RNA isolation, cDNA synthesis, and quantitative PCR were performed as described in detail previously for cells in culture [24] . Briefly, gene expression was assessed by using the RT2 Profiler PCR Array Human Angiogenesis (PAHS-024A) from SABiosciences (Frederick, MD). Real-time PCR was performed on an ABI 7900HT Fast Real-Time PCR instrument (Applied Biosystems, Carlsbad, CA). Each tumor line was run in three biological replicates. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and β-actin (ACTB) were used as normalization genes because these housekeeping genes showed stable expression across the melanoma lines studied here. Thus, each replicate CT value was normalized to the mean CT value of GAPDH and ACTB (ΔCT = CTgene of interest – CTmean of GADPH and ACTB).
Rt2 profiler pcr array human angiogenesis pahs 024a
Manufactured by Qiagen
The RT2 Profiler PCR Array Human Angiogenesis (PAHS-024A) is a laboratory equipment product designed for the analysis of gene expression related to angiogenesis. It provides a comprehensive and standardized platform for the simultaneous quantification of multiple target genes associated with the angiogenic process.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using rt2 profiler pcr array human angiogenesis pahs 024a
1
Quantitative PCR Analysis of Angiogenesis Genes
2
Quantitative PCR Analysis of Angiogenesis Genes in Melanoma
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
RNA isolation, cDNA synthesis, and quantitative PCR were performed as described in detail previously for cells in culture [29 (link)]. Briefly, gene expression was assessed by using the RT2 Profiler PCR Array Human Angiogenesis (PAHS-024A) from SABiosciences (Frederick, MD). Real-time PCR was performed on an ABI 7900HT Fast Real-Time PCR instrument (Applied Biosystems, Carlsbad, CA). Each tumor line was run in three biological replicates. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and β-actin (ACTB) were used as normalization genes because these housekeeping genes showed stable expression across the melanoma lines studied here. Thus, each replicate CT-value was normalized to the mean CT-value of GAPDH and ACTB (ΔCT = CTgene of interest – CTmean of GADPH and ACTB).
3
Quantitative PCR Analysis of Angiogenesis Genes
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
RNA isolation, cDNA synthesis, and quantitative PCR were performed as described in detail previously [38 ]. Briefly, gene expression was assessed by using the RT2 Profiler PCR Array Human Angiogenesis (PAHS-024A) from SABiosciences (Frederick, MD). Real-time PCR was performed on an ABI 7900HT Fast Real-Time PCR instrument (Applied Biosystems, Carlsbad, CA). Each tumor line was run in three biological replicates. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and β-actin (ACTB) were used as normalization genes because these housekeeping genes showed stable expression across the melanoma lines studied here. Thus, each replicate CT-value was normalized to the mean CT-value of GAPDH and ACTB (ΔCT = CTgene of interest – CTmean of GADPH and ACTB).
4
Quantitative PCR of Angiogenesis Genes
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
RNA isolation, cDNA synthesis, and quantitative PCR were performed as described in detail previously [14 (link)]. Briefly, gene expression was assessed by using the RT2 Profiler PCR Array Human Angiogenesis (PAHS-024A) from SABiosciences (Frederick, MD). Real-time PCR was performed on an ABI 7900HT Fast Real-Time PCR instrument (Applied Biosystems, Carlsbad, CA). Each tumor line was run in three biological replicates. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and β-actin (ACTB) were used as normalization genes because these housekeeping genes showed stable expression across the melanoma lines studied here. Thus, each replicate CT value was normalized to the mean CT value of GAPDH and ACTB (ΔCT = CTgene of interest − CTmean of GADPH and ACTB). The normalized expression level of each gene was calculated from the three biological replicates as 2−meanΔCT.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!