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Racked collection microtubes

Manufactured by Qiagen
Sourced in Germany

Racked collection microtubes are a type of laboratory equipment used for sample collection and storage. They provide a convenient and organized way to handle and transport multiple small samples or aliquots. The key function of these microtubes is to securely hold and store samples in a standardized format.

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3 protocols using racked collection microtubes

1

DNA Extraction from Barley Samples

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Genomic DNA was extracted from the basal leaf segments of the youngest fully developed leaves of barley plants (BBCH 10-53), roots, leaf blade-like structures, and grains' leftovers (without husks) of the seedlings removed from the soil, and grains (without husks) removed from the soil and embryos and/or endosperms with most of the intact bran (remaining after the embryo extirpation) of the mature imbibed grains using the cetyltrimethylammonium bromide (CTAB). Husks were removed manually from the grains and grain leftovers before the DNA extarction procedure. DNA extraction was performed by using either 2-ml Eppendorf tubes or 1.2-ml racked collection microtubes (Qiagen, Hilden, Germany) depending on the number and type of samples processed.
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2

DNA Extraction Protocol for Caraway

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A protocol for DNA extraction in barley (Brandes, in prep.) was slightly modified for DNA extraction in caraway. 192 plants per each F 1 population (n = 7) were grown in greenhouse in 96 pot plates. The youngest developing leaf per plant (BBCH = 15-18 (Hack et al. 1992 )) was sampled. Leaves were collected in racked collection microtubes (Cat. no. 19560, Qiagen) . Two 4 mm steel beads per sample were added. Samples were frozen with liquid nitrogen. Samples were ground using mixer mill MM 300 (Retsch). Samples were incubated at 65 °C for 45 min after adding 600 ll extraction buffer (1 M guanidine thiocyanate, 2 M NaCl, 30 mM NaAc) per sample. Samples were spun using Rotina 420 centrifuge (Hettich) for 30 min at 4000 rpm. 400 ll of liquid per sample were transferred to new collection microtubes and 2.5 ll RNase A (5 mg/ml) was added. Samples were incubated for 30 min at 35 °C. DNA was precipitated, adding 300 ll propan-2-ol per sample. Samples were spun using Rotina 420 centrifuge (Hettich) for 30 min at 4000 rpm. DNA pellets were washed in two steps, adding 600 ll per solution and sample (wash solution 1: 76% ethanol, 200 mM NaAc; wash solution 2: 76% ethanol, 10 mM NaAc). Dried DNA was dissolved in 600 ll TE-buffer (10 mM Tris/HCl, 1 mM EDTA) per sample. DNA was quantified using nanodrop 8000 spectrophometer (Thermo Fisher Scientific).
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3

DNA Extraction Protocol for Caraway

Check if the same lab product or an alternative is used in the 5 most similar protocols
A protocol for DNA extraction in barley (Brandes, in prep.) was slightly modified for DNA extraction in caraway. 192 plants per each F 1 population (n = 7) were grown in greenhouse in 96 pot plates. The youngest developing leaf per plant (BBCH = 15-18 (Hack et al. 1992 )) was sampled. Leaves were collected in racked collection microtubes (Cat. no. 19560, Qiagen) . Two 4 mm steel beads per sample were added. Samples were frozen with liquid nitrogen. Samples were ground using mixer mill MM 300 (Retsch). Samples were incubated at 65 °C for 45 min after adding 600 ll extraction buffer (1 M guanidine thiocyanate, 2 M NaCl, 30 mM NaAc) per sample. Samples were spun using Rotina 420 centrifuge (Hettich) for 30 min at 4000 rpm. 400 ll of liquid per sample were transferred to new collection microtubes and 2.5 ll RNase A (5 mg/ml) was added. Samples were incubated for 30 min at 35 °C. DNA was precipitated, adding 300 ll propan-2-ol per sample. Samples were spun using Rotina 420 centrifuge (Hettich) for 30 min at 4000 rpm. DNA pellets were washed in two steps, adding 600 ll per solution and sample (wash solution 1: 76% ethanol, 200 mM NaAc; wash solution 2: 76% ethanol, 10 mM NaAc). Dried DNA was dissolved in 600 ll TE-buffer (10 mM Tris/HCl, 1 mM EDTA) per sample. DNA was quantified using nanodrop 8000 spectrophometer (Thermo Fisher Scientific).
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