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Sodium acetate ch3coona

Manufactured by Merck Group
Sourced in Germany, United States

Sodium acetate (CH3COONa) is a chemical compound commonly used in laboratory settings. It is a crystalline salt that dissociates in water to produce sodium and acetate ions. Sodium acetate is known for its role as a buffer solution, helping to maintain a specific pH level in various experimental procedures.

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13 protocols using sodium acetate ch3coona

1

Antioxidant Capacity Evaluation Protocol

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Distilled water was purified using a Milli–Q system (Millipore, Bedford, MA, USA). Ethanol (96%) was obtained from Vilniaus degtine (Vilnius, Lithuania). Anhydrous acetic acid (99.8%), hydrochloric acid (37%) were purchased from Sigma–Aldrich (Buchs, Switzerland). The following reagents were used: 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), 2,4,6-Tri-(2-pyridyl)-S-triazine (TPTZ), ferric chloride hexahydrate (FeCl3 × 6 H2O), sodium acetate (CH3COONa), 3-(2-pyridyl)-5,6-bis-(4-phenyl-sulfonic acid)-1,2,4-triazine (Ferrozine), obtained from Sigma-Aldrich (Buchs, Switzerland); potassium persulfate (K2S2O8), anhydrous ferrous chloride (FeCl2) from Alfa Aesar (Karlsruhe, Germany). The following standards were used: 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox), ethylene diamine tetra acetic acid (EDTA), quercetin, chlorogenic acid, cryptochlorogenic acid, ferulic acid, procyanidin A2 from Sigma–Aldrich (Buchs, Switzerland); arbutin, (+)-catechin, (−)-epicatechin from Fluka (Buchs, Switzerland); hyperoside, astragalin, rutin, isorhamnetin-3-O-glucoside, quercitrin from Extrasynthese (Genay, France); procyanidin C1, avicularin from ChromaDex (Irvine, CA, USA). All the reagents and standards were of analytical grade.
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2

Synthesis of Perovskite Oxide Nanoparticles

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Ruthenium(III)
acetylacetonate, Ru(acac)3, sodium acetate CH3COONa, CH3COOH,
Ti(OC(CH3)3)4 (TBOT), lanthanum nitrate,
La(NO3)3·xH2O, tantalum(V) chloride, TaCl5, HCl, CH3OH,
F-127 pluronic (EO106-PO70EO106,
MW 12 600 g mol–1), and C2H5OH were procured from Sigma-Aldrich.
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3

Extraction and Quantification of Organic Acids

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Sucrose, caffeic, gallic, 4‐hydroxy benzoic, tannic, and malic acids were procured from Merck (Darmstadt, Germany), and phosphoric acid (85%), sodium acetate (CH3COONa), potassium chloride, HCl (37%), and sodium hydroxide were procured from Sigma–Aldrich companies (St. Louis, USA).
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4

Linezolid-Loaded Chitosan Nanoparticles

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Linezolid (C16H20FN3O4; MW. 337.3 g/mol) of a ≥99% purity was purchased from “Beijing Mesochem Technology Co. Ltd. (Beijing, China)”. “Sodium–Tripolyphosphate (TPP), low MW chitosan (50–190 kDa, 75–85% deacetylated), and sodium acetate (CH3COONa) were purchased from Sigma Aldrich (St. Louis, MO, USA)”. “HPLC grade methanol, acetonitrile, and acetic acid glacial were procured from BDH, Ltd. (Poole, England)”. “The dialysis membrane of 12–14 kDa (MWCO) was purchased from Spectra/Por®, Spectrum Laboratories Inc., (Rancho Dominguez, CA, USA)”. “Water (purified) was obtained from Milli–Q® water purification system (Millipore, France). “All other used chemicals and solvents were of analytical and HPLC grades, respectively.
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5

Comprehensive Analysis of Bioactive Compounds

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Acetonitrile, ethyl acetate, methanol (MeOH), hexane, water HPLC grade (H2O), β-mercaptoethanol (β-ME), 2,2-diphenyl-1-picrylhydrazyl (DPPH), boric acid (H3BO3), hydrochloric acid (HCl), glutaraldehyde, osmium tetroxide, polyvinylpolypyrrolidone (PVPP), potassium hydroxide solution (KOH 0.1 M), sodium acetate (CH3COONa), sodium phosphate monobasic (NaH2PO4), sodium phosphate dibasic (Na2HPO4), Triton X-114 and uranyl acetate were acquired from Sigma-Aldrich (St. Louis, MO, USA) along with 6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox), linoleic acid, β-sitosterol, stigmasterol, campesterol, catechin, gallic acid and vanillin. Epoxy EMbed 812 resin was purchased from Electron Microscopy Sciences (Hatfield, PA, USA). Acetic acid (CH3COOH), ethanol (EtOH), sodium carbonate (Na2CO3) and KOH were purchased from DEQ (Monterrey, Nuevo León, Mexico).
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6

Artificial Cotton Fiber Functionalization Protocol

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ACFs (STF-1600, 100% viscose base, commonly known as artificial cotton) were provided by Sutong Carbon Fiber Co., Ltd. (Jiangsu, China). Dopamine hydrochloride (product #: H8502, CAS: 62−31−7), sodium acetate (CH3COONa, product #: 32319, CAS:127-09-3), Tween® 20 (product #: P9416, CAS:9005-64-5), copper sulfate pentahydrate (CuSO4∙5H2O, product #: 31293, CAS:7758-99-8), and phosphate-buffered saline (PBS, product #: P4417, PCode:1002852269) were purchased from Sigma Aldrich (St. Louis, MO, USA). BBLTM eosin methylene blue agar, modified (Holt–Harris and Teague) was bought from Becton, Dickinson and Company (Franklin Lakes, NJ, USA). Luria Bertani (LB) broth (Miller’s) solution (10 g of tryptone, 5 g of yeast extract, and 10 g of NaCl dissolved in 1 L of deionized water) was provided by Genomicbase (Seoul, South Korea). Agar powder was supplied by Duksan Chemicals Co. Ltd. (Ansan, South Korea).
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7

Graphene-based Electrochemical Biosensor Fabrication

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Graphite is purchased from
Fisher Scientific (size <45.0 μm). Sodium nitrate (NaNO3, 99%), potassium permanganate (KMnO4, 99%), sulfuric
acid (H2SO4, 98%, d = 1.83
g/cm3), o-Phosphoric acid (H3PO4,
85%, d = 1.71 g/cm3), hydrogen peroxide (H2O2, 35 wt %), human Hb (catalog #H7379), iron (II) chloride
(FeCl2, 99%), and iron(III) chloride (FeCl3,
99%) are used in this study. Potassium ferrocyanide, K4[Fe(CN)6], potassium ferricyanide, K3[Fe(CN)6], potassium chloride (KCl, 99.9%), sodium acetate (CH3COONa, Reagent Plus, 99.0%), and glacial acetic acid (CH3COOH, 100%, d = 1.04 g/cm3) are
purchased from Sigma-Aldrich. Acetate buffer (0.1 M, pH = 4.5) is
freshly prepared and used as the electrolyte to run all experiments.
The chemicals used are of analytical grade. All solutions were prepared
using Milli-Q ultra-pure water (conductance = 17.5 MΩ).
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8

Multifunctional Nanodiamonds for Cancer Therapy

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All chemicals and reagents were used without further purification unless otherwise noted. The nanodiamonds were purchased from Sino Crytal Micro Diamond Co. Ltd. (Zhengzhou, China). Ferric chloride hexahydrate (FeCl3·6H2O), morpholine ethyl sulfonic acid (MES), ethylene glycol (CH2OHCH2OH), polyethylene glycol, (3-aminopropyl)-trimethoxy silane (APTES), and sodium acetate (CH3COONa) were purchased from Sigma-Aldrich (Shanghai, China) and used as received. Doxorubicin hydrochloride (DOX·HCl) was supplied by Shanghai Jinhe Bio-Technology Co. Ltd (Shanghai, China). Dulbecco’s minimum essential medium (DMEM) cell culture medium, penicillin/streptomycin (PS), and fetal bovine serum (FBS) were bought from Gibco Invitrogen (New York, USA). Human lung adenocarcinoma A549 cells were purchased from the Chinese Academy of Sciences Cell Bank. Cell Counting Kit-8 (CCK-8 assay), 4′,6-diamidino-2-phenylindole (DAPI), Calcein-AM and propidium iodide (PI) were purchased from Summus (Zhengzhou, China). Deionized (DI) water was freshly obtained using the Milli-Q water purification system (Millipore, Bedford, MA, USA).
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9

Synthesis and Characterization of Metal-Organic Frameworks

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2-Aminoterephthalic acid H2N-C6H3-1,4-(COOH)2 (NH2-TPA) (99 wt%), iron(iii) chloride hexahydrate FeCl3·6H2O (99 wt%), methanol CH3OH (MeOH) (anhydrous, 99.8 v/v%), sodium acetate CH3COONa (99 wt%), acetic acid CH3COOH (99.5 wt%), cadmium nitrate tetrahydrate Cd(NO3)2·4H2O (99 wt%), lead nitrate Pb(NO3)2 (99 wt%), and copper sulfate pentahydrate CuSO4·5H2O (99 wt%.) were purchased from Sigma-Aldrich. Cobalt sulphate heptahydrate (CoSO4·7H2O 99.5 wt%), nickel sulphate hexahydrate (NiSO4·6H2O 98.5 wt%), potassium dichromate (K2Cr2O7 99.8 wt%), and zinc chloride (ZnCl2 98 wt%.) were supplied by Xilong Chemical Co., China. The appropriate amounts of sodium acetate and acetic acid were used to prepare acetate buffer solution 0.1 M (ABS) with pH 6.0. Dimethylformamide C3H7NO (DMF) (99.94 v/v%) was purchased from Fisher Chemical. Polyethylene glycol 2000 (H(OCH2CH2)nOH, PEG 2000) was supplied by Alfa Aesar.
A Siemens D5005 diffractometer (Cu Kα radiation, λ = 1.54056 Å), a NICOLET iS50FT-IR spectrometer, a Hitachi S4800 scanning electron microscope (SEM), and a TECNAI F20-G2 high-resolution transmission electron microscope (TEM) were used to perform X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FT-IR), SEM, and TEM measurements, respectively. Electrochemical measurements were conducted using a PGSTAT302N AutoLab electrochemical workstation (Metrohm, Netherlands).
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10

Biophysical Characterization of Molecular Interactions

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Bovine serum albumine (fatty acid-free; LOT number: SLCB1005), hen egg-white lysozyme (LOT number: K49054981), sodium polystyrene sulfonate (average molecular weight 70,000 g/mol), polyethylene glycols with an average molecular weight of 400 g/mol (PEG-400) and 3000 g/mol (PEG-3000), sucrose, sucralose, and NaI (>99%) were purchased from Sigma Aldrich, Burlington, MA, USA. Alkaline metal salts of >99% purity (NaCl, NaBr), concentrated acetic acid (CH3COOH), sodium acetate (CH3COONa), and 1 mol/L NaOH solution were purchased from Merck KGaA. Polyethylene glycol with an average molecular weight of 20,000 g/mol (PEG-20000) was obtained from Fluka.
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