The largest database of trusted experimental protocols

Cpt1a ab128568

Manufactured by Abcam

The CPT1A (ab128568) is a rabbit monoclonal antibody that recognizes the Carnitine Palmitoyltransferase 1A (CPT1A) protein. CPT1A is a key enzyme in the mitochondrial beta-oxidation of long-chain fatty acids. This antibody can be used for the detection of CPT1A in various applications.

Automatically generated - may contain errors

2 protocols using cpt1a ab128568

1

PEITC Metabolism and Lipogenesis

Check if the same lab product or an alternative is used in the 5 most similar protocols
PEITC (purity ≥98%) was purchased from LKT Laboratories (St. Paul, MN). RPMI1640 medium was from Cellgro-Mediatech (Manassas, VA), whereas other cell culture reagents, fetal bovine serum, and BODIPY® 493/503 (D3922) were purchased from Life Technologies-Thermo Fisher Scientific (Waltham MA). Anti-FASN (3180) and anti-ACC1 (4190) antibodies were from Cell Signaling Technology (Danvers, MA), whereas anti-ACLY (ab40793) and anti-carnitine palmitoyltransferase 1A (CPT1A) (ab128568) antibodies were from Abcam (Cambridge, MA). A kit for colorimetric measurement of triglyceride (10010303) was from Cayman Chemical (Ann Arbor, MI). Total phospholipid (MAK122) and total free fatty acid (MAK044) quantification kits were from Sigma-Aldrich (St. Louis, MO). A fluorometric kit for acetyl-CoA (K317-100) and a colorimetric kit for ATP (K354-100) were purchased from BioVision (Milpitas, CA).
+ Open protocol
+ Expand
2

Transcriptional Regulation by ETV5, PPARα, and PPARγ

Check if the same lab product or an alternative is used in the 5 most similar protocols
For the antibodies, ETV5 (PA5-30023) was obtained from Thermo Fisher Scientific. PPARa (ab24509) and CPT1A (ab128568) antibodies were from Abcam. S6 (2313), phosphorylated S6 (pS6) (4858), mTOR (2972), phosphorylated mTOR (pmTOR) (2971), myc (9B11), flag (D6W5B), and ATGL (2439) antibodies were from Cell Signaling Technology. ETV5 (13011-1-AP), actin (60008-1-Ig), b-tubulin (10094-1-AP), and GAPDH (60004-1-Ig) antibodies were from Proteintech. For the plasmids, pCMV-ETV5-mycflag, pCMV-PPARa-myc-flag, and pCMV-PPARg-myc-flag were obtained from Origene. Luciferase vectors pGL4.20 and pGL4.74 were obtained from Promega.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!