Apc conjugated goat anti mouse igg

Manufactured by BioLegend

APC-conjugated goat anti-mouse IgG is a secondary antibody used for the detection of mouse immunoglobulin G (IgG) in various immunoassays. The antibody is conjugated with Allophycocyanin (APC), a fluorescent dye, to facilitate the visualization and quantification of target proteins.

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Lab products found in correlation

Clone 2f4, by BioLegend (2 mentions) Vero e6, by American Type Culture Collection (2 mentions) Vero e6, by Thermo Fisher Scientific (1 mentions) Goat anti mouse igg, by BioLegend (1 mentions) Lsm780 fluorescence microscope, by Zeiss (1 mentions)

Spelling variants of this product

APC goat anti-mouse IgG (2 citations) APC)-conjugated anti-mouse IgG (2 citations) APC-conjugated goat anti-mouse IgG antibody (2 citations)

3 protocols using apc conjugated goat anti mouse igg

Generating Vero Cell Lines Expressing TMPRSS2 and ACE2

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Vero E6 (CRL-1586, American Type Culture Collection (ATCC), Vero-TMPRSS2, and Vero-hACE2-TMPRSS2 cells were cultured at 37°C in Dulbecco’s Modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 10 mM HEPES pH 7.3, 1 mM sodium pyruvate, 1× non-essential amino acids, and 100 U/ml of penicillin–streptomycin. Vero-TMPRSS2 were generated after lentivirus transduction. Briefly, human TMPRSS2 was cloned into a pLX304 lentiviral vector (gift of S. Ding, Washington University) with a C-terminal V5 tag and a blasticidin selection marker. TMPRSS2-V5-encoding vectors were packaged as lentiviruses and Vero E6 cells were transduced. Vero E6 cells stably expressing TMPRSS2 were selected under blasticidin (5 mg/mL), and surface TMPRSS2 expression was confirmed using an anti-V5 antibody (Thermo Fisher 2F11F7) or anti-TMPRSS2 mAb (Abnova, Clone 2F4) and APC-conjugated goat anti-mouse IgG (BioLegend, 405308). Vero-hACE2-TMPRSS2 were obtained as a generous gift (A. Creanga and B. Graham, NIH).

Diamond M., Chen R., Xie X., Case J., Zhang X., VanBlargan L., Liu Y., Liu J., Errico J., Winkler E., Suryadevara N., Tahan S., Turner J., Kim W., Schmitz A., Thapa M., Wang D., Boon A., Pinto D., Presti R., O’Halloran J., Kim A., Deepak P., Fremont D., Corti D., Virgin H., Crowe J., Droit L., Ellebedy A., Shi P.Y, & Gilchuk P. (2021). SARS-CoV-2 variants show resistance to neutralization by many monoclonal and serum-derived polyclonal antibodies. Research Square.

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Engineered Cell Lines for SARS-CoV-2 Research

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Vero E6 (CRL-1586, American Type Culture Collection (ATCC)), Vero-TMPRSS2⁴⁸ (gift of S. Ding, Washington University), and Vero-hACE2-TMPRSS2 (gift of A. Creanga and B. Graham, NIH) cells were cultured at 37°C in Dulbecco’s Modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 10 mM HEPES pH 7.3, 1 mM sodium pyruvate, 1× non-essential amino acids, and 100 U/ml of penicillin–streptomycin. Vero-TMPRSS2 cell cultures were supplemented with 5 μg/mL of blasticidin. TMPRSS2 expression was confirmed using an anti-V5 antibody (Thermo Fisher 2F11F7) or anti-TMPRSS2 mAb (Abnova, Clone 2F4) and APC-conjugated goat anti-mouse IgG (BioLegend, 405308). Vero-hACE2-TMPRSS2 cell cultures were supplemented with 10 μg/ml of puromycin.

Chen R.E., Zhang X., Case J.B., Winkler E.S., Liu Y., VanBlargan L.A., Liu J., Errico J.M., Xie X., Suryadevara N., Gilchuk P., Zost S.J., Tahan S., Droit L., Turner J.S., Kim W., Schmitz A.J., Thapa M., Wang D., Boon A.C., Presti R.M., O’Halloran J.A., Kim A.H., Deepak P., Pinto D., Fremont D.H., Crowe JE J.r., Corti D., Virgin H.W., Ellebedy A.H., Shi P.Y, & Diamond M.S. (2021). Resistance of SARS-CoV-2 variants to neutralization by monoclonal and serum-derived polyclonal antibodies. Nature medicine, 27(4), 717-726.

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Evaluating SMAD4 Translocation in Th17 Cells

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Naive T cells were polarized under Th17 culture condition for 24 h and then re-suspended in culture medium at 1 million/mL. $100 mL of each cell suspensions were added to a slide chamber, and spun down onto the slide using a cytocentrifuge (800 rpm/5 min). The cells were fixed on slide with 4% PFA, permeabilized with 0.01% TrionX-100, blocked with goat serum and stained with aSMAD4 antibody (Santa Cruz, Cat# sc-7966) overnight. The slides were washed and then incubated with goat anti-mouse IgG (Biolegend, Cat# 405319) or APC conjugated goat anti-mouse IgG (Biolegend, Cat# 405308) secondary antibody for 2 h at room temperature, and finally mounted with mounting medium containing DAPI. The images were obtained by LSM780 fluorescence microscope (Zeiss). The translocation ratio was measured using Image-Pro Plus 6.0 software and calculated based on the relative intensity of SMAD4 staining in the nucleus or cytoplasm.

Wang X., Ni L., Wan S., Zhao X., Ding X., Dejean A, & Dong C. (2020). Febrile Temperature Critically Controls the Differentiation and Pathogenicity of T Helper 17 Cells. Immunity, 52(2).

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