Human and mouse ifn γ elisa kits

Manufactured by BioLegend

The Human and Mouse IFN-γ ELISA Kits are quantitative sandwich enzyme-linked immunosorbent assay (ELISA) kits designed for the measurement of interferon-gamma (IFN-γ) levels in human and mouse biological samples.

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Lab products found in correlation

Magpix, by Merck Group (1 mentions) Xponent 4, by Merck Group (1 mentions)

Close spelling variants of this product

Human IFN-γ ELISA kits Mouse IFN-γ ELISA kit IFN-γ ELISA kit Mouse IFN-γ ELISA IFN-γ ELISA Human IFN-γ Mouse IFN-γ Mouse ELISA kits IFN-γ ELISAs

2 protocols using human and mouse ifn γ elisa kits

Cytokine Secretion Assay Protocol

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1 × 10⁴ target cells were cocultured with effector cells at an effector cell: target cell (E: T) ratio of 10: 1 in round-bottom 96-well culture plates for 4 h. Cell-free supernatants were assayed for cytokine secretion by enzyme-linked immunosorbent assay (ELISA) kits according to the manufacturer’s protocol. Human and mouse IFN-γ ELISA kits were purchased from BioLegend.

Kim K.S., Choi B., Choi H., Ko M.J., Kim D.H, & Kim D.H. (2022). Enhanced natural killer cell anti-tumor activity with nanoparticles mediated ferroptosis and potential therapeutic application in prostate cancer. Journal of Nanobiotechnology, 20, 428.

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Multiplex ELISA for IFN-γ Detection

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Human and mouse IFN-γ ELISA kits were purchased from Biolegend (San Diego, CA) and assay was performed as described by manufacturer. The plates were read in a microplate reader at 450 nm to obtain absorbance value. Multiplex assay was conducted as described in the manufacturer’s protocol for each specified kit. Analysis was performed using a Luminex multiplex instrument (MAGPIX, Millipore, Billerica, MA), and data was analyzed using the proprietary software (xPONENT 4.2, Millipore, Billerica, MA).

Kaur K., Sun Y., Kanayama K., Morinaga K., Hokugo A., Nishimura I, & Jewett A. (2023). Augmentation of IFN-γ by bone marrow derived immune cells in the presence of severe suppression of IFN-γ in gingivae induced by zoledronic acid and denosumab in Hu-BLT mice model of ONJ. Frontiers in Endocrinology, 14, 1111627.

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