PBMCs were isolated from whole blood collected from family members and healthy donors by ficoll-histopaque gradient centrifugation. For phenotypic staining, the following monoclonal antibodies were used: CD3-APC-H7, CD4-PerCP-Cy5.5, CD38-PerCP-Cy5.5, CD10-PECF594, CD21-APC, IgG PeCy7, CD14-PerCP, CD123-PE, CD56-PeCy7, CD11c-APC, CD16-APC-H7 (BD Biosciences, San Diego, CA, USA), CD8-APC-EF780, CD27-APC-EF780 (eBioscience, San Diego, CA, USA), CD19-BV650, CD24-BV605 (Biolegend, San Diego, CA, USA) and IgA-PE (Miltenyi Biotech, Bergisch Gladbach, Germany). Naïve B cells were enriched by negative selection using B-cell isolation kit (Stemcell, Vancouver, BC, Canada). Naïve B-cell purity was verified by flow cytometry to 98% purity.
Cd24 bv605
Manufactured by BioLegend
Sourced in United States
CD24-BV605 is a fluorochrome-conjugated antibody that binds to the CD24 antigen. CD24 is a cell surface glycoprotein that is expressed on a variety of cell types. The BV605 fluorochrome conjugated to the antibody allows for detection and analysis of CD24-expressing cells using flow cytometry.
Automatically generated - may contain errors
Lab products found in correlation
Cd4 percp cy5, by BD (1 mentions)
Cd16 apc h7, by BD (1 mentions)
Cd27 apc ef780, by Thermo Fisher Scientific (1 mentions)
Cd56 pe cy7, by BD (1 mentions)
Cd3 apc h7, by BD (1 mentions)
Cd10 pecf594, by BD (1 mentions)
Cd21 apc, by BD (1 mentions)
Igg pe cy7, by BD (1 mentions)
Cd14 percp, by BD (1 mentions)
Cd19 bv650, by BioLegend (1 mentions)
Iga pe, by Miltenyi Biotec (1 mentions)
B cell isolation kit, by STEMCELL (1 mentions)
Cd11c apc, by BD (1 mentions)
Cd38 percp cy5, by BD (1 mentions)
Cd123 pe, by BD (1 mentions)
Cd45 apc cy7, by BD (1 mentions)
Cd34 pe cy7, by BD (1 mentions)
Cd29 apc, by BD (1 mentions)
Cd49f pe, by BD (1 mentions)
2 protocols using cd24 bv605
1
Isolation and Phenotypic Analysis of Naïve B Cells
2
Skin Sampling and Immunophenotyping Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Aseptic samples of healthy skin from surgical patients were transferred to a tube with sterile saline at 4 °C. All patients signed informed consent forms with full knowledge and donated their skin samples voluntarily. Prior approval of the local ethics committee was obtained (ChiCTR1800019082). This study was conducted in accordance with the declaration of Helsinki Principles. CD49f-PE, CD117-BV421, CD146-BV510, CD45-APC-Cy7, CD34-PE-Cy7 and CD29-APC were purchased from Becton Dickinson (San Jose, CA, USA). CD24-BV605 was from BioLegend (San Jose, CA, USA), and the TLR7 antibody (rabbit anti-human, sc-16245) was from Santa Cruz (Santa Cruz, CA, USA); it was used followed by donkey antirabbit IgG secondary antibody-Alexa Fluor 488 (Cat number: A-21206 Invitrogen, USA). Each antibody was titrated by serial dilutions. The optimized dilution of all antibodies was listed in Table 1. In total, 16 healthy donors (8 males and 8 females) were included in this study with a mean age at 26 (shown in Table 2). Abdomen includes the abdomen and groin.
Other includes the back and thighs.
[Tables 1 and2 about here]
Other includes the back and thighs.
[Tables 1 and2 about here]
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!