Purelab ultra system

Manufactured by Elga LabWater

Sourced in Germany, United Kingdom

The Purelab Ultra system is a water purification device designed to produce ultrapure water for laboratory applications. It utilizes a combination of filtration and deionization techniques to remove impurities and deliver water of high purity.

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Lab products found in correlation

Acetonitrile, by Merck Group (7 mentions) Ammonium acetate, by Merck Group (5 mentions) Ammonium acetate ms grade, by Merck Group (4 mentions) Glacial acetic acid p a, by Merck Group (3 mentions) Methanol, by Merck Group (3 mentions) Methanol lc gradient grade, by Merck Group (3 mentions) Sucrose, by Biolife (2 mentions) Yeast extract, by Biolife (2 mentions) Potato dextrose agar (pda), by Biolife (2 mentions) Glacial acetic acid, by Merck Group (2 mentions) Propylene glycol, by Merck Group (2 mentions) Formic acid, by Thermo Fisher Scientific (2 mentions) K2co3, by Merck Group (2 mentions) α zel, by Romer Labs (1 mentions) β zel, by Romer Labs (1 mentions) Methanol meoh, by Avantor (1 mentions) Acetonitrile acn, by Avantor (1 mentions) Ethanol, by Merck Group (1 mentions) Acetonitrile, by Avantor (1 mentions) Methanol, by Avantor (1 mentions)

Spelling variants of this product

PURELAB Ultra (14 citations) Purelab Ultra water purification system (4 citations) PureLab Ultra Analytic System (4 citations) PureLab Ultra Water System (2 citations)

20 protocols using purelab ultra system

Zearalenone and metabolite analysis

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M ethanol (MeOH) and acetonitrile (ACN) (both LC grade) were purchased from VWR International GmbH (Vienna, Austria). Glacial acetic acid (LC-MS grade) and ethanol were obtained from Sigma-Aldrich (Vienna, Austria) and from Carl Roth GmbH and Co. KG (Karlsruhe, Germany), respectively. Water was purified with a Purelab Ultra system (ELGA LabWater, Celle, Germany).
For method validation, reference standard solutions of ZEN (100.8 µg/mL in ACN), α-ZEL (10.4 µg/mL in ACN), and β-ZEL (9.9 µg/mL in ACN) from Romer Labs (Tulln, Austria) were used. Solid ZEN-14-S was also obtained from Romer Labs. ZEN was previously purified from 24-day-old rice cultures inoculated with Fusarium graminearum (NRRL 66037) as described in Michlmayr et al. [42 ]. ZEN-glucosides were obtained by enzymatic conversion of ZEN by a barley UDP-glucosyltransferase (HvUGT14077) and purified by preparative HPLC [42 ]. Lyophilized compounds were dissolved in ethanol for use as analytical standards and the stock solutions containing between 991 (ZEN-14-S) and 1274 (ZEN-14-Glc) µg/mL of the analytes were stored at −20 °C. For spiking experiments further dilutions were prepared in MeOH/water (20/80, v/v). ZEN- and ZEL-glucuronides were synthesized as described in Mikula et al. [34 (link),43 (link)].

Binder S.B., Schwartz-Zimmermann H.E., Varga E., Bichl G., Michlmayr H., Adam G, & Berthiller F. (2017). Metabolism of Zearalenone and Its Major Modified Forms in Pigs. Toxins, 9(2), 56.

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Fungal Metabolites Quantification

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Acetonitrile, methanol and glacial acetic were purchased from VWR Chemicals (Vienna, Austria). Ammonium acetate was acquired from Sigma-Aldrich (Vienna, Austria). A Purelab Ultra system (ELGA LabWater, Celle, Germany) was used for further purification of reverse osmosis water. Standards of fungal metabolites were purchased from several sources as described in [31 (link)].

Izzo L., Mikušová P., Lombardi S., Sulyok M, & Ritieni A. (2022). Analysis of Mycotoxin and Secondary Metabolites in Commercial and Traditional Slovak Cheese Samples. Toxins, 14(2), 134.

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Extraction and Characterization of ε-Viniferin

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ε-Viniferin (purity ≥ 95%) was extracted and purified from grapevine shoot extract in our laboratory as described previously [21 (link)]. The chemical structure was confirmed by 1H NMR, and high-resolution mass spectrometry [22 ]. High pressure liquid chromatography (HPLC)-grade acetonitrile, methanol, ethanol, ethyl acetate and formic acid were purchased from Fisher Scientific (Illkirch, France). Ultrapure water used was obtained using a Purelab Ultra System (Elga Lab Water, High Wycombe, UK).

Courtois A., Atgié C., Marchal A., Hornedo-Ortega R., Lapèze C., Faure C., Richard T, & Krisa S. (2018). Tissular Distribution and Metabolism of trans-ε-Viniferin after Intraperitoneal Injection in Rat. Nutrients, 10(11), 1660.

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Phytotoxicity Evaluation of Mellein

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Sucrose, sodium nitrate, potassium chloride, dipotassium phosphate, magnesium sulfate, iron (II) sulfate, and agar were purchased from Fisher Chemicals (Fair Lawn, NJ, USA). Enantiomerically pure (R)-(-)-mellein purchased from Cayman Chemical (Ann Arbor, MI, USA) with a listed purity of ≥98%, which was confirmed by GC-MS analysis (Chen, 2017 (link)), was used as an LC-MS/MS standard and for phytotoxicity testing with soybean seedlings. 3-Nitropropionic acid for use as an LC-MS/MS standard was purchased from Sigma-Aldrich (Vienna, Austria). For culture medium preparation and phytotoxicity assays, reverse osmosis water was purified by a Barnstead E-Pure Ultrapure Water Purification System (Thermo Scientific, Marietta, OH, USA).
Reagents for LC-MS/MS analysis consisted of LC-MS grade ammonium acetate and glacial acetic acid purchased from Sigma-Aldrich (Vienna, Austria), LC-MS Chromasolv grade methanol purchased from Honeywell (Seelze, Germany), HiPerSolv Chromanorm HPLC gradient grade acetonitrile purchased from VWR Chemicals (Vienna, Austria), and reverse osmosis water purified using a Purelab Ultra system (ELGA LabWater, Celle, Germany).

Khambhati V.H., Abbas H.K., Sulyok M., Tomaso-Peterson M., Chen J, & Shier W.T. (2023). Mellein: Production in culture by Macrophomina phaseolina isolates from soybean plants exhibiting symptoms of charcoal rot and its role in pathology. Frontiers in Plant Science, 14, 1105590.

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Quantification of Levonorgestrel in Human Plasma

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LNG (MW: 312.45, 98% pure) was purchased from Sigma, UK. The internal standard D-(−)-norgestrel-d7 (MW: 319.49, 98% pure) was purchased from Toronto Research Chemicals (North York, Ontario, Canada). The chemical structures of LNG and IS are shown in Figure 1. Acetonitrile (LC/MS grade), methanol (HPLC grade), and tert-Butyl methyl ether (reagent grade >98% pure) were purchased from Sigma, UK. Ammonia in aqueous solution (25% AnalaR NORMAPUR®) was purchased from VWR International (Lutterworth, UK). HPLC grade de-ionized water was produced from an Elga Option S water purifier (Elga LabWater, High Wycombe, UK) and was further purified to 18.2 MΩ with a Purelab Ultra system (Elga LabWater, High Wycombe, UK). Blank human plasma (K₂EDTA) was obtained from the National blood service (Liverpool, UK).

Cirrincione L.R., Penchala S.D., Scarsi K.K., Podany A.T., Winchester L.C., Back D.J., Khoo S.H., Fletcher C.V., Siccardi M, & Else L.J. (2018). Development, validation and utilization of a highly sensitive LC-MS/MS method for quantification of levonorgestrel released from a subdermal implant in human plasma. Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 1084, 106-112.

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Volatile Profiling of Spirulina Products

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The analyzed samples (17 in total) included sixteen commercial spirulina products from various producers and geographical origins, collected from stores in Athens, Greece and one raw biomass sample (Table 1, sample S2), obtained from a local Greek producer. Analytical standards of 4-(2,6,6-trimethyl-1-cyclohexen-1-yl)-3-buten-2-one (β-ionone), pentan-1-ol, hexanal, hexan-1-ol, heptan-2-one, benzaldehyde, butan-1-ol, 1,3,3-trimethyl-2-oxabicyclo[2.2.2]octane (eucalyptol), 2,4-di-tert-butylphenol, butan-2-one, naphthalene, 1-isopropyl-4-methyl-1,4-cyclohexadiene (γ-terpinene), heptan-1-ol, oct-1-en-3-ol, 2,6,6-trimethyl-1-cyclohexene-1-carboxaldehyde (β-cyclocitral), octan-1-ol, tetradecane, 4-(2,6,6-trimethylcyclohex-2-en-1-yl)but-3-en-2-one (α-ionone), pentadecane, pent-1-en-3-one, hexadecane, and heptadecane, were obtained from Sigma-Aldrich; Merck KGaA (Darmstadt, Germany). A standard mixture of these twenty-two compounds was prepared at a concentration of 10 μg/L in ultrapure water. A standard mixture of alkanes (C7–C30) from Supelco; Merck KGaA (Darmstadt, Germany) was used for retention index calibration. Ultrapure water (18.2 MΩ) was obtained from a Purelab Ultra system (Elga - LabWater, High Wycombe, UK).

Paraskevopoulou A., Kaloudis T., Hiskia A., Steinhaus M., Dimotikali D, & Triantis T.M. (2024). Volatile Profiling of Spirulina Food Supplements. Foods, 13(8), 1257.

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Bronchodilator Preparation and Characterization

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FOR, IND, and TIO were obtained from CHEMO Industriale Chimica s.r.l. (Saronno (VA), Italy). Methacholine was purchased from Sigma-Aldrich (St. Louis, MO, USA), and atropine sulfate from Merck (Darmstadt, Germany). Dimethyl sulfoxide was purchased from Carl Roth GmbH + Co., KG (Karlsruhe, Germany). All components used for the preparation of the Krebs-Henseleit solution (i.e., KCl, NaCl, NaHCO₃, MgSO₄, KH₂SO₄, CaCl₂, and glucose) were purchased from VWR (Radnor, Pennsylvania, USA). Ultrapure water was obtained from a Purelab-Ultra system (Elga LabWater, Lane End, UK).
Solutions of bronchodilators were prepared daily using Dimethyl sulfoxide for the stock solution and were diluted in ultrapure water. Solutions of Methacholine and atropine sulfate were prepared in ultrapure water.

Menchi E., El Khattabi C., Pochet S., Denis O., Amighi K, & Wauthoz N. (2022). Optimization of Long-Acting Bronchodilator Dose Ratios Using Isolated Guinea Pig Tracheal Rings for Synergistic Combination Therapy in Asthma and COPD. Pharmaceuticals, 15(8), 963.

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Ultrapure Water Production and Reagent Preparation

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Type I Ultrapure Water was produced by an ELGA Purelab Ultra system (Elga LabWater, High Wycombe, United Kingdom) and used to create solutions throughout. Ammonium bicarbonate, potassium chloride, formic acid, tolylsulfonyl phenylalanyl chloromethyl ketone-treated trypsin from bovine pancreas was obtained from Sigma-Aldrich (Steinheim, Germany). Trifluoroacetic acid, disodium hydrogen phosphate dihydrate, potassium dihydrogen phosphate, and sodium chloride were purchased from Merck (Darmstadt, Germany). HPLC-supra-gradient acetonitrile was obtained from Biosolve (Valkenswaard, The Netherlands). The Visucon-F pooled healthy human plasma standard originated from Affinity Biologicals (Ancaster, Canada). Protein G Sepharose 4 Fast Flow beads were obtained from GE Healthcare (Uppsala, Sweden). Recombinant trimerized S protein was prepared as described.¹⁵ (link)

Pongracz T., Nouta J., Wang W., van Meijgaarden K.E., Linty F., Vidarsson G., Joosten S.A., Ottenhoff T.H., Hokke C.H., de Vries J.J., Arbous S.M., Roukens A.H, & Wuhrer M. (2022). Immunoglobulin G1 Fc glycosylation as an early hallmark of severe COVID-19. EBioMedicine, 78, 103957.

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HPLC-Based Aflatoxin Detection Protocol

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Yeast extract, potato dextrose agar and sucrose were purchased from Biolife (Italy) while aflatoxin standard mix (B₁, G₁, B₂, G₂) was purchased from Biopure (Austria). Acetonitrile and methanol (both HPLC grade) were obtained from Merck (Germany). Ammonium acetate and glacial acetic acid (p.a.) were purchased from Sigma Aldrich (Vienna, Austria). A Purelab Ultra system (ELGA LabWater, Celle, Germany) was used for ultrapure water preparation. Standards of A. flavus metabolites were purchased from Various research groups or from the following commercial sources: Romer Labs®Inc.(Tulln, Austria), Sigma–Aldrich (Vienna, Austria), Iris Biotech GmbH (Marktredwitz, Germany), Axxora Europe (Lausanne, Switzerland) And LGC Promochem GmbH (Wesel, Germany) and prepared as described by Malachova et al.^{46 (link)}.

Kovač T., Borišev I., Crevar B., Čačić Kenjerić F., Kovač M., Strelec I., Ezekiel C.N., Sulyok M., Krska R, & Šarkanj B. (2018). Fullerol C60(OH)24 nanoparticles modulate aflatoxin B1 biosynthesis in Aspergillus flavus. Scientific Reports, 8, 12855.

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Preparation of Analytical Standards

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LC gradient-grade
methanol and acetonitrile and MS-grade ammonium acetate and glacial
acetic acid (p.a.) were purchased from Sigma-Aldrich (Vienna, Austria).
A Purelab Ultra system (ELGA Lab Water, Celle, Germany) was used for
further purification of reverse osmosis water.
Standards of
fungal and bacterial metabolites, pesticides, and pharmaceuticals
were either purchased from Romer Labs Inc. (Tulln, Austria), Sigma-Aldrich
(Vienna, Austria), Iris Biotech GmbH (Marktredwitz, Germany), Axxora
Europe (Lausanne, Switzerland), NEOCHEMA GmbH (Bodenheim, Germany),
Restek GmbH (Bad Homburg, Germany), BioAustralis (Smithfield, Australia),
AnalytiCon Discovery (Potsdam, Germany), Adipogen AG (Liestal, Switzerland),
and LGC Promochem GmbH (Wesel, Germany) or were obtained as gifts
from various research groups. Each analyte was dissolved in acetonitrile
(primarily), acetonitrile/water 1:1 (v/v), methanol, methanol/water
1:1 (v/v), or water.
By mixing the stock solutions of the corresponding analyte, 74
combined working solutions were prepared for fungal toxins, 9 working
solutions for pesticides, and 8 for pharmaceutical active agents and
were stored at −20 °C. For spiking purposes, a liquid
multi-analyte standard was freshly prepared by combining the intermediate
working mixtures deriving from liquid stock solutions.

Steiner D., Krska R., Malachová A., Taschl I, & Sulyok M. (2020). Evaluation of Matrix Effects and Extraction Efficiencies of LC–MS/MS Methods as the Essential Part for Proper Validation of Multiclass Contaminants in Complex Feed. Journal of Agricultural and Food Chemistry, 68(12), 3868-3880.

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