer sequences are listed in Supplementary Table 1.
He S., Liu Y., Meng L., Sun H., Wang Y., Ji Y., Purushe J., Chen P., Li C., Madzo J., Issa J.P., Soboloff J., Reshef R., Moore B., Gattinoni L, & Zhang Y. (2017). Ezh2 phosphorylation state determines its capacity to maintain CD8+ T memory precursors for antitumor immunity. Nature Communications, 8, 2125.
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