Apelin 12 enzyme immunoassay eia kit

Following 5-hour fasting, blood for biochemical analysis was collected from heart via cardiopuncture under anesthesia and used for measuring insulin and glucose levels. Fetal blood was further collected in accordance with a previous report (16 (link)). Serum apelin and insulin concentrations were measured using an apelin-12 enzyme immunoassay (EIA) kit (Phoenix Pharmaceuticals, Belmont, CA) and a mouse high range insulin enzyme-linked immunosorbent assay kit (ALPCO, Salem, NH), and blood glucose was measured using a glucose meter (Contour Blood Glucose Meter). The homeostatic model assessment of insulin resistance (HOMA-IR) and pancreatic β-cell function (HOMA-%B) were calculated in accordance with the following formula: HOMA-IR [fasting insulin in μU/ml × (fasting blood glucose in mg/dl × 0.055)/22.5]; HOMA-%B [(360 × fasting insulin in μU/ml)/(fasting blood glucose − 63)] (16 (link)).